Structure of a glycoglucuronomannan from the low-viscosity gum of Vochysia lehmannii

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dc.contributor.authorWagner, R.
dc.contributor.authorWoranovicz-Barreira, S. M.
dc.contributor.authorIacomini, M.
dc.contributor.authorDelgobo, C. L.
dc.contributor.authorPimentel, N. M.
dc.contributor.authorGorin, PAJ
dc.contributor.institutionUniversidade Federal do Paraná (UFPR)
dc.contributor.institutionUniversidade Federal de Santa Catarina (UFSC)
dc.contributor.institutionSEPROF
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T15:25:49Z
dc.date.available2014-05-20T15:25:49Z
dc.date.issued2004-09-13
dc.description.abstractThe polysaccharide (VSP) from the gum exudate of quaruba (Vochysia lehmannii) had two components of almost identical M. centred at 24,800, as shown by HSPEC-MALLS. The presence of aggregates was shown since carboxy-reduction gave VSP-RED, which contained low molecular weight components with M-w 19,000 > 5800 and polydispersity ratios dn/dc 0.160 and 0.149, respectively. VSP formed low viscosity aqueous solutions and acid hydrolysis gave Man (30%), Ara (16%), Gal (10%), and Glc (14%). The latter arose partly from GlcA (30%). Methylation analysis revealed mainly neutral units of 2-O- (60%) and 2,3-di-O-substituted Manp (5%), and those of nomeducing ends (8%), 2-O- (3%), and 4-O-substituted Arap and/or 5-O-substituted Araf units (6%). VSP-RED contained Glc (45%), Man (35%), and Ara (13%) and methylation analysis indicated mainly 4-O-substituted Glcp (31%) and 2-O- (51%) and 2,3-di-O-substituted Manp units (5%). A predominant alternating structure for VSP was shown by its C-13 NMR spectrum, which contained 10 main signals and a small one of C-6 of GlcpA. This was confirmed by formation, on partial hydrolysis of VSP, of a tetrasaccharide, which was characterised by NMR spectroscopy and ESI-MS as beta-GlcpA-(1 --> 2)-alpha-Manp-(1 --> 4)-beta-GlcpA-(1 --> 2)-Man, which arose from the main chain, thus confirming VSP to be a glycoglucuronomannan. (C) 2004 Elsevier Ltd. All rights reserved.en
dc.description.affiliationUniversidade Federal do Paraná (UFPF), Dept Bioquim, BR-81231990 Curitiba, Parana, Brazil
dc.description.affiliationUniversidade Federal do Paraná (UFPF), Dept Farmacia, BR-80210170 Curitiba, Parana, Brazil
dc.description.affiliationUniv Fed Santa Catarina, Dept Analises Clin, BR-88040970 Florianopolis, SC, Brazil
dc.description.affiliationSEPROF, BR-69900600 Rio Branco, AC, Brazil
dc.description.affiliationUniv Estadual Paulista, BR-18603970 Botucatu, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, BR-18603970 Botucatu, SP, Brazil
dc.format.extent269-275
dc.identifierhttp://dx.doi.org/10.1016/j.carbpol.2004.05.004
dc.identifier.citationCarbohydrate Polymers. Oxford: Elsevier B.V., v. 57, n. 3, p. 269-275, 2004.
dc.identifier.doi10.1016/j.carbpol.2004.05.004
dc.identifier.issn0144-8617
dc.identifier.urihttp://hdl.handle.net/11449/36153
dc.identifier.wosWOS:000223822200005
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofCarbohydrate Polymers
dc.relation.ispartofjcr5.158
dc.relation.ispartofsjr1,428
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectVochysia lehmanniipt
dc.subjectgum polysaccharidept
dc.subjectaggregatespt
dc.subjectlow viscositypt
dc.subjectglycoglucuronomannanpt
dc.subjectAraf side chainspt
dc.titleStructure of a glycoglucuronomannan from the low-viscosity gum of Vochysia lehmanniien
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.

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