Potential utility of melatonin as an antioxidant therapy in the management of sickle cell anemia

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dc.contributor.authorSilva, Danilo Grünig Humberto da [UNESP]
dc.contributor.authorRicci Júnior, Octávio
dc.contributor.authorAlmeida, Eduardo Alves de [UNESP]
dc.contributor.authorBonini-Domingos, Claudia Regina [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionFaculdade de Medicina de São José do Rio Preto (FAMERP)
dc.date.accessioned2015-04-27T11:56:07Z
dc.date.available2015-04-27T11:56:07Z
dc.date.issued2014
dc.description.abstractThis study aimed to assess antioxidant effects of melatonintreatment compared to N-acetylcysteine (NAC) and to their combination in asickle cell suspension. Sickle erythrocytes were suspended in phosphate-buffered saline, pH 7.4, composing external control group. They were alsosuspended and incubated at 37°C either in the absence (experimental controlgroup) or in the presence of NAC, melatonin and their combination atconcentrations of 100 pM, 100 nM and 100 lM for 1 hr (treatment groups).The melatonin influences were evaluated by spectrophotometric [hemolysisdegree, catalase (CAT), glutathione S-transferase (GST), glutathioneperoxidase (GPx), glutathione reductase (GR), glucose-6-phosphatedehydrogenase (G6PDH), and superoxide dismutase (SOD) activities] andchromatographic methods [glutathione (GSH) and malondialdehyde (MDA)levels]. Incubation period was able to cause a rise about 64% on hemolysisdegree as well as practically doubled the lipid peroxidation levels (P < 0.01).However, almost all antioxidants tested treatments neutralized this incubationeffect observed in MDA levels. Among the antioxidant biomarkers evaluated,we observed a modulating effect of combined treatment on GPx and SODactivities (P < 0.01), which showed ~25% decrease in their activities. Inaddition, we found an antioxidant dose-dependent effect for melatonin onlipid peroxidation (r = 0.29; P = 0.03) and for combined antioxidanttreatments also on MDA levels (r = 0.37; P = 0.01) and on SOD activity(r = 0.54; P < 0.01). Hence, these findings contribute with important insightthat melatonin individually or in combination with NAC may be useful forsickle cell anemia management.en
dc.description.affiliationUniversidade Estadual Paulista Júlio de Mesquita Filho, Departamento de Biologia, Instituto de Biociências Letras e Ciências Exatas de São José do Rio Preto, Sao Jose do Rio Preto, Rua Cristóvão Colombo, 2265, Jardim Nazareth, CEP 15054-000, SP, Brasil
dc.description.affiliationUnespUniversidade Estadual Paulista Júlio de Mesquita Filho, Departamento de Biologia, Instituto de Biociências Letras e Ciências Exatas de São José do Rio Preto
dc.description.affiliationUnespUniversidade Estadual Paulista Júlio de Mesquita Filho, Departamento de Química e Ciências Ambientais, Instituto de Biociências Letras e Ciências Exatas de São José do Rio Preto
dc.format.extent178-188
dc.identifierhttp://onlinelibrary.wiley.com/doi/10.1111/jpi.12204/abstract
dc.identifier.citationJournal of Pineal Research, v. 58, n. 2, p. 178-188, 2014.
dc.identifier.doi10.1111/jpi.12204
dc.identifier.issn0742-3098
dc.identifier.lattes6713400866382255
dc.identifier.lattes3279428066176719
dc.identifier.orcid0000-0002-4603-9467
dc.identifier.urihttp://hdl.handle.net/11449/122879
dc.language.isoeng
dc.relation.ispartofJournal of Pineal Research
dc.relation.ispartofjcr11.613
dc.relation.ispartofsjr3,945
dc.rights.accessRightsAcesso restrito
dc.sourceCurrículo Lattes
dc.subjectalternative therapyen
dc.subjectantioxidant capacityen
dc.subjecthemoglobin Sen
dc.subjectmelatoninen
dc.subjectoxidative stressen
dc.titlePotential utility of melatonin as an antioxidant therapy in the management of sickle cell anemiaen
dc.typeArtigo
unesp.author.lattes6713400866382255
unesp.author.lattes3279428066176719[4]
unesp.author.orcid0000-0002-4603-9467[4]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências Letras e Ciências Exatas, São José do Rio Pretopt
unesp.departmentBiologiapt

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