Spermatic and testicular damages in rats exposed to ethanol: Influence of lipid peroxidation but not testosterone

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dc.contributor.authorSiervo, Glaucia E. M. L.
dc.contributor.authorVieira, Henrique R.
dc.contributor.authorOgo, Fernanda M.
dc.contributor.authorFernandez, Carla D. B. [UNESP]
dc.contributor.authorGoncalves, Gessica D.
dc.contributor.authorMesquita, Suzana F. P.
dc.contributor.authorAnselmo-Franci, Janete Ap.
dc.contributor.authorCecchini, Rubens
dc.contributor.authorGuarnier, Flavia A.
dc.contributor.authorFernandes, Glaura S. A.
dc.contributor.institutionUniversidade Estadual de Londrina (UEL)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2015-10-21T13:11:41Z
dc.date.available2015-10-21T13:11:41Z
dc.date.issued2015-04-01
dc.description.abstractChronic consumption of ethanol causes morphological and physiological changes in the reproductive system of mammals. Vitamin C has an antioxidant role in organisms by neutralizing the ROS (reactive oxygen species) produced by oxidizing agents and this vitamin has an important function in the male reproductive system. The aim of this study was to evaluate whether vitamin C could prevent or attenuate the alterations in the male reproductive system caused by ethanol consumption. To test this hypothesis, male rats were divided into three experimental groups and treated by gavage for 63 days. The ethanol (E) and ethanol +vitamin C (EC) groups received 2 g/kg of ethanol (25% v/v) daily. In addition to ethanol, the EC group received vitamin C at a dose of 100 mg/day, diluted in water. The control group (C) received only the vehicle. On the 64th experimental day, the animals were anesthetized and euthanized, and blood was collected for plasmatic hormonal analysis. The testis, epididymis, vas deferens, and seminal vesicles were removed and weighed. Sperm from the vas deferens was submitted to morphological and motility analysis. The testis and epididymis were used for oxidative stress and histopathological analysis, sperm count, morphometric analysis of the testis, and stereological analysis of the epididymis. The results showed that vitamin C has a protective effect in the testes of adult male rats, entirely normalizing the parameters of sperm count, spermatogenesis kinetics, lipid peroxidation levels, and sperm motility, as well as partially normalizing the histopathological damage in the testis, epididymis, and sperm morphology. Thus, we concluded that lipid peroxidation is a major mechanism by which ethanol affects the testes and sperm, whereas no plasmatic testosterone alterations were found.en
dc.description.affiliationUniversidade Estadual Paulista (UNESP) - Department of Morphology, Institute of Biosciences, Botucatu, São Paulo, Brazil
dc.description.affiliationState University of Londrina (UEL) - Department of General Biology, Biological Sciences Center, Londrina, Paraná, Brazil
dc.description.affiliationUniversity of São Paulo (USP) - Department of Morphology, Stomatology and Physiology, Dental School of Ribeirão Preto, Ribeirão Preto, São Paulo, Brazil
dc.description.affiliationState University of Londrina, Department of General Pathology, Biological Sciences Center, Londrina, Paraná, Brazil
dc.description.affiliationUnespUniversidade Estadual Paulista (UNESP) - Department of Morphology, Institute of Biosciences, Botucatu, São Paulo, Brazil
dc.format.extent1-8
dc.identifierhttp://www.sciencedirect.com/science/article/pii/S0300483X15000244
dc.identifier.citationToxicology. Clare: Elsevier Ireland Ltd, v. 330, p. 1-8, 2015.
dc.identifier.doi10.1016/j.tox.2015.01.016
dc.identifier.issn0300-483X
dc.identifier.urihttp://hdl.handle.net/11449/128630
dc.identifier.wosWOS:000351803400001
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofToxicology
dc.relation.ispartofjcr3.265
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectTestisen
dc.subjectEpididymisen
dc.subjectSpermen
dc.subjectEthanolen
dc.subjectVitamin Cen
dc.subjectLipid peroxidationen
dc.titleSpermatic and testicular damages in rats exposed to ethanol: Influence of lipid peroxidation but not testosteroneen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.
unesp.author.orcid0000-0002-6480-1187[7]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt

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