Validation of an immunohistochemistry assay to detect turkey coronavirus: A rapid and simple screening tool for limited resource settings

Página do item simplificado
dc.contributor.authorCardoso, T. C. [UNESP]
dc.contributor.authorCastanheira, T. L. L. [UNESP]
dc.contributor.authorTeixeira, M. C. B. [UNESP]
dc.contributor.authorRosa, A. C. G. [UNESP]
dc.contributor.authorHirata, K. Y. [UNESP]
dc.contributor.authorAstolphi, R. D. [UNESP]
dc.contributor.authorLuvizotto, Maria Cecília Rui [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T15:31:10Z
dc.date.available2014-05-20T15:31:10Z
dc.date.issued2008-07-01
dc.description.abstractThe objective of the present study was to develop and apply the direct immunohistochemistry (D-IHC) assay to search for turkey coronavirus (TCoV) antigens in formalin-fixed embedded-paraffin tissues by the use of biotin-labeled polyclonal antibody. Twenty-eight-day-old embryonated turkey eggs (n = 50) were inoculated with TCoV-purified virus, and 3 d after inoculation, sections from ileum, ileum-cecal junction, and ceca were harvested, fixed in neutral formalin, and embedded in paraffin blocks and used as positive control. In addition, a total of 100 field samples from ileum, ileum-cecal junction, and ceca, collected from 30 to 45-d-old turkeys poults experiencing an outbreak of acute enteritis, were used to search for TCoV by the same D-IHC. All results were compared with those obtained by conventional RT-PCR and indirect fluorescent antibody assay (IFA) for all tested samples. Turkey coronavirus was detected in experimentally infected embryo tissues and also in field samples in 100% of ileum-cecal junction and ceca by the 3 detection procedures. With IFA as a reference assay, sensitivity and specificity of D-IHC were 98 and 58%, whereas sensitivity and specificity of reverse transcription-PCR were 96 and 66%, calculated from the total of tested samples from experimental infection. Each of the examined procedures was highly specific (D-IHC, 93%; RT-PCR, 90%), sensitive (D-IHC, 85%; RT-PCR, 86%), and agreement of both D-IHC and RT-PCR was 99 and 100%, respectively, compared with IFA results obtained from all the field samples. These findings demonstrated the utility of D-IHC for direct detection of TCoV from field samples and considering the sensitivity and specificity found here, can be used as an alternative technique.en
dc.description.affiliationSão Paulo State Univ, Virol Lab, Dept Apoio Prod & Saúde Anim, BR-16050680 São Paulo, Brazil
dc.description.affiliationUniv Estadual Paulista, Dept Clin Cirurgia & Reprod Anim, Lab Patol, BR-16050680 São Paulo, Brazil
dc.description.affiliationUnespSão Paulo State Univ, Virol Lab, Dept Apoio Prod & Saúde Anim, BR-16050680 São Paulo, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Dept Clin Cirurgia & Reprod Anim, Lab Patol, BR-16050680 São Paulo, Brazil
dc.format.extent1347-1352
dc.identifierhttp://dx.doi.org/10.3382/ps.2008-00042
dc.identifier.citationPoultry Science. Savoy: Poultry Science Assoc Inc, v. 87, n. 7, p. 1347-1352, 2008.
dc.identifier.doi10.3382/ps.2008-00042
dc.identifier.issn0032-5791
dc.identifier.lattes4584674909952477
dc.identifier.urihttp://hdl.handle.net/11449/40381
dc.identifier.wosWOS:000257388500014
dc.language.isoeng
dc.publisherPoultry Science Assoc Inc
dc.relation.ispartofPoultry Science
dc.relation.ispartofjcr2.216
dc.relation.ispartofsjr1,112
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectturkey coronavirusen
dc.subjectpoult enteritis and mortality syndromeen
dc.subjectimmunohistochemistryen
dc.titleValidation of an immunohistochemistry assay to detect turkey coronavirus: A rapid and simple screening tool for limited resource settingsen
dc.typeArtigo
dcterms.licensehttp://japr.fass.org/site/misc/Rights_Permissions.xhtml
dcterms.rightsHolderPoultry Science Assoc Inc
unesp.author.lattes4584674909952477
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina Veterinária, Araçatubapt

Arquivos

Licença do Pacote
Agora exibindo 1 - 2 de 2
Nenhuma Miniatura disponível
Nome:
license.txt
Tamanho:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descrição:
Nenhuma Miniatura disponível
Nome:
license.txt
Tamanho:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descrição:

Coleções

Artigos - Clínica, Cirurgia e Reprodução Animal - FMVA