New seminal plasma removal method for freezing stallion semen

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dc.contributor.authorRamires Neto, C. [UNESP]
dc.contributor.authorMonteiro, G. A. [UNESP]
dc.contributor.authorSoares, R. F. [UNESP]
dc.contributor.authorPedrazzi, C.
dc.contributor.authorDell'aqua Junior, José Antônio [UNESP]
dc.contributor.authorPapa, Frederico Ozanam [UNESP]
dc.contributor.authorCastro-Chaves, M. M. [UNESP]
dc.contributor.authorAlvarenga, Marco Antonio [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionCEAFEPE Tecnologia Veterinária Ltd.
dc.date.accessioned2014-05-27T11:28:55Z
dc.date.available2014-05-27T11:28:55Z
dc.date.issued2013-04-15
dc.description.abstractSeminal plasma removal, an indispensable step in equine semen cryopreservation, is usually done by centrifugation, but this might cause mechanical damage to sperm. A new method for seminal plasma removal from stallion semen, namely a filter composed of a synthetic hydrophilic membrane (Sperm Filter, BotuPharma, Botucatu, Sao Paulo, Brazil), was recently proposed. The objective of this study was to test the use of the Sperm Filter in the removal of seminal plasma before freezing stallion semen. Ejaculates from 31 stallions were divided into two groups and cryopreserved. In group 1 (G1), seminal plasma was removed with the Sperm Filter, and in group 2 (G2), seminal plasma was removed by centrifugation (600× g for 10 minutes). There were no differences (P < 0.05) between G1 and G2 in sperm kinetic parameters or plasma membrane integrity before or after cryopreservation. However, sperm recovery rate was higher (P < 0.05) for G1 versus G2 (mean ± SD, 89.4 ± 7.4% vs. 80.9 ± 5.5%). Therefore, the Sperm Filter was as efficient as centrifugation in removing seminal plasma from the stallion ejaculate. However, filtering was more practical and had significantly fewer sperm lost than the centrifugation technique. © 2013 Elsevier Inc.en
dc.description.affiliationCollege of Veterinary Medicine and Animal Science - (UNESP) Univ Estadual Paulista Department of Animal Reproduction and Veterinary Radiology, Botucatu, Sao Paulo
dc.description.affiliationCEAFEPE Tecnologia Veterinária Ltd., Sorocaba, Sao Paulo
dc.description.affiliationUnespCollege of Veterinary Medicine and Animal Science - (UNESP) Univ Estadual Paulista Department of Animal Reproduction and Veterinary Radiology, Botucatu, Sao Paulo
dc.format.extent1120-1123
dc.identifierhttp://dx.doi.org/10.1016/j.theriogenology.2013.01.014
dc.identifier.citationTheriogenology, v. 79, n. 7, p. 1120-1123, 2013.
dc.identifier.doi10.1016/j.theriogenology.2013.01.014
dc.identifier.issn0093-691X
dc.identifier.lattes0640158639112242
dc.identifier.lattes0473846154288947
dc.identifier.scopus2-s2.0-84878081267
dc.identifier.urihttp://hdl.handle.net/11449/75114
dc.identifier.wosWOS:000317325300013
dc.language.isoeng
dc.relation.ispartofTheriogenology
dc.relation.ispartofjcr2.136
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectCentrifugation
dc.subjectHorse
dc.subjectSemen
dc.subjectSeminal plasma
dc.subjectSperm filter
dc.subjectStallion
dc.titleNew seminal plasma removal method for freezing stallion semenen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
unesp.author.lattes0640158639112242
unesp.author.lattes0473846154288947
unesp.author.orcid0000-0002-5858-8535[6]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina Veterinária e Zootecnia, Botucatupt

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