Microplate alamarblue assay for paracoccidioides susceptibility testing

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dc.contributor.authorde Paula e Silva, A. C. A. [UNESP]
dc.contributor.authorOliveira, H. C. [UNESP]
dc.contributor.authorSilva, J. F. [UNESP]
dc.contributor.authorSangalli-Leite, F. [UNESP]
dc.contributor.authorScorzoni, L. [UNESP]
dc.contributor.authorFusco-Almeida, A. M. [UNESP]
dc.contributor.authorMendes-Giannini, Maria José Soares [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-27T11:28:46Z
dc.date.available2014-05-27T11:28:46Z
dc.date.issued2013-04-01
dc.description.abstractCLSI method M27-A3 is not available for use with dimorphic fungi, such as those of the Paracoccidioides genus. In this study, we developed a microdilution method and added the alamarBlue reagent to test the responses of Paracoccidioides brasiliensis and Paracoccidioides lutzii against amphotericin B and itraconazole antifungals. The test proved to be sensitive, practical, and inexpensive and can be used to monitor the activity of low-growth microorganisms and their response to various drugs. © 2013, American Society for Microbiology.en
dc.description.affiliationFaculdade de Ciěncias Farmacěuticas UNESP-Universidade Estadual Paulista Departamento de Analises Clinicas, São Paulo
dc.description.affiliationUnespFaculdade de Ciěncias Farmacěuticas UNESP-Universidade Estadual Paulista Departamento de Analises Clinicas, São Paulo
dc.format.extent1250-1252
dc.identifierhttp://dx.doi.org/10.1128/JCM.02914-12
dc.identifier.citationJournal of Clinical Microbiology, v. 51, n. 4, p. 1250-1252, 2013.
dc.identifier.doi10.1128/JCM.02914-12
dc.identifier.file2-s2.0-84875846563.pdf
dc.identifier.issn0095-1137
dc.identifier.issn1098-660X
dc.identifier.orcid0000-0002-8059-0826
dc.identifier.scopus2-s2.0-84875846563
dc.identifier.urihttp://hdl.handle.net/11449/74985
dc.identifier.wosWOS:000316220400032
dc.language.isoeng
dc.relation.ispartofJournal of Clinical Microbiology
dc.relation.ispartofjcr4.054
dc.relation.ispartofsjr2,256
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectalamarBlue
dc.subjectamphotericin B
dc.subjectitraconazole
dc.subjectreagent
dc.subjectunclassified drug
dc.subjectantifungal activity
dc.subjectantifungal susceptibility
dc.subjectbroth dilution
dc.subjectcontrolled study
dc.subjectfungal strain
dc.subjecthuman
dc.subjectintermethod comparison
dc.subjectminimum inhibitory concentration
dc.subjectnonhuman
dc.subjectParacoccidioides
dc.subjectParacoccidioides brasiliensis
dc.subjectParacoccidioides lutzii
dc.subjectpriority journal
dc.subjectAmphotericin B
dc.subjectAntifungal Agents
dc.subjectHumans
dc.subjectItraconazole
dc.subjectMicrobial Sensitivity Tests
dc.subjectOxazines
dc.subjectStaining and Labeling
dc.subjectXanthenes
dc.titleMicroplate alamarblue assay for paracoccidioides susceptibility testingen
dc.typeArtigo
dcterms.licensehttp://journals.asm.org/site/misc/ASM_Author_Statement.xhtml
unesp.author.lattes3716273524139678[6]
unesp.author.orcid0000-0002-8059-0826[7]
unesp.author.orcid0000-0002-2115-8988[6]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Farmacêuticas, Araraquarapt

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Artigos - Análises Clínicas - FCFAR