Antimicrobial analysis of chlorhexidine gel and intracanal medicaments against microorganisms inoculated in root canals

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dc.contributor.authorValera, M. C.
dc.contributor.authorSalvia, A. C.
dc.contributor.authorMaekawa, L. E.
dc.contributor.authorCamargo, S. E.
dc.contributor.authorCarvalho, C. A.
dc.contributor.authorCamargo, C. H.
dc.contributor.authorKoga-Ito, C. Y.
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-27T11:24:44Z
dc.date.available2014-05-27T11:24:44Z
dc.date.issued2010-07-01
dc.description.abstractThe aim of this paper was to evaluate the antimicrobial activity of 2% chlorhexidine gel (CLX) associated with various intracanal medicaments against Candida albicans and Enterococcus faecalis inoculated in root canals. Thirty six human single-rooted teeth were contaminated with C.albicans and E.faecalis. The canals were instrumented using 2% CLX gel and were divided into three groups according to the intracanal medicaments (ICM) used. Group 1: calcium hydroxide paste [Ca(OH)], Group 2: 2% chlorhexidine gel (CLX) and Group 3: 2% CLX gel + Ca(OH). The root canal collections were performed after 21 days of contamination (control collection), after instrumentation (1st collection), after 14 days of intracanal medicament (2nd collection) and 7 days after medicament removal (3rd collection). The microbiological samples were plated in culture media and incubated for 48 hours. The results were submitted to Kruskal-Wallis test (P ≤ 0.05). It was verified that the instrumentation with CLX reduced the number of CFU/ml significantly when compared with the confirmation collection (control). However, the use of the ICM was only capable to eliminate completely the microorganisms in the root canals without difference statistics between them. Although the use of 2% chlorherixidine gel reduces the number of microorganisms significantly, only the ICM calcium hydroxide and calcium hydroxide associated with chlorhexidine are able to eliminate these microorganisms completely.en
dc.format.extent415-421
dc.identifierhttp://www.minervamedica.it/en/journals/minerva-stomatologica/article.php?cod=R18Y2010N07A0415
dc.identifier.citationMinerva stomatologica, v. 59, n. 7-8, p. 415-421, 2010.
dc.identifier.issn0026-4970
dc.identifier.lattes6543563161403421
dc.identifier.orcid0000-0002-2416-2173
dc.identifier.scopus2-s2.0-79952846090
dc.identifier.urihttp://hdl.handle.net/11449/71771
dc.language.isoeng
dc.relation.ispartofMinerva stomatologica
dc.relation.ispartofsjr0,301
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectcalcium hydroxide
dc.subjectchlorhexidine
dc.subjectchlorhexidine gluconate
dc.subjectdrug derivative
dc.subjectbacterial load
dc.subjectCandida albicans
dc.subjectcomparative study
dc.subjectdental equipment
dc.subjectdrug effect
dc.subjectdrug potentiation
dc.subjectEnterococcus faecalis
dc.subjectequipment
dc.subjectevaluation
dc.subjectgel
dc.subjectgrowth, development and aging
dc.subjecthuman
dc.subjectin vitro study
dc.subjectisolation and purification
dc.subjectmicrobiological examination
dc.subjectmicrobiology
dc.subjecttooth pulp
dc.subjectBacterial Load
dc.subjectCalcium Hydroxide
dc.subjectChlorhexidine
dc.subjectDental Instruments
dc.subjectDental Pulp Cavity
dc.subjectDrug Synergism
dc.subjectEquipment Contamination
dc.subjectGels
dc.subjectHumans
dc.subjectMicrobial Sensitivity Tests
dc.titleAntimicrobial analysis of chlorhexidine gel and intracanal medicaments against microorganisms inoculated in root canalsen
dc.typeArtigo
unesp.author.lattes6543563161403421[7]
unesp.author.orcid0000-0001-7398-6438[1]
unesp.author.orcid0000-0002-2416-2173[7]

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