Uncaria tomentosa Aqueous-ethanol Extract Triggers an Immunomodulation toward a Th2 Cytokine Profile

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dc.contributor.authorDomingues, Alexandre [UNESP]
dc.contributor.authorSartori, Alexandrina [UNESP]
dc.contributor.authorMarino Valente, Ligia Maria
dc.contributor.authorGolim, Márjorie de Assis [UNESP]
dc.contributor.authorSiani, Antonio Carlos
dc.contributor.authorViero, Rosa Marlene [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Federal do Rio de Janeiro (UFRJ)
dc.contributor.institutionFiocruz MS
dc.date.accessioned2014-05-20T13:51:07Z
dc.date.available2014-05-20T13:51:07Z
dc.date.issued2011-08-01
dc.description.abstractUncaria tomentosa (Willd.) DC (Rubiaceae) is a large woody vine that is native to the Amazon and Central American rainforests and is used widely in traditional medicine for its immunomodulatory and antiinflammatory activities. The present work used in vivo immunotoxic and in vitro immunomodulatory experiments to investigate the effects of a pentacyclic oxindole alkaloid extract from U. tomentosa bark on lymphocyte phenotype, Th1/Th2 cytokine production, cellular proliferation and cytotoxicity. For the in vivo immunotoxicity testing, BALB/c male mice were treated once a day with 125, 500 or 1250mg/kg of U. tomentosa extract for 28 days. For the in vitro protocol, lymphocytes were cultured with 10-500 mu g/mg of the extract for 48 h. The extract increased the cellularity of splenic white pulp and the thymic medulla and increased the number of T helper lymphocytes and B lymphocytes. Also, a large stimulatory effect on lymphocyte viability was observed. However, mitogen-induced T lymphocyte proliferation was significantly inhibited at higher concentrations of U. tomentosa extract. Furthermore, an immunological polarization toward a Th2 cytokine profile was observed. These results suggest that the U. tomentosa aqueous-ethanol extract was not immunotoxic to mice and was able to modulate distinct patterns of the immune system in a dose-dependent manner. Copyright (C) 2011 John Wiley & Sons, Ltd.en
dc.description.affiliationUniv Estadual Paulista, Sch Med, Dept Pathol, BR-18618000 São Paulo, Brazil
dc.description.affiliationUniv Estadual Paulista, Biosci Inst, Dept Microbiol & Immunol, BR-18618000 São Paulo, Brazil
dc.description.affiliationUniv Fed Rio de Janeiro, Inst Chem, Dept Organ Chem, BR-21941909 Rio de Janeiro, Brazil
dc.description.affiliationUniv Estadual Paulista, Sch Med, Ctr Hematol, BR-18618000 São Paulo, Brazil
dc.description.affiliationFiocruz MS, Oswaldo Cruz Fdn, Lab Nat Prod, Inst Pharmaceut Technol, BR-21041250 Rio de Janeiro, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Sch Med, Dept Pathol, BR-18618000 São Paulo, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Biosci Inst, Dept Microbiol & Immunol, BR-18618000 São Paulo, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Sch Med, Ctr Hematol, BR-18618000 São Paulo, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipIdFAPESP: 08/53113-9
dc.format.extent1229-1235
dc.identifierhttp://dx.doi.org/10.1002/ptr.3549
dc.identifier.citationPhytotherapy Research. Malden: Wiley-blackwell, v. 25, n. 8, p. 1229-1235, 2011.
dc.identifier.doi10.1002/ptr.3549
dc.identifier.issn0951-418X
dc.identifier.lattes4977572416129527
dc.identifier.lattes1453564171333848
dc.identifier.urihttp://hdl.handle.net/11449/18256
dc.identifier.wosWOS:000293970400019
dc.language.isoeng
dc.publisherWiley-Blackwell
dc.relation.ispartofPhytotherapy Research
dc.relation.ispartofjcr3.349
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectUncaria tomentosaen
dc.subjectimmune systemen
dc.subjectmiceen
dc.subjectimmunotoxicityen
dc.subjectcytokinesen
dc.subjectimmunophenotypeen
dc.titleUncaria tomentosa Aqueous-ethanol Extract Triggers an Immunomodulation toward a Th2 Cytokine Profileen
dc.typeArtigo
dcterms.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dcterms.rightsHolderWiley-blackwell
unesp.author.lattes4977572416129527[2]
unesp.author.lattes1453564171333848
unesp.author.orcid0000-0003-4557-3331[2]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina, Botucatupt

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Artigos - Microbiologia e Imunologia - IBB
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