Effect of soybean oil and Tween 80 on the production of botryosphaeran by Botryosphaeria rhodina MAMB-05

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dc.contributor.authorSilva, Cassiano C.
dc.contributor.authorDekker, Robert F. H.
dc.contributor.authorSilva, Rui Sergio S. F.
dc.contributor.authorda Silva, Maria de Lourdes Corradi
dc.contributor.authorBarbosa, Aneli M.
dc.contributor.institutionUniversidade Estadual de Londrina (UEL)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T13:22:54Z
dc.date.available2014-05-20T13:22:54Z
dc.date.issued2007-08-01
dc.description.abstractThe addition of soybean oil and Tween 80 was evaluated with the objective of increasing the production of botryosphaeran, an exopolysaccharide (EPS) of the (1 -> 3 ;1 -> 6)-beta-D-glucan type produced by the fungus Botowsphaeria rhodina MAMB-05. Factorial design and analysis by response surface methodology was developed to select the main factors that would affect and enhance EPS production. The optimized culture conditions were: 40g l(-1) glucose with 10ml l(-1) soybean oil, and 4.5 ml l(-1) Tween 80, during 72h cultivation at 28 degrees C (180 rpm) and initial pH 5.7. The predicted result for botryosphaeran production was 8.22 +/- 1.36 g l(-1), and compared with the experimental value of 7.74 +/- 0.13 g l(-1) . Partial characterization of the botryosphaeran produced under the optimized conditions showed one type of polysaccharide with P-glycosidic linkages containing glucose as monosaccharide. (c) 2007 Elsevier Ltd. All rights reserved.en
dc.description.affiliationUniv Estadual Londrina, Dept Bioquim & Biotecnol CCE, BR-86051990 Londrina, PR, Brazil
dc.description.affiliationUniv Estadual Londrina, Dept Ciência & Tecnol Alimentos, CCA, BR-86051990 Londrina, PR, Brazil
dc.description.affiliationUniv Estadual Paulista, Fac Ciências & Tecnol, Dept Fis Quim & Biol, BR-19060900 Presidente Prudente, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Fac Ciências & Tecnol, Dept Fis Quim & Biol, BR-19060900 Presidente Prudente, SP, Brazil
dc.format.extent1254-1258
dc.identifierhttp://dx.doi.org/10.1016/j.procbio.2007.05.009
dc.identifier.citationProcess Biochemistry. Oxford: Elsevier B.V., v. 42, n. 8, p. 1254-1258, 2007.
dc.identifier.doi10.1016/j.procbio.2007.05.009
dc.identifier.issn1359-5113
dc.identifier.urihttp://hdl.handle.net/11449/6797
dc.identifier.wosWOS:000248975400011
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofProcess Biochemistry
dc.relation.ispartofjcr2.616
dc.relation.ispartofsjr0,761
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectbotryosphaeranpt
dc.subjectBotryosphaeria rhodina MAMB-05pt
dc.subjectexopolysaccharidept
dc.subjectsoybean oilpt
dc.subjecttween 80pt
dc.subjectresponse surface methodologypt
dc.titleEffect of soybean oil and Tween 80 on the production of botryosphaeran by Botryosphaeria rhodina MAMB-05en
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.
unesp.author.orcid0000-0002-2339-8985[5]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências e Tecnologia, Presidente Prudentept

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Artigos - Física, Química e Biologia - FCT