Molecular and biochemical characterization of the Neurospora crassa glycogen synthase encoded by the gsn cDNA

Página do item simplificado
dc.contributor.authorde Paula, R.
dc.contributor.authorde Pinho, C. A.
dc.contributor.authorTerenzi, H. F.
dc.contributor.authorBertolini, Maria Celia [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2014-05-20T15:20:35Z
dc.date.available2014-05-20T15:20:35Z
dc.date.issued2002-04-01
dc.description.abstractGlycogen synthases catalyze the transfer of a glucosyl moiety from a nucleotide phosphosugar to a nascent glycogen chain via an alpha1-->4 linkage. Although many genes coding for glycogen synthases have been described, the enzymes from rabbit and yeast are the best characterized. The fungus Neurospora crassa accumulates glycogen during exponential growth, and mobilizes it at the onset of stationary phase, or when placed at high temperature or starved for carbon. Through a PCR methodology, the gsn cDNA coding for the N. crassa glycogen synthase was isolated, and the amino acid sequence of the protein was deduced. The product of the cDNA seems to be the only glycogen synthase present in N. crassa. Characterization of the gsn cDNA revealed that it codes for a 706-amino acids protein, which is very similar to mammalian and yeast glycogen synthases. Gene expression increased during exponential growth, reaching its maximal level at the end of the exponential growth phase, which is consistent with the pattern of glycogen synthase activity and glycogen level. Expression of the gsn is highly regulated at the transcriptional level. Under culture conditions that induce heat shock, conidiation, and carbon starvation, expression of the gsn gene was decreased, and glycogen synthase activity and glycogen content behaved similarly.en
dc.description.affiliationUNESP, Inst Quim, Dept Bioquim & Tecnol Quim, BR-14800900 Araraquara, SP, Brazil
dc.description.affiliationUSP, Dept Biol, Fac Filosofia Ciências & Letras, BR-14040901 Ribeirao Preto, SP, Brazil
dc.description.affiliationUnespUNESP, Inst Quim, Dept Bioquim & Tecnol Quim, BR-14800900 Araraquara, SP, Brazil
dc.format.extent241-253
dc.identifierhttp://dx.doi.org/10.1007/s00438-002-0659-4
dc.identifier.citationMolecular Genetics and Genomics. New York: Springer-verlag, v. 267, n. 2, p. 241-253, 2002.
dc.identifier.doi10.1007/s00438-002-0659-4
dc.identifier.issn1617-4615
dc.identifier.lattes8817669953838863
dc.identifier.urihttp://hdl.handle.net/11449/31856
dc.identifier.wosWOS:000175885800013
dc.language.isoeng
dc.publisherSpringer
dc.relation.ispartofMolecular Genetics and Genomics
dc.relation.ispartofjcr2.734
dc.relation.ispartofsjr1,168
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectNeurospora crassapt
dc.subjectcDNApt
dc.subjectglycogen synthase activitypt
dc.subjectglycogenpt
dc.titleMolecular and biochemical characterization of the Neurospora crassa glycogen synthase encoded by the gsn cDNAen
dc.typeArtigo
dcterms.licensehttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dcterms.rightsHolderSpringer
unesp.author.lattes8817669953838863
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Química, Araraquarapt

Arquivos

Licença do Pacote
Agora exibindo 1 - 1 de 1
Nenhuma Miniatura disponível
Nome:
license.txt
Tamanho:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descrição:

Coleções

Artigos - Bioquímica e Tecnologia - IQ