Relevance of the Myeloid Differentiation Factor 88 (MyD88) on RANKL, OPG, and Nod Expressions Induced by TLR and IL-1R Signaling in Bone Marrow Stromal Cells

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dc.contributor.authorManzolli Leite, Fabio Renato [UNESP]
dc.contributor.authorAquino, Sabrina Garcia de [UNESP]
dc.contributor.authorGuimaraes, Morgana Rodrigues [UNESP]
dc.contributor.authorCirelli, Joni Augusto [UNESP]
dc.contributor.authorZamboni, Dario S.
dc.contributor.authorSilva, Joao S.
dc.contributor.authorRossa Junior, Carlos [UNESP]
dc.contributor.institutionUniversidade Federal de Pernambuco (UFPE)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2015-10-21T13:10:39Z
dc.date.available2015-10-21T13:10:39Z
dc.date.issued2015-02-01
dc.description.abstractThe myeloid differentiation factor 88 (MyD88) plays a pivotal role in Toll-like receptor (TLR)- and interleukin-1 receptor (IL-1R)-induced osteoclastogenesis. We examined the role of MyD88 on p38 mitogen-activated protein kinase (MAPK) and nuclear factor kappa-light-chain-enhancer of activated B cell (NF-kappa B) activation and nucleotide-binding oligomerization domain (Nod) induction by lipopolysaccharide (LPS) and IL-1 beta, and their effect on receptor activator of NF-kappa B ligand (RANKL) and osteoprotegerin (OPG) production in bone marrow stromal cell (BMSC). RANKL, Nod1, Nod2, NF-kappa B, and p38 protein levels were determined by Western blot. Nod2 was stimulated with muramyl dipeptide (MDP) prior to TLR4 stimulation with LPS. MyD88 deficiency markedly inhibited RANKL expression after LPS stimulation and increased OPG messenger RNA (mRNA) production. Also, MyD88 was necessary for NF-kappa B and p38 MAPK activation. MDP alone did not induce RANKL and OPG expressions; however, when combined with LPS, their expressions were significantly increased (p < 0.05). Our results support that MyD88 signaling has a pivotal role in osteoclastogenesis thought NF-kappa B and p38 activation. Nod2 and especially Nod1 levels were influenced by MyD88.en
dc.description.affiliationFed Univ Pelotas UFPel, Sch Dent, Pelotas, RS, Brazil
dc.description.affiliationState Univ Sao Paulo UNESP, Dept Diag &Surg, Sch Dent Araraquara, BR-14801903 Sao Paulo, Brazil
dc.description.affiliationUniv Sao Paulo, Dept Cell Biol &Microbial Pathogenesis, Sch Med, BR-09500900 Sao Paulo, Brazil
dc.description.affiliationUniv Sao Paulo, Dept Biochem &Immunol, Sch Med, BR-09500900 Sao Paulo, Brazil
dc.description.affiliationUnespState Univ Sao Paulo UNESP, Dept Diag &Surg, Sch Dent Araraquara, BR-14801903 Sao Paulo, Brazil
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdCAPES: 3675/07-6
dc.description.sponsorshipIdFAPESP: 05-04247-4
dc.description.sponsorshipIdFAPESP: 05-04351-6
dc.format.extent1-8
dc.identifier.citationInflammation, v. 38, n. 1, p. 1-8, 2015.
dc.identifier.doi10.1007/s10753-014-0001-4
dc.identifier.issn0360-3997
dc.identifier.lattes2628593693450121
dc.identifier.urihttp://hdl.handle.net/11449/128525
dc.identifier.wosWOS:000349015900001
dc.language.isoeng
dc.publisherSpringer
dc.relation.ispartofInflammation
dc.relation.ispartofjcr2.884
dc.relation.ispartofsjr1,023
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectlipopolysaccharideen
dc.subjectsignaling pathwayen
dc.subjectmitogen-activated protein kinasesen
dc.subjectnuclear factor kappa-light-chain-enhancer of activated B cellsen
dc.subjectreceptor activator for nuclear factor kappa B liganden
dc.subjectosteoprotegerinen
dc.titleRelevance of the Myeloid Differentiation Factor 88 (MyD88) on RANKL, OPG, and Nod Expressions Induced by TLR and IL-1R Signaling in Bone Marrow Stromal Cellsen
dc.typeArtigo
dcterms.licensehttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dcterms.rightsHolderSpringer
unesp.author.lattes2628593693450121
unesp.author.lattes7634063102292261[7]
unesp.author.orcid0000-0003-1705-5481[7]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Odontologia, Araraquarapt

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