Catecholamine effects on human melanoma cells evoked by α1-adrenoceptors

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dc.contributor.authorScarparo, Ana Cristina
dc.contributor.authorSumida, Doris Hissako [UNESP]
dc.contributor.authorPatrão, Marília T. C. C.
dc.contributor.authorAvellar, Maria Christina W.
dc.contributor.authorVisconti, Maria Aparecida
dc.contributor.authorCastrucci, Ana Maria de Lauro
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2014-05-27T11:21:07Z
dc.date.available2014-05-27T11:21:07Z
dc.date.issued2004-08-01
dc.description.abstractThe biological effects of catecholamines in mammalian pigment cells are poorly understood. Our previous results showed the presence of α1-adrenoceptors in SK-Mel 23 human melanoma cells. The aims of this work were to (1) characterize catecholamine effects on proliferation, tyrosinase activity and expression, (2) identify the α1- adrenoceptor subtypes, and (3) verify whether chronic norepinephrine (NE) treatment modified the types and/or pharmacological characteristics of adrenoceptors present in SK-Mel 23 human melanoma cells. Cells treated with the aradrenergic agonist, phenylephrine (PHE, 10-5 or 10-4 M), for 24-72 h, exhibited decreased cell proliferation and enhanced tyrosinase activity, but unaltered tyrosinase expression as compared with the control. The proliferation and tyrosinase activity responses were inhibited by the α1-adrenergic antagonist prazosin, suggesting they were evoked by α1-adrenoceptors. The presence of actinomycin D, a transcription inhibitor, did not diminish PHE-induced effects. RT-PCR assays, followed by cloning and sequencing, demonstrated the presence of α1A- and α1B-adrenoceptor subtypes. NE-treated cells (24 or 72 h) were used in competition assays, and showed no significant change in the competition curves of α1-adrenoceptors as compared with control curves. Other adrenoceptor subtypes were not identified in these cells, and NE pretreatment did not induce their expression. In conclusion, the activation of SK-Mel 23 human melanoma α1- radrenoceptors elicit biological effects, such as proliferation decrease and tyrosinase activity increase. Desensitization or expression of other adrenoceptor subtypes after chronic NE treatment were not observed.en
dc.description.affiliationDepartamento de Fisiologia Institute de Biociências Universidade de São Paulo, R. do Matão, trav. 14, 321, CEP 05508-900 São Paulo
dc.description.affiliationDepartamento de Ciencias Basicas Faculdade de Odontologia Universidade Estadual de Sao Paulo, Araçatuba, São Paulo
dc.description.affiliationSect. of Experimental Endocrinology Department of Pharmacology Univ. Fed. S. P.-Esc. Paulista Med., São Paulo
dc.description.affiliationUnespDepartamento de Ciencias Basicas Faculdade de Odontologia Universidade Estadual de Sao Paulo, Araçatuba, São Paulo
dc.format.extent112-119
dc.identifierhttp://dx.doi.org/10.1007/s00403-004-0488-x
dc.identifier.citationArchives of Dermatological Research, v. 296, n. 3, p. 112-119, 2004.
dc.identifier.doi10.1007/s00403-004-0488-x
dc.identifier.issn0340-3696
dc.identifier.lattes8275401688702343
dc.identifier.scopus2-s2.0-4344634280
dc.identifier.urihttp://hdl.handle.net/11449/67806
dc.language.isoeng
dc.relation.ispartofArchives of Dermatological Research
dc.relation.ispartofjcr2.148
dc.relation.ispartofsjr1,006
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectα1-Adrenoceptors
dc.subjectCatecholamines
dc.subjectCell proliferation
dc.subjectSK-Mel 23 melanoma cells
dc.subjectTyrosinase activity
dc.subjectalpha 1 adrenergic receptor
dc.subjectalpha 1 adrenergic receptor blocking agent
dc.subjectalpha 1 adrenergic receptor stimulating agent
dc.subjectascorbic acid
dc.subjectbenoxathian
dc.subjectcatecholamine
dc.subjectdactinomycin
dc.subjectmonophenol monooxygenase
dc.subjectnoradrenalin
dc.subjectphenylephrine
dc.subjectprazosin
dc.subjectadrenergic system
dc.subjectcatecholamine metabolism
dc.subjectcell proliferation
dc.subjectcell strain SK Mel 23
dc.subjectcontrolled study
dc.subjectenzyme activity
dc.subjecthuman
dc.subjecthuman cell
dc.subjectmelanoma cell
dc.subjectpigment cell
dc.subjectpriority journal
dc.subjectreverse transcription polymerase chain reaction
dc.subjectWestern blotting
dc.subjectAdrenergic alpha-Agonists
dc.subjectBinding, Competitive
dc.subjectCell Division
dc.subjectCell Line, Tumor
dc.subjectGene Expression
dc.subjectHumans
dc.subjectMelanoma
dc.subjectMonophenol Monooxygenase
dc.subjectNorepinephrine
dc.subjectPhenylephrine
dc.subjectReceptors, Adrenergic, alpha-1
dc.subjectSkin Neoplasms
dc.titleCatecholamine effects on human melanoma cells evoked by α1-adrenoceptorsen
dc.typeArtigo
dcterms.licensehttp://www.springer.com/open+access/authors+rights
unesp.author.lattes8275401688702343
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Odontologia, Araçatubapt

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