Production, purification and characterization of a minor form of xylanase from Aspergillus versicolor

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dc.contributor.authorCarmona, E. C.
dc.contributor.authorFialho, M. B.
dc.contributor.authorBuchgnani, E. B.
dc.contributor.authorCoelho, G. D.
dc.contributor.authorBrocheto-Braga, M. R.
dc.contributor.authorJorge, J. A.
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T13:56:07Z
dc.date.available2014-05-20T13:56:07Z
dc.date.issued2005-01-01
dc.description.abstractA strain of Aspergillus versicolor produces a xylanolytic complex containing two components, the minor component being designated xylanase II. The highest production of xylanase II was observed in cultures grown for 5 days in 1% wheat bran as carbon source, at pH 6.5. Xylanase II was purified 28-fold by DEAE-Sephadex and HPLC GF-5 10 gel filtration. Xylanase II was a monomeric glycoprotein, exhibiting a molecular mass of 32 kDa with 14.1% of carbohydrate content. Optimal pH and temperature values for the enzyme activity were about 6.0-7.0 and 55 degreesC, respectively. Xylanase II thermoinactivation at 50degreesC showed a biphasic curve. The ions Hg2+, Cu2+ and the detergent SDS were strong inhibitors, while Mn2+ ions and dithiothreitol were stimulators of the enzyme activity. The enzyme was specific for xylans, showing higher specific activity on birchwood xylan. The Michaelis-Menten constant (K-m) for birchwood xylan was estimated to be 2.3 mg ml(-1) while maximal velocity (V-max) was 233.1 mumol mg(-1) min(-1) of protein. The hydrolysis of oat spell xylan released only xylooligosaccharides. Published by Elsevier Ltd.en
dc.description.affiliationUNESP, Inst Biociencias, Dept Bioquim & Microbiol, BR-13506900 Rio Claro, SP, Brazil
dc.description.affiliationUnespUNESP, Inst Biociencias, Dept Bioquim & Microbiol, BR-13506900 Rio Claro, SP, Brazil
dc.format.extent359-364
dc.identifierhttp://dx.doi.org/10.1016/j.procbio.2004.01.010
dc.identifier.citationProcess Biochemistry. Oxford: Elsevier B.V., v. 40, n. 1, p. 359-364, 2005.
dc.identifier.doi10.1016/j.procbio.2004.01.010
dc.identifier.issn1359-5113
dc.identifier.lattes4110421764783871
dc.identifier.urihttp://hdl.handle.net/11449/20079
dc.identifier.wosWOS:000224162000047
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofProcess Biochemistry
dc.relation.ispartofjcr2.616
dc.relation.ispartofsjr0,761
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectAspergillus versicolorpt
dc.subjectxylanasept
dc.subjectendoxylanasept
dc.subjectenzyme purificationpt
dc.titleProduction, purification and characterization of a minor form of xylanase from Aspergillus versicoloren
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.
unesp.author.lattes4110421764783871
unesp.author.orcid0000-0002-0230-5735[1]
unesp.author.orcid0000-0002-0378-0730[2]
unesp.author.orcid0000-0002-7982-0743[5]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Rio Claropt

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