Requirement for cobalamin by Salmonella enterica serovars Typhimurium, Pullorum, Gallinarum and Enteritidis during infection in chickens

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dc.contributor.authorPaiva, Jacqueline Boldrin de [UNESP]
dc.contributor.authorPenha Filho, Rafael Antonio Casarin [UNESP]
dc.contributor.authorBerchieri Junior, Angelo [UNESP]
dc.contributor.authorLemos, Manoel Victor Franco [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T13:15:54Z
dc.date.available2014-05-20T13:15:54Z
dc.date.issued2011-12-01
dc.description.abstractSalmonella enterica serovar Typhimurium synthesizes cobalamin (vitamin B12) only during anaerobiosis. Two percent of the S. Typhimurium genome is devoted to the synthesis and uptake of vitamin B12 and to B12-dependent reactions. To understand the requirement for cobalamin synthesis better, we constructed mutants of Salmonella serovars Enteritidis and Pullorum that are double-defective in cobalamin biosynthesis (ΔcobSΔcbiA). We compared the virulence of these mutants to that of their respective wild type strains and found no impairment in their ability to cause disease in chickens. We then assessed B12 production in these mutants and their respective wild type strains, as well as in S. Typhimurium ΔcobSΔcbiA, Salmonella Gallinarum ΔcobSΔcbiA, and their respective wild type strains. None of the mutants was able to produce detectable B12. B12 was detectable in S. Enteritidis, S. Pullorum and S. Typhimurium wild type strains but not in S. Gallinarum. In conclusion, the production of vitamin B12 in vitro differed across the tested Salmonella serotypes and the deletion of the cbiA and cobS genes resulted in different levels of alteration in the host parasite interaction according to Salmonella serotype tested.en
dc.description.affiliationUniversidade Estadual Paulista Faculdade de Ciências Agrárias e Veterinárias
dc.description.affiliationUnespUniversidade Estadual Paulista Faculdade de Ciências Agrárias e Veterinárias
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.format.extent1409-1419
dc.identifierhttp://dx.doi.org/10.1590/S1517-83822011000400024
dc.identifier.citationBrazilian Journal of Microbiology. Sociedade Brasileira de Microbiologia, v. 42, n. 4, p. 1409-1419, 2011.
dc.identifier.doi10.1590/S1517-83822011000400024
dc.identifier.fileS1517-83822011000400024.pdf
dc.identifier.issn1517-8382
dc.identifier.lattes3508096260678286
dc.identifier.lattes7179273060624761
dc.identifier.scieloS1517-83822011000400024
dc.identifier.urihttp://hdl.handle.net/11449/2918
dc.identifier.wosWOS:000300430600024
dc.language.isoeng
dc.publisherSociedade Brasileira de Microbiologia
dc.relation.ispartofBrazilian Journal of Microbiology
dc.relation.ispartofjcr1.810
dc.relation.ispartofsjr0,630
dc.rights.accessRightsAcesso aberto
dc.sourceSciELO
dc.subjectSalmonellaen
dc.subjectCobalaminen
dc.subjectgene deletionen
dc.subjectChickensen
dc.titleRequirement for cobalamin by Salmonella enterica serovars Typhimurium, Pullorum, Gallinarum and Enteritidis during infection in chickensen
dc.typeArtigo
dcterms.licensehttp://www.scielo.br/revistas/bjm/paboutj.htm
unesp.author.lattes3508096260678286[3]
unesp.author.lattes7179273060624761
unesp.author.orcid0000-0002-4844-0895[1]
unesp.author.orcid0000-0002-8257-1936[2]
unesp.author.orcid0000-0003-2522-6500[3]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Agrárias e Veterinárias, Jaboticabalpt

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