Histological and immunohistochemical study of the expression of p53 and ki-67 proteins in the mucosa of the tongue, pharynx and larynx of rats exposed to cigarette smoke

Página do item simplificado
dc.contributor.authorSemenzati, Graziela de Oliveira [UNESP]
dc.contributor.authorSalgado, Breno de Souza [UNESP]
dc.contributor.authorRocha, Noeme Sousa [UNESP]
dc.contributor.authorMatheus, Selma Maria Michelin [UNESP]
dc.contributor.authorde Carvalho, Lidia Raquel [UNESP]
dc.contributor.authorGarcia Martins, Regina Helena [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T13:36:38Z
dc.date.available2014-05-20T13:36:38Z
dc.date.issued2012-09-01
dc.description.abstractIntroduction: Head and neck cancers are linked to smoking. The most affected sites are the oral cavity, pharynx and larynx. Experimental studies show epithelial lesions caused by cigarette smoke. Objectives: To investigate in rats the effects of acute cigarette smoke exposure on the mucosa of the tongue, pharynx and larynx.Material and method: Wistar rats were allocated into two groups of 20 animals: CG (control) receiving food and water ad libitum and TG (Tobacco) exposed to the smoke of 40 cigarettes/day for 60 days. Biopsy of their tongues, pharynxes and larynxes were subjected to histopathological, histomorphometric and immunohistochemical studies of protein p53 and ki-67.Result: The histological analysis of tongue from the Tobacco group revealed epithelial hyperplasia (90%), basal cell hyperplasia (95%) and mild to moderate dysplasia (85%). In pharynx showed basal cell hyperplasia (85%), dysplasia (25%) and vascular congestion (95%). In larynx showed basal cell hyperplasia (70%), epithelial hyperplasia (55%), congestion (100%) and inflammatory infiltrate (25%). Morphometric analysis revealed that keratin layer thickness was greater in the tobacco group. P53 immunoexpression was negative in both groups. Ki-67 immunoexpression was positive in basal cell nuclei but in parabasal cell nuclei it was positive only in the Tobacco group.Conclusions: The exposure of animals to cigarette smoke for 60 days resulted in benign lesions. The duration of exposure was not enough to cause the development cancer, as confirmed by the negative expression of p53 protein in all slides examined. Analysis of ki-67 expression showed intense epithelial proliferation in response to damage.en
dc.description.affiliationSão Paulo State Univ UNESP, Disciplina Otorrinolaringol, Dept Oftalmol Otorrinolaringol & Cirurgia Cabeca, Botucatu Med Sch,Fac Med Botucatu, BR-18618970 Botucatu, SP, Brazil
dc.description.affiliationSão Paulo State Univ UNESP, Disciplina Otorrinolaringol, Student Botucatu Med Sch, BR-18618970 Botucatu, SP, Brazil
dc.description.affiliationSão Paulo State Univ UNESP, Student Sch Vet Med & Anim Sci, BR-18618970 Botucatu, SP, Brazil
dc.description.affiliationSão Paulo State Univ UNESP, Dept Pathol, Sch Vet Med & Anim Sci, BR-18618970 Botucatu, SP, Brazil
dc.description.affiliationSão Paulo State Univ UNESP, Botucatu Med Sch, Dept Anat, Inst Biosci, BR-18618970 Botucatu, SP, Brazil
dc.description.affiliationSão Paulo State Univ UNESP, Dept Biostat, Inst Biosci, Botucatu Med Sch, BR-18618970 Botucatu, SP, Brazil
dc.description.affiliationUnespSão Paulo State Univ UNESP, Disciplina Otorrinolaringol, Dept Oftalmol Otorrinolaringol & Cirurgia Cabeca, Botucatu Med Sch,Fac Med Botucatu, BR-18618970 Botucatu, SP, Brazil
dc.description.affiliationUnespSão Paulo State Univ UNESP, Disciplina Otorrinolaringol, Student Botucatu Med Sch, BR-18618970 Botucatu, SP, Brazil
dc.description.affiliationUnespSão Paulo State Univ UNESP, Student Sch Vet Med & Anim Sci, BR-18618970 Botucatu, SP, Brazil
dc.description.affiliationUnespSão Paulo State Univ UNESP, Dept Pathol, Sch Vet Med & Anim Sci, BR-18618970 Botucatu, SP, Brazil
dc.description.affiliationUnespSão Paulo State Univ UNESP, Botucatu Med Sch, Dept Anat, Inst Biosci, BR-18618970 Botucatu, SP, Brazil
dc.description.affiliationUnespSão Paulo State Univ UNESP, Dept Biostat, Inst Biosci, Botucatu Med Sch, BR-18618970 Botucatu, SP, Brazil
dc.format.extent723-731
dc.identifierhttp://dx.doi.org/10.3109/08958378.2012.715317
dc.identifier.citationInhalation Toxicology. London: Informa Healthcare, v. 24, n. 11, p. 723-731, 2012.
dc.identifier.doi10.3109/08958378.2012.715317
dc.identifier.issn0895-8378
dc.identifier.lattes6507858203899415
dc.identifier.lattes8683290712226765
dc.identifier.lattes8683290712226765
dc.identifier.lattes6507858203899415
dc.identifier.lattes8683290712226765
dc.identifier.lattes6077735918469284
dc.identifier.orcid0000-0002-8188-8149
dc.identifier.urihttp://hdl.handle.net/11449/12598
dc.identifier.wosWOS:000308423400003
dc.language.isoeng
dc.publisherInforma Healthcare
dc.relation.ispartofInhalation Toxicology
dc.relation.ispartofjcr1.819
dc.relation.ispartofsjr0,814
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectAirwaysen
dc.subjectexperimental studyen
dc.subjecthistopathologicalen
dc.subjectki-67en
dc.subjectp53en
dc.subjectratsen
dc.subjecttobaccoen
dc.titleHistological and immunohistochemical study of the expression of p53 and ki-67 proteins in the mucosa of the tongue, pharynx and larynx of rats exposed to cigarette smokeen
dc.typeArtigo
dcterms.licensehttp://informahealthcare.com/page/resources/authors
dcterms.rightsHolderInforma Healthcare
unesp.author.lattes8683290712226765
unesp.author.lattes6507858203899415
unesp.author.lattes8683290712226765
unesp.author.lattes6077735918469284
unesp.author.orcid0000-0003-0575-2263[5]
unesp.author.orcid0000-0002-8188-8149
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina, Botucatupt

Arquivos

Licença do Pacote
Agora exibindo 1 - 2 de 2
Nenhuma Miniatura disponível
Nome:
license.txt
Tamanho:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descrição:
Nenhuma Miniatura disponível
Nome:
license.txt
Tamanho:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descrição:

Coleções

Artigos - Oftalmo/Otorrino e CCP - FMB
Artigos - Anatomia - IBB
Artigos - Bioestatística - IBB
Artigos - Patologia - FMB