Expression of a new Bacillus thuringiensis Cry1Ia gene in Escherichia coli with strong activity against cotton pests

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dc.contributor.authorBergamasco, V. B. [UNESP]
dc.contributor.authorGonçalves, J. F. [UNESP]
dc.contributor.authorPolanczyk, R. A. [UNESP]
dc.contributor.authorDesidério, J. A. [UNESP]
dc.contributor.authorLemos, Manoel Victor Franco [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-27T11:26:12Z
dc.date.available2014-05-27T11:26:12Z
dc.date.issued2011-12-01
dc.description.abstractThe control of cotton pests may be accomplished using Bacillus thuringiensis Cry proteins. For this purpose, the objective of this work was to evaluate the insecticidal activity of a new Cry1Ia protein against neonatal larvae of Spodoptera frugiperda and Anthonomus grandis. The complete cry1Ia gene, previously obtained by PCR with oligonucleotide primers based on the sequenced gene, was cloned into the vector pET28a(+), introduced into Escherichia coli BL21(DE3) and expressed by induction with IPTG. The expression of the Cry1Ia protein was confirmed with molecular weight of approximately 81 kDa. The results demonstrated the efficiency of the bacterial system for the expression of B. thuringiensis Cry1Ia protein, which was subsequently used in quantitative bioassays against S. frugiperda and A. grandis larvae, resulting in an extremely toxic protein for both species. This characteristic is exceptionally important for obtaining transgenic cotton plants resistant to these pests.en
dc.description.affiliationDepartment of Biology Applied to Agriculture Faculty of Agronomic and Veterinary Science-FCAV São Paulo State University-UNESP, Via de Acesso Prof. Paulo Donato Castellane, s/n, CEP 14884-900, Jaboticabal, SP
dc.description.affiliationDepartment of Fitossanity Faculty of Agronomic and Veterinary Science-FCAV São Paulo State University-UNESP, Via de Acesso Prof. Paulo Donato Castellane, s/n, CEP 14884-900, Jaboticabal, SP
dc.description.affiliationUnespDepartment of Biology Applied to Agriculture Faculty of Agronomic and Veterinary Science-FCAV São Paulo State University-UNESP, Via de Acesso Prof. Paulo Donato Castellane, s/n, CEP 14884-900, Jaboticabal, SP
dc.description.affiliationUnespDepartment of Fitossanity Faculty of Agronomic and Veterinary Science-FCAV São Paulo State University-UNESP, Via de Acesso Prof. Paulo Donato Castellane, s/n, CEP 14884-900, Jaboticabal, SP
dc.format.extent526-533
dc.identifierhttp://connection.ebscohost.com/c/articles/74249032
dc.identifier.citationAustralian Journal of Basic and Applied Sciences, v. 5, n. 12, p. 526-533, 2011.
dc.identifier.issn1991-8178
dc.identifier.lattes2788156792532870
dc.identifier.lattes1295051340591836
dc.identifier.lattes7179273060624761
dc.identifier.orcid0000-0003-0769-9902
dc.identifier.scopus2-s2.0-84856455763
dc.identifier.urihttp://hdl.handle.net/11449/72827
dc.language.isoeng
dc.relation.ispartofAustralian Journal of Basic and Applied Sciences
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectAnthonomus grandis
dc.subjectColeoptera
dc.subjectCry Genes
dc.subjectLepidoptera
dc.subjectSpodoptera frugiperda
dc.titleExpression of a new Bacillus thuringiensis Cry1Ia gene in Escherichia coli with strong activity against cotton pestsen
dc.typeArtigo
unesp.author.lattes1295051340591836
unesp.author.lattes7179273060624761
unesp.author.lattes2788156792532870[3]
unesp.author.orcid0000-0003-0769-9902[3]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Agrárias e Veterinárias, Jaboticabalpt

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Artigos - Biologia - FCAV
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