Transdentinal diffusion and cytotoxicity of self-etching adhesive systems

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dc.contributor.authorMoreira Lanza, Celia Regina
dc.contributor.authorSouza Costa, Carlos Alberto de [UNESP]
dc.contributor.authorFurlan, Maysa [UNESP]
dc.contributor.authorAlecio, Alberto [UNESP]
dc.contributor.authorHebling, Josimeri [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Federal de Minas Gerais (UFMG)
dc.contributor.institutionPontifícia Universidade Católica de Minas Gerais (PUC-Minas)
dc.date.accessioned2013-09-30T18:31:18Z
dc.date.accessioned2014-05-20T13:44:51Z
dc.date.available2013-09-30T18:31:18Z
dc.date.available2014-05-20T13:44:51Z
dc.date.issued2009-12-01
dc.description.abstractTo evaluated the transdentinal diffusion and subsequent cytotoxicity of self-etching adhesives on odontoblast-like cells.Sixty dentin disks (0.4-mm thick) were produced from human molars and divided into six groups (n = 10). The dentin disks were placed in in vitro pulp chambers where MDPC-23 cells were planted on 0.28 cm(2) of exposed dentin on the pulpal side. The adhesives Clearfil SE Bond (CSE), Clearfil Protect Bond (CPB), Adper Prompt (PR), and Xeno III (XE) were applied on the occlusal side. Single Bond (SB) was used as positive and phosphate buffer solution (PBS) as negative control. The cytotoxicity was measured by MTT assay and cell characteristics were assessed by SEM. The transdentinal diffusion was qualified by GC/MS.Kruskal-Wallis and Mann-Whitney tests demonstrated a significant difference among the adhesives and PBS. Cellular viability reduction promoted by the self-etching systems was lower than that of SB (53.1%), except for CSE. Cell metabolism was reduced in 47.8%, 42.1%, 28.0%, and 46.5% for CSE, CPB, PR, and XE, respectively. HEMA was identified as the main diffused component.Components from all investigated self-etching adhesive systems were able to diffuse through the dentin resulting in significant reduction of the cellular metabolism.en
dc.description.affiliationSão Paulo State Univ UNESP, Dept Orthodont & Pediat Dent, Araraquara Sch Dent, BR-14801903 Araraquara, SP, Brazil
dc.description.affiliationUniversidade Federal de Minas Gerais (UFMG), Dept Oral Clin Surg & Pathol, Sch Dent, Belo Horizonte, MG, Brazil
dc.description.affiliationSão Paulo State Univ UNESP, Dept Physiol & Pathol, Araraquara Sch Dent, BR-14801903 Araraquara, SP, Brazil
dc.description.affiliationSão Paulo State Univ UNESP, Dept Organ Chem, Araraquara Inst Chem, BR-14801903 Araraquara, SP, Brazil
dc.description.affiliationPontificia Univ Catolica Minas Gerais, Dept Pediat Dent, Sch Dent, Belo Horizonte, MG, Brazil
dc.description.affiliationUnespSão Paulo State Univ UNESP, Dept Orthodont & Pediat Dent, Araraquara Sch Dent, BR-14801903 Araraquara, SP, Brazil
dc.description.affiliationUnespSão Paulo State Univ UNESP, Dept Physiol & Pathol, Araraquara Sch Dent, BR-14801903 Araraquara, SP, Brazil
dc.description.affiliationUnespSão Paulo State Univ UNESP, Dept Organ Chem, Araraquara Inst Chem, BR-14801903 Araraquara, SP, Brazil
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdCNPq: 475134/2004-4
dc.description.sponsorshipIdFAPESP: 06/53906-3
dc.format.extent533-543
dc.identifierhttp://dx.doi.org/10.1007/s10565-008-9110-x
dc.identifier.citationCell Biology and Toxicology. Dordrecht: Springer, v. 25, n. 6, p. 533-543, 2009.
dc.identifier.doi10.1007/s10565-008-9110-x
dc.identifier.issn0742-2091
dc.identifier.lattes1308042794786872
dc.identifier.urihttp://hdl.handle.net/11449/15737
dc.identifier.wosWOS:000271482200002
dc.language.isoeng
dc.publisherSpringer
dc.relation.ispartofCell Biology and Toxicology
dc.relation.ispartofjcr3.390
dc.relation.ispartofsjr0,924
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectCell cultureen
dc.subjectCytotoxicityen
dc.subjectDentinen
dc.subjectDentin-bonding agentsen
dc.subjectOdontoblastsen
dc.titleTransdentinal diffusion and cytotoxicity of self-etching adhesive systemsen
dc.typeArtigo
dcterms.licensehttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dcterms.rightsHolderSpringer
unesp.author.lattes1308042794786872
unesp.author.lattes4517484241515548[2]
unesp.author.orcid0000-0002-7455-6867[2]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Química, Araraquarapt
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Odontologia, Araraquarapt

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Artigos - Clínica Infantil - FOAR
Artigos - Fisiologia e Patologia - FOAR
Artigos - Química Orgânica - IQ