Synthesis and evaluation of diethylethylamine-chitosan for gene delivery: Composition effects on the in vitro transfection efficiency

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dc.contributor.authorDe Paula Pansani Oliveira, Franciele [UNESP]
dc.contributor.authorPicola, Isadora Pfeifer Dalla [UNESP]
dc.contributor.authorShi, Qin
dc.contributor.authorBarbosa, Hellen Franciane Gonçalves [UNESP]
dc.contributor.authorDe Oliveira Tiera, Vera Aparecida [UNESP]
dc.contributor.authorFernandes, Júlio Cesar
dc.contributor.authorTiera, Marcio José [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversité de Montréal
dc.date.accessioned2014-05-27T11:28:22Z
dc.date.available2014-05-27T11:28:22Z
dc.date.issued2013-02-08
dc.description.abstractChitosan has been indicated as a safe and promising polycation vector for gene delivery. However its low transfection efficiency has been a challenging obstacle for its application. To address this limitation, we synthesized chitosan derivatives which had increasing amounts of diethylethylamine groups (DEAE) attached to the chitosan main chain. The plasmid DNA VR1412 (pDNA), encoding the ß-galactosidase (ß-gal) reporter gene was used to prepare nanoparticles with the chitosan derivatives, and the transfection studies were performed with HeLa cells. By means of dynamic light scattering and zeta potential measurements, it was shown that diethylethylamine-chitosan derivatives (DEAEx-CH) were able to condense DNA into small particles having a surface charge depending on the polymer/DNA ratio (N/P ratio). Nanoparticles prepared with derivatives containing 15 and 25% of DEAE groups (DEAE15-CH and DEAE25-CH) exhibited transfection efficiencies ten times higher than that observed with deacetylated chitosan (CH). For derivatives with higher degrees of substitution (DS), transfection efficiency decreased. The most effective carriers showed low cytotoxicity and good transfection activities at low charge ratios (N/P). Vectors with low DS were easily degraded in the presence of lysozyme at physiological conditions in vitro and the nontoxicity displayed by these vectors opens up new opportunities in the design of DEAE-chitosan-based nanoparticles for gene delivery. © 2013 IOP Publishing Ltd.en
dc.description.affiliationDepartment of Chemistry and Environmental Sciences IBILCE São Paulo State University UNESP, São José do Rio Preto, São Paulo
dc.description.affiliationOrthopedic Research Laboratory Hôpital du Sacré-Cœur de Montréal Université de Montréal
dc.description.affiliationDepartment of Physics IBILCE São Paulo State University UNESP, São José do Rio Preto, São Paulo
dc.description.affiliationUnespDepartment of Chemistry and Environmental Sciences IBILCE São Paulo State University UNESP, São José do Rio Preto, São Paulo
dc.description.affiliationUnespDepartment of Physics IBILCE São Paulo State University UNESP, São José do Rio Preto, São Paulo
dc.identifierhttp://dx.doi.org/10.1088/0957-4484/24/5/055101
dc.identifier.citationNanotechnology, v. 24, n. 5, 2013.
dc.identifier.doi10.1088/0957-4484/24/5/055101
dc.identifier.issn0957-4484
dc.identifier.issn1361-6528
dc.identifier.lattes8796747160088337
dc.identifier.scopus2-s2.0-84872382611
dc.identifier.urihttp://hdl.handle.net/11449/74572
dc.identifier.wosWOS:000313568700002
dc.language.isoeng
dc.relation.ispartofNanotechnology
dc.relation.ispartofjcr3.404
dc.relation.ispartofsjr1,079
dc.relation.ispartofsjr1,079
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectCharge ratio
dc.subjectChitosan derivatives
dc.subjectDeacetylated chitosans
dc.subjectDegrees of substitution
dc.subjectGalactosidases
dc.subjectGene Delivery
dc.subjectHeLa cell
dc.subjectIn-vitro
dc.subjectMain chains
dc.subjectNon-toxicity
dc.subjectPhysiological condition
dc.subjectPlasmid DNA
dc.subjectPolycations
dc.subjectReporter gene
dc.subjectSmall particles
dc.subjectTransfection activity
dc.subjectTransfection efficiency
dc.subjectZeta potential measurements
dc.subjectEfficiency
dc.subjectGene encoding
dc.subjectGene transfer
dc.subjectNanoparticles
dc.subjectZeta potential
dc.subjectChitosan
dc.titleSynthesis and evaluation of diethylethylamine-chitosan for gene delivery: Composition effects on the in vitro transfection efficiencyen
dc.typeArtigo
dcterms.licensehttp://iopscience.iop.org/page/copyright
unesp.author.lattes8796747160088337
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências Letras e Ciências Exatas, São José do Rio Pretopt

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Artigos - Química e Ciências Ambientais - IBILCE
Artigos - Física - IBILCE