Taenia saginata: Differential diagnosis of human taeniasis by polymerase chain reaction-restriction fragment length polymorphism assay

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dc.contributor.authorNunes, Caris Maroni [UNESP]
dc.contributor.authorDias, AKK
dc.contributor.authorDias, FEF
dc.contributor.authorAoki, S. M.
dc.contributor.authorde Paula, H. B.
dc.contributor.authorLima, LGF
dc.contributor.authorGarcia, José Fernando [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-02-26T17:22:35Z
dc.date.accessioned2014-05-20T13:42:19Z
dc.date.available2014-02-26T17:22:35Z
dc.date.available2014-05-20T13:42:19Z
dc.date.issued2005-08-01
dc.description.abstractSpeciation of Taenia in human stool is important because of their different clinical and epidemiological features. DNA analysis has recently become possible which overcomes the problems of differentiating human taeniid cestodes morphologically. In the present study, we evaluated PCR coupled to restriction fragment length polymorphism to differentiate Taenia solium from Taenia saginata eggs present in fecal samples from naturally infected patients. A different Dral-RFLP pattern: a two-band pattern (421 and 100 bp) for T saginata and a three-band pattern (234, 188, and 99 bp) for T solium was observed allowing the two species to be separated.. The lower detection limit of the PCR-RFLP using a non-infected fecal sample prepared with a given number of T saginata eggs was 34 eggs in 2 g stool sediment. The 521 bp mtDNA fragment was detected in 8 out of 12 Taenia sp. carriers (66.6%). of these, three showed a T solium pattern and five a T saginata pattern. (c) 2005 Elsevier B.V. All rights reserved.en
dc.description.affiliationUNESP, Dept Anim Hlth & Prod, BR-16050680 Aracatuba, SP, Brazil
dc.description.affiliationUnespUNESP, Dept Anim Hlth & Prod, BR-16050680 Aracatuba, SP, Brazil
dc.format.extent412-415
dc.identifierhttp://dx.doi.org/10.1016/j.exppara.2005.03.008
dc.identifier.citationExperimental Parasitology. San Diego: Academic Press Inc. Elsevier B.V., v. 110, n. 4, p. 412-415, 2005.
dc.identifier.doi10.1016/j.exppara.2005.03.008
dc.identifier.issn0014-4894
dc.identifier.lattes1892359871207408
dc.identifier.lattes9991374083045897
dc.identifier.urihttp://hdl.handle.net/11449/14709
dc.identifier.wosWOS:000230878600011
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofExperimental Parasitology
dc.relation.ispartofjcr1.821
dc.relation.ispartofsjr0,635
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjecttaeniasispt
dc.subjectPCR-RFLPpt
dc.subjectdiagnosispt
dc.subjectfecal samplespt
dc.subjectTaemia saginatapt
dc.subjectTaenia soliumpt
dc.titleTaenia saginata: Differential diagnosis of human taeniasis by polymerase chain reaction-restriction fragment length polymorphism assayen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.
unesp.author.lattes1892359871207408[1]
unesp.author.lattes9991374083045897
unesp.author.orcid0000-0002-5463-3845[1]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina Veterinária, Araçatubapt

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