Intra-examiner reproducibility of the agar plate method for estmation of phospholipase activity in candida albicans

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dc.contributor.authorDovigo, Lívia Nordi [UNESP]
dc.contributor.authorSanitá, Paula Volpato [UNESP]
dc.contributor.authorCampos, Juliana Alvares Duarte Bonini [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2015-08-06T16:13:56Z
dc.date.available2015-08-06T16:13:56Z
dc.date.issued2013
dc.description.abstractAlthough measurement errors can impair statistical analysis, reliability analysis has been neglected in applied microbiology. This study assessed the intra-rater reproducibility of the Agar-based method for estimation of phospholipase activity (Pz). Pz readings were performed twice by two examiners (E1, E2), either directly on plates or in photos, and both black and white backgrounds were used. Pz values were taken from one or triplicate colonies from each sample (n=30). Intra-examiner reproducibility was estimated using Intraclass Correlation Coefficient (ICC). For both examiners, reading triplicate (ICCE1=0.91, ICCE2=0.86) was better than one colony (ICCE1=0.86, ICCE2=0.80). E1 had an excellent concordance when measurements were performed on photos using a white background (ICC=0.95) and a good concordance in the other conditionsen
dc.description.affiliationUniversidade Estadual Paulista Júlio de Mesquita Filho, Faculdade de Odontologia de Araraquara, Araraquara, Rua Humaitá, 1680, Centro, CEP 14801-903, SP, Brasil
dc.description.affiliationUnespUniversidade Estadual Paulista Júlio de Mesquita Filho, Faculdade de Odontologia de Araraquara, Araraquara, Rua Humaitá, 1680, Centro, CEP 14801-903, SP, Brasil
dc.format.extent2744-2751
dc.identifierhttp://www.amstat.org/meetings/jsm/2013/onlineprogram/AbstractDetails.cfm?abstractid=308949
dc.identifier.citationJSM Proceedings, v. 2013, p. 2744-2751, 2013.
dc.identifier.issn1543-3218
dc.identifier.lattes0127570480681342
dc.identifier.lattes7167211040669505
dc.identifier.urihttp://hdl.handle.net/11449/126097
dc.language.isoeng
dc.relation.ispartofJSM Proceedings
dc.rights.accessRightsAcesso restrito
dc.sourceCurrículo Lattes
dc.subjectIntra-examiner reproducibilityen
dc.subjectIntraclass Correlation Coefficienten
dc.subjectPhospholipase Activityen
dc.subjectCandida albicansen
dc.titleIntra-examiner reproducibility of the agar plate method for estmation of phospholipase activity in candida albicansen
dc.typeArtigo
unesp.author.lattes0127570480681342
unesp.author.lattes7167211040669505
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Odontologia, Araraquarapt

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