Healing Actions of Essential Oils from Citrus aurantium and d-Limonene in the Gastric Mucosa: The Roles of VEGF, PCNA, and COX-2 in Cell Proliferation

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dc.contributor.authorMoraes, Thiago Mello [UNESP]
dc.contributor.authorRozza, Ariane Leite [UNESP]
dc.contributor.authorKushima, Helio [UNESP]
dc.contributor.authorPellizzon, Claudia Helena [UNESP]
dc.contributor.authorMachado Rocha, Lucia Regina [UNESP]
dc.contributor.authorHiruma-Lima, Clelia Akiko [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-12-03T13:10:53Z
dc.date.available2014-12-03T13:10:53Z
dc.date.issued2013-12-01
dc.description.abstractPrevious studies have described the gastroprotective effects of essential oils that are derived from Citrus aurantium (OEC) and its main compound d-limonene (LIM) in a model of chemically induced ulcers in rats. However, these studies do not address the compound's healing effects on the gastric mucosa. Thus, the aim of this work was to evaluate the healing activity of OEC and LIM in acetic acid-induced gastric ulcers in rats, a model that reproduces human chronic ulcers. The obtained results demonstrated that lower effective doses of OEC (250mg/kg) and LIM (245mg/kg) induced gastric mucosal healing with a cure rate of 44% and 56%, respectively, compared with the control group (P<.05). During the 14 days of OEC or LIM treatment, none of the groups demonstrated toxicity in terms of body and organ weight or serum biochemical parameters. Both OEC and LIM treatment promoted an increase in epithelial healing, as confirmed by immunohistochemistry, which was greater in the animals that were treated with the positive control. In addition, both treatments increased cellular proliferation as measured by proliferating cell nuclear antigen and cyclooxygenase 2 expression in the gastric mucosa, vascular endothelial growth factor-mediated blood vessel formation in the margin of the ulcer, and production of gastric mucus, which fortifies the gastric protective barrier. We concluded that OEC and LIM, two common flavoring agents, promote gastric mucosal healing without any apparent toxic effect, resulting in better gastric epithelial organization in the treated rats.en
dc.description.affiliationSao Paulo State Univ UNESP, Dept Physiol, Biosci Inst, Botucatu, SP, Brazil
dc.description.affiliationSao Paulo State Univ UNESP, Dept Morphol, Biosci Inst, Botucatu, SP, Brazil
dc.description.affiliationUnespSao Paulo State Univ UNESP, Dept Physiol, Biosci Inst, Botucatu, SP, Brazil
dc.description.affiliationUnespSao Paulo State Univ UNESP, Dept Morphol, Biosci Inst, Botucatu, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.format.extent1162-1167
dc.identifierhttp://dx.doi.org/10.1089/jmf.2012.0259
dc.identifier.citationJournal Of Medicinal Food. New Rochelle: Mary Ann Liebert, Inc, v. 16, n. 12, p. 1162-1167, 2013.
dc.identifier.doi10.1089/jmf.2012.0259
dc.identifier.issn1096-620X
dc.identifier.lattes0019393779801069
dc.identifier.lattes3814504901386844
dc.identifier.orcid0000-0002-8645-3777
dc.identifier.orcid0000-0002-4494-4180
dc.identifier.urihttp://hdl.handle.net/11449/112624
dc.identifier.wosWOS:000328457600013
dc.language.isoeng
dc.publisherMary Ann Liebert, Inc.
dc.relation.ispartofJournal of Medicinal Food
dc.relation.ispartofjcr1.954
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectCitrus aurantiumen
dc.subjectessential oilen
dc.subjectd-limoneneen
dc.subjecthealing ulceren
dc.titleHealing Actions of Essential Oils from Citrus aurantium and d-Limonene in the Gastric Mucosa: The Roles of VEGF, PCNA, and COX-2 in Cell Proliferationen
dc.typeArtigo
dcterms.rightsHolderMary Ann Liebert, Inc
unesp.author.lattes2328604224911233[2]
unesp.author.lattes0019393779801069[4]
unesp.author.lattes3814504901386844[6]
unesp.author.orcid0000-0002-4198-0135[2]
unesp.author.orcid0000-0002-8645-3777[6]
unesp.author.orcid0000-0002-4494-4180[4]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt

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