Serotonergic neurons in the nucleus raphe obscurus contribute to interaction between central and peripheral ventilatory responses to hypercapnia

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dc.contributor.authorda Silva, Glauber S. F.
dc.contributor.authorGiusti, Humberto
dc.contributor.authorBenedetti, Mauricio
dc.contributor.authorDias, Mirela B.
dc.contributor.authorGargaglioni, Luciane H. [UNESP]
dc.contributor.authorBranco, Luiz Guilherme S.
dc.contributor.authorGlass, Mogens L.
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Federal de Goiás (UFG)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T13:15:41Z
dc.date.available2014-05-20T13:15:41Z
dc.date.issued2011-09-01
dc.description.abstractSerotonergic (5-HT) neurons in the nucleus raphe obscurus (ROb) are involved in the respiratory control network. However, it is not known whether ROb 5-HT neurons play a role in the functional interdependence between central and peripheral chemoreceptors. Therefore, we investigated the role of ROb 5-HT neurons in the ventilatory responses to CO2 and their putative involvement in the central-peripheral CO2 chemoreceptor interaction in unanaesthetised rats. We used a chemical lesion specific for 5-HT neurons (anti-SERT-SAP) of the ROb in animals with the carotid body (CB) intact or removed (CBR). Pulmonary ventilation (V (E)), body temperature and the arterial blood gases were measured before, during and after a hypercapnic challenge (7% CO2). The lesion of ROb 5-HT neurons alone (CB intact) or the lesion of 5-HT neurons of ROb+CBR did not affect baseline V (E) during normocapnic condition. Killing ROb 5-HT neurons (CB intact) significantly decreased the ventilatory response to hypercapnia (p < 0.05). The reduction in CO2 sensitivity was approximately 15%. When ROb 5-HT neurons lesion was combined with CBR (anti-SERT-SAP+CBR), the V (E) response to hypercapnia was further decreased (-31.2%) compared to the control group. The attenuation of CO2 sensitivity was approximately 30%, and it was more pronounced than the sum of the individual effects of central (ROb lesion; -12.3%) or peripheral (CBR; -5.5%) treatments. Our data indicate that ROb 5-HT neurons play an important role in the CO2 drive to breathing and may act as an important element in the central-peripheral chemoreception interaction to CO2 responsiveness.en
dc.description.affiliationUniv São Paulo USP FMRP, Dept Physiol, BR-14049900 Ribeirao Preto, SP, Brazil
dc.description.affiliationUniversidade Federal de Goiás (UFG), Inst Biol Sci, Dept Physiol Sci, Goiania, Go, Brazil
dc.description.affiliationSão Paulo State Univ UNESP FCAV Jaboticabal, Dept Anim Morphol & Physiol, Jaboticabal, SP, Brazil
dc.description.affiliationUniv São Paulo USP FORP, Dept Morphol Stomatol & Physiol, Ribeirao Preto, SP, Brazil
dc.description.affiliationUnespSão Paulo State Univ UNESP FCAV Jaboticabal, Dept Anim Morphol & Physiol, Jaboticabal, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 07/51581-2
dc.description.sponsorshipIdFAPESP: 06/60696-5
dc.format.extent407-418
dc.identifierhttp://dx.doi.org/10.1007/s00424-011-0990-x
dc.identifier.citationPflugers Archiv-european Journal of Physiology. New York: Springer, v. 462, n. 3, p. 407-418, 2011.
dc.identifier.doi10.1007/s00424-011-0990-x
dc.identifier.issn0031-6768
dc.identifier.urihttp://hdl.handle.net/11449/2756
dc.identifier.wosWOS:000293954700004
dc.language.isoeng
dc.publisherSpringer
dc.relation.ispartofPflugers Archiv: European Journal of Physiology
dc.relation.ispartofjcr2.765
dc.relation.ispartofsjr1,479
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectCentral chemoreceptionen
dc.subjectPeripheral chemoreceptionen
dc.subjectHypercapniaen
dc.subjectPulmonary ventilationen
dc.subject5-HT neuronsen
dc.subjectMedullary rapheen
dc.titleSerotonergic neurons in the nucleus raphe obscurus contribute to interaction between central and peripheral ventilatory responses to hypercapniaen
dc.typeArtigo
dcterms.licensehttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dcterms.rightsHolderSpringer
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Agrárias e Veterinárias, Jaboticabalpt

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