Comparison of uroplakin expression during urothelial carcinogenesis induced by N-butyl-N-(4-hydroxybutyl)nitrosamine in rats and mice

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dc.contributor.authorOgawa, Kumiko
dc.contributor.authorSt. John, Margaret
dc.contributor.authorDe Oliveira, Maria Luiza [UNESP]
dc.contributor.authorArnold, Lora
dc.contributor.authorShirai, Tomoyuki
dc.contributor.authorSun, Tung-Tien
dc.contributor.authorCohen, Samuel Monroe [UNESP]
dc.contributor.institutionUniversidade de Nebraska-Lincoln (UNL)
dc.contributor.institutionNagoya University
dc.contributor.institutionNew York University (NYU)
dc.contributor.institutionNebraska Medical Center
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-27T11:19:51Z
dc.date.available2014-05-27T11:19:51Z
dc.date.issued1999-12-08
dc.description.abstractThe expression of uroplakins, the tissue-specific and differentiation- dependent membrane proteins of the urothelium, was analyzed immunohistochemically in N butyl-N-(4-hydroxybutyl)nitrosamine (BBN)-treated rats and mice during bladder carcinogenesis. Male Fischer 344 rats were treated with 0.05% BBN in the drinking water for 10 wk and were cuthanatized at week 20 of the experiment. BBN was administered to male B6D2F1 mice; it was either provided at a rate of 0.05% in the drinking water (for 26 wk) or 5 mg BBN was administered by intragastric gavage twice weekly for 10 wk, followed by 20 wk without treatment. In rats, BBN-induced, noninvasive, low grade, papillary, transitional cell carcinoma (TCC) showed decreased uroplakin-staining of cells lining the lumen but showed increased expression in some nonluminal cells. In mice, nonpapillary, high-grade dysplasia, carcinoma in situ, and invasive carcinoma were induced. There was a marked decrease in the number of uroplakin-positive cells lining the lumen and in nonluminal cells. This occurred in normal-appearing urothelium in BBN-treated mice and in dysplasic urothelium, in carcinoma in situ, and in invasive TCC. The percentage of uroplakin-positive nonluminal cells was higher in control mice than in rats, but it was lower in the mouse than in the rat after BBN treatment. Uroplakin expression was disorderly and focal in BBN-treated urothelium in both species. These results indicate that BBN treatment changed the expression of uroplakins during bladder carcinogenesis, with differences in rats and mice being related to degree of tumor differentiation.en
dc.description.affiliationDept. of Pathology and Microbiology Eppley Inst. for Research in Cancer Univ. of Nebraska Medical Center, Omaha, NE 68198-3135
dc.description.affiliationFirst Department of Pathology Nagoya City Univ. Medical School, Nagoya 454
dc.description.affiliationDepartment of Pharmacology Kaplan Comprehensive Cancer Center New York Univ. School of Medicine, New York, NY 10016
dc.description.affiliationDept. of Pathology and Microbiology Univ. of Nebraska Medical Center 983135 Nebraska Medical Center, Omaha, NE 68198-3135
dc.description.affiliationDepartamento de Patologia Faculdade de Medicina Unesp, Botucatu-SP, CEP, 18618-000
dc.description.affiliationUnespDepartamento de Patologia Faculdade de Medicina Unesp, Botucatu-SP, CEP, 18618-000
dc.format.extent645-651
dc.identifierhttp://dx.doi.org/10.1177/019262339902700606
dc.identifier.citationToxicologic Pathology, v. 27, n. 6, p. 645-651, 1999.
dc.identifier.doi10.1177/019262339902700606
dc.identifier.file2-s2.0-0032722952.pdf
dc.identifier.issn0192-6233
dc.identifier.scopus2-s2.0-0032722952
dc.identifier.urihttp://hdl.handle.net/11449/66035
dc.language.isoeng
dc.relation.ispartofToxicologic Pathology
dc.relation.ispartofjcr1.966
dc.relation.ispartofsjr0,807
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectBladder cancer
dc.subjectCell membranes
dc.subjectDifferentiation
dc.subjectUroplakins
dc.subjectdrinking water
dc.subjectmembrane protein
dc.subjectn butyl n (4 hydroxybutyl)nitrosamine
dc.subjectanimal experiment
dc.subjectanimal model
dc.subjectbladder cancer
dc.subjectbladder carcinogenesis
dc.subjectcancer grading
dc.subjectcarcinoma in situ
dc.subjectdysplasia
dc.subjectimmunohistochemistry
dc.subjectmale
dc.subjectmouse
dc.subjectnonhuman
dc.subjectpriority journal
dc.subjectprotein expression
dc.subjectrat
dc.subjectreview
dc.subjectstomach lavage
dc.subjecttransitional cell carcinoma
dc.subjecttumor differentiation
dc.subjectAnimals
dc.subjectButylhydroxybutylnitrosamine
dc.subjectCarcinogenicity Tests
dc.subjectCarcinogens
dc.subjectCarcinoma
dc.subjectCell Count
dc.subjectImmunohistochemistry
dc.subjectMale
dc.subjectMembrane Glycoproteins
dc.subjectMembrane Proteins
dc.subjectMice
dc.subjectRats
dc.subjectRats, Inbred F344
dc.subjectUrinary Bladder Neoplasms
dc.subjectUrothelium
dc.titleComparison of uroplakin expression during urothelial carcinogenesis induced by N-butyl-N-(4-hydroxybutyl)nitrosamine in rats and miceen
dc.typeArtigo
dcterms.licensehttp://www.sagepub.com/authors/journal/permissions.sp#7
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina, Botucatupt

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