Preparation and characterization of a bacterial cellulose/silk fibroin sponge scaffold for tissue regeneration

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dc.contributor.authorOliveira Barud, H. G. [UNESP]
dc.contributor.authorBarud, Hernane da S. [UNESP]
dc.contributor.authorCavicchioli, Maurício [UNESP]
dc.contributor.authorAmaral, Thais Silva do [UNESP]
dc.contributor.authorOliveira Junior, Osmir Batista de [UNESP]
dc.contributor.authorSantos, Diego M.
dc.contributor.authorPetersen, Antonio Luis de Oliveira Almeida
dc.contributor.authorCeles, Fabiana
dc.contributor.authorBorges, Valéria Matos
dc.contributor.authorOliveira, Camila I. de
dc.contributor.authorOliveira, Pollyanna Francielli de
dc.contributor.authorFurtado, Ricardo Andrade
dc.contributor.authorTavares, Denise Crispim
dc.contributor.authorRibeiro, Sidney J. L. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionCentro de Pesquisas Gonçalo Moniz
dc.contributor.institutionUniversidade de Franca
dc.contributor.institutionCentro Universitário de Araraquara (UNIARA)
dc.date.accessioned2015-12-07T15:37:38Z
dc.date.available2015-12-07T15:37:38Z
dc.date.issued2015-09-05
dc.description.abstractBacterial cellulose (BC) and silk fibroin (SF) are natural biopolymers successfully applied in tissue engineering and biomedical fields. In this work nanocomposites based on BC and SF were prepared and characterized by scanning electron microscopy (SEM), infrared spectroscopy (FT-IR), X-ray diffraction (XRD) and thermogravimetric analysis (TGA). In addition, the investigation of cytocompatibility was done by MTT, XTT and Trypan Blue dye technique. Cellular adhesion and proliferation were detected additionally. The evaluation of genotoxicity was realized by micronucleus assay. In vitro tests showed that the material is non-cytotoxic or genotoxic. SEM images revealed a greater number of cells attached at the BC/SF:50% scaffold surface than the pure BC one, suggesting that the presence of fibroin improved cell attachment. This could be related to the SF amino acid sequence that acts as cell receptors facilitating cell adhesion and growth. Consequently, BC/SF:50% scaffolds configured an excellent option in bioengineering depicting its potential for tissue regeneration and cultivation of cells on nanocomposites.en
dc.description.affiliationInsituto de Química (IQ), Universidade Estadual Paulista (UNESP), Araraquara, SP, Brasil
dc.description.affiliationFaculdade de Odontologia de Araraquara (FOAR), Universidade Estadual Paulista (UNESP), Araraquara, SP, Brasil
dc.description.affiliationCentro de Pesquisas Gonçalo Moniz, Fundação Oswaldo Cruz (FIOCRUZ), Salvador, BA, Brasil
dc.description.affiliationUniversidade de Franca (UNIFRA), Franca, SP, Brasil
dc.description.affiliationLaboratório de Química Medicinal e Medicina Regenerativa (QUIMMERA), Centro Universitário de Araraquara (UNIARA), Araraquara, SP, Brasil
dc.description.affiliationUnespInsituto de Química (IQ), Universidade Estadual Paulista (UNESP), Araraquara, SP, Brasil
dc.description.affiliationUnespFaculdade de Odontologia de Araraquara (FOAR), Universidade Estadual Paulista (UNESP), Araraquara, SP, Brasil
dc.format.extent41-51
dc.identifierhttp://dx.doi.org/10.1016/j.carbpol.2015.04.007
dc.identifier.citationCarbohydrate Polymers, v. 128, p. 41-51, 2015.
dc.identifier.doi10.1016/j.carbpol.2015.04.007
dc.identifier.issn1879-1344
dc.identifier.lattes0170241211219616
dc.identifier.orcid0000-0002-2153-9887
dc.identifier.pubmed26005138
dc.identifier.urihttp://hdl.handle.net/11449/131561
dc.language.isoeng
dc.publisherElsevier B. V.
dc.relation.ispartofCarbohydrate Polymers
dc.rights.accessRightsAcesso restrito
dc.sourcePubMed
dc.subjectBacterial celluloseen
dc.subjectSilk fibroinen
dc.subjectBiocompatible materialsen
dc.subjectNanocompositesen
dc.subjectScaffolden
dc.subjectTissue engineeringen
dc.titlePreparation and characterization of a bacterial cellulose/silk fibroin sponge scaffold for tissue regenerationen
dc.typeArtigo
dcterms.rightsHolderElsevier B. V.
unesp.author.lattes0170241211219616[5]
unesp.author.orcid0000-0003-3286-9440[14]
unesp.author.orcid0000-0002-2153-9887[5]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Química, Araraquarapt

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