Effects of intrapulpal temperature change induced by visible light units on the metabolism of odontoblast-like cells

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dc.contributor.authorChaves de Souza, Pedro Paulo
dc.contributor.authorHebling, Josimeri [UNESP]
dc.contributor.authorScalon, Mariana Grecco
dc.contributor.authorFabio Aranha, Andreza Maria [UNESP]
dc.contributor.authorde Souza Costa, Carlos Alberto [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2013-09-30T18:32:20Z
dc.date.accessioned2014-05-20T13:44:50Z
dc.date.available2013-09-30T18:32:20Z
dc.date.available2014-05-20T13:44:50Z
dc.date.issued2009-06-01
dc.description.abstractPurpose: To investigate the effects of intrapulpal temperature changes induced by a quartz tungsten halogen (QTH) and a light emitting diode (LED) curing units on the metabolism of odontoblast-like cells. Methods: Thirty-six 0.5 mm-thick dentin discs obtained from sound human teeth were randomly assigned into three groups: QTH, LED and no light (control). After placement of the dentin discs in pulp chamber devices, a thermistor was attached to the pulpal surface of each disc and the light sources were applied on the occlusal surface. After registering the temperature change, odontoblast-like cells MDPC-23 were seeded on the pulpal side of the discs and the curing lights were again applied. Cell metabolism was evaluated by the MTT assay and cell morphology was assessed by SEM. Results: In groups QTH and LED the intrapulpal temperature increased by 6.4 degrees C and 3.4 degrees C, respectively. The difference between both groups was statistically significant (Mann-Whitney; P< 0.05). QTH and LED reduced the cell metabolism by 36.4% and 33.4%, respectively. Regarding the cell metabolism, no statistically significant difference was observed between both groups (Mann-Whitney; P> 0.05). However, when compared to the control, only QTH significantly reduced the cell metabolism (Mann-Whitney; P< 0.05). It was concluded that the irradiance of 0.5 mm-thick human dentin discs with a QTH in comparison to a LED curing unit promoted a higher temperature rise, which propagates through the dentin negatively affecting the metabolism of the underlying cultured pulp cells. (Am J Dent 2009;22:151-156).en
dc.description.affiliationUniv São Paulo, UNESP, Sch Dent, Dept Physiol & Pathol, BR-14801903 Araraquara, SP, Brazil
dc.description.affiliationUniv São Paulo, UNESP, Dept Orthodont & Pediat Dent, BR-14801903 Araraquara, SP, Brazil
dc.description.affiliationUniv São Paulo, Fac Med Ribeirao Preto, USP, Dept Biochem & Immunol, Ribeirao Preto, SP, Brazil
dc.description.affiliationUnespUniv São Paulo, UNESP, Sch Dent, Dept Physiol & Pathol, BR-14801903 Araraquara, SP, Brazil
dc.description.affiliationUnespUniv São Paulo, UNESP, Dept Orthodont & Pediat Dent, BR-14801903 Araraquara, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipIdFAPESP: 07/50646-3
dc.description.sponsorshipIdFAPESP: 06/539063
dc.description.sponsorshipIdCNPq: 476137/2006-3
dc.description.sponsorshipIdCNPq: 301029/2007-5
dc.format.extent151-156
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/19650595
dc.identifier.citationAmerican Journal of Dentistry. Weston: Mosher & Linder, Inc, v. 22, n. 3, p. 151-156, 2009.
dc.identifier.issn0894-8275
dc.identifier.lattes4517484241515548
dc.identifier.urihttp://hdl.handle.net/11449/15727
dc.identifier.wosWOS:000268344000005
dc.language.isoeng
dc.publisherMosher & Linder, Inc
dc.relation.ispartofAmerican Journal of Dentistry
dc.relation.ispartofjcr0.760
dc.relation.ispartofsjr0,509
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.titleEffects of intrapulpal temperature change induced by visible light units on the metabolism of odontoblast-like cellsen
dc.typeArtigo
dcterms.rightsHolderMosher & Linder, Inc
unesp.author.lattes4517484241515548[5]
unesp.author.orcid0000-0001-7266-6061[1]
unesp.author.orcid0000-0002-7455-6867[5]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Odontologia, Araraquarapt

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