Origin and distribution of AT-rich repetitive DNA families in Triatoma infestans (Heteroptera)

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dc.contributor.authorBardella, Vanessa Bellini [UNESP]
dc.contributor.authorRosa, João Aristeu da [UNESP]
dc.contributor.authorLaforga Vanzela, Andre Luis
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Estadual de Londrina (UEL)
dc.date.accessioned2014-12-03T13:11:07Z
dc.date.available2014-12-03T13:11:07Z
dc.date.issued2014-04-01
dc.description.abstractTriatoma infestans, one of the most important vectors of Trypanosoma cruzi, is very interesting model, because it shows large interpopulation variation in the amount and distribution of heterochromatin. This polymorphism involved the three large pairs up to almost all autosomal pairs, including the sex chromosomes. To understand the dynamics of heterochromatin variation in T. infestans, we isolated the AT-rich satDNA portion of this insect using reassociation kinetics (C(0)t), followed by cloning, sequencing and FISH. After chromosome localization, immunolabeling with anti-5-methylcytosine, anti-H4K5ac and anti-H3K9me2 antibodies was performed to determine the functional characteristics of heterochromatin. The results allowed us to reorganize the karyotype of T. infestans in accordance with the distribution of the families of repetitive DNA using seven different markers. We found that two arrays with lengths of 79 and 33 bp have a strong relationship with transposable element sequences, suggesting that these two families of satDNA probably originated from Polintons. The results also allowed us to identify at least four chromosome rearrangements involved in the amplification/dispersion of AT-rich satDNA of T. infestans. These data should be very useful in new studies including those examining the cytogenomic and population aspects of this very important species of insect. (C) 2014 Elsevier B.V. All rights reserved.en
dc.description.affiliationIBILCE UNESP, Dept Biol, Inst Biociencias Letras & Ciencias Exatas, BR-15054000 Sao Jose Do Rio Preto, SP, Brazil
dc.description.affiliationFCFAR UNESP, Fac Ciencias Famaceut Araraquara, Dept Ciencias Biol, BR-14801902 Araraquara, SP, Brazil
dc.description.affiliationUniv Estadual Londrina, CCB, Dept Biol Geral, BR-86051990 Londrina, Parana, Brazil
dc.description.affiliationUnespIBILCE UNESP, Dept Biol, Inst Biociencias Letras & Ciencias Exatas, BR-15054000 Sao Jose Do Rio Preto, SP, Brazil
dc.description.affiliationUnespFCFAR UNESP, Fac Ciencias Famaceut Araraquara, Dept Ciencias Biol, BR-14801902 Araraquara, SP, Brazil
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipFundacao Araucaria
dc.format.extent106-114
dc.identifierhttp://dx.doi.org/10.1016/j.meegid.2014.01.035
dc.identifier.citationInfection Genetics And Evolution. Amsterdam: Elsevier Science Bv, v. 23, p. 106-114, 2014.
dc.identifier.doi10.1016/j.meegid.2014.01.035
dc.identifier.issn1567-1348
dc.identifier.urihttp://hdl.handle.net/11449/112875
dc.identifier.wosWOS:000335107100014
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofInfection, Genetics and Evolution
dc.relation.ispartofjcr2.545
dc.relation.ispartofsjr1,278
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectHeterochromatinen
dc.subjectHolocentric chromosomesen
dc.subjectImmunolabelingen
dc.subjectMolecular cytogeneticen
dc.subjectSatDNAen
dc.subjectReduviidaeen
dc.titleOrigin and distribution of AT-rich repetitive DNA families in Triatoma infestans (Heteroptera)en
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Farmacêuticas, Araraquarapt
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências Letras e Ciências Exatas, São José do Rio Pretopt

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