The effect of dimethyl sulfoxide (DMSO) on dentin bonding and nanoleakage of etch-and-rinse adhesives

Página do item simplificado
dc.contributor.authorTjäderhane, Leo
dc.contributor.authorMehtälä, Pekka
dc.contributor.authorScaffa, Polliana
dc.contributor.authorVidal, Cristina
dc.contributor.authorPääkkönen, Virve
dc.contributor.authorBreschi, Lorenzo
dc.contributor.authorHebling, Josimeri [UNESP]
dc.contributor.authorTay, Franklin R.
dc.contributor.authorNascimento, Fabio D.
dc.contributor.authorPashley, David H.
dc.contributor.authorCarrilho, Marcela R.
dc.contributor.institutionOulu University Hospital
dc.contributor.institutionUniversity of Turku
dc.contributor.institutionNordic Institute of Dental Materials (NIOM)
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.contributor.institutionUniversity of Trieste
dc.contributor.institutionC/o IOR
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionMedical College of Georgia
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversity of Western Ontario
dc.date.accessioned2014-05-27T11:30:46Z
dc.date.available2014-05-27T11:30:46Z
dc.date.issued2013-10-01
dc.description.abstractObjective The objective was to examine the effect of a solvent dimethyl sulfoxide (DMSO) on resin-dentin bond durability, as well as potential functional mechanisms behind the effect. Methods Microtensile bond strength (μTBS) was evaluated in extracted human teeth in two separate experiments. Dentin specimens were acid-etched and assigned to pre-treatment with 0.5 mM (0.004%) DMSO as additional primer for 30 s and to controls with water pre-treatment. Two-step etch-and-rinse adhesive (Scotchbond 1XT, 3M ESPE) was applied and resin composite build-ups were created. Specimens were immediately tested for μTBS or stored in artificial saliva for 6 and 12 months prior to testing. Additional immediate and 6-month specimens were examined for interfacial nanoleakage analysis under SEM. Matrix metalloproteinase (MMP) inhibition by DMSO was examined with gelatin zymography. Demineralized dentin disks were incubated in 100% DMSO to observe the optical clearing effect. Results The use of 0.5 mM DMSO had no effect on immediate bond strength or nanoleakage. In controls, μTBS decreased significantly after storage, but increased significantly in DMSO-treated group. The control group had significantly lower μTBS than DMSO-group after 6 and 12 months. DMSO also eliminated the increase in nanoleakage seen in controls. 5% and higher DMSO concentrations significantly inhibited the gelatinases. DMSO induced optical clearing effect demonstrating collagen dissociation. Significance DMSO as a solvent may be useful in improving the preservation of long-term dentin-adhesive bond strength. The effect may relate to dentinal enzyme inhibition or improved wetting of collagen by adhesives. The collagen dissociation required much higher DMSO concentrations than the 0.5 mM DMSO used for bonding. © 2013 Academy of Dental Materials.en
dc.description.affiliationInstitute of Dentistry University of Oulu Oulu University Hospital, PO Box 5281, 90014 Oulu
dc.description.affiliationInstitute of Dentistry University of Turku, Turku
dc.description.affiliationNordic Institute of Dental Materials (NIOM), Oslo
dc.description.affiliationEstate University of Campinas Piracicaba Dental School (FOP/UNICAMP), São Paulo
dc.description.affiliationDepartment of Biomedicine Unit of Dental Sciences and Biomaterials University of Trieste, Trieste
dc.description.affiliationIGM-CNR Unit of Bologna C/o IOR, Bologna
dc.description.affiliationDepartment of Orthodontics and Pediatric Dentistry Araraquara School of Dentistry São Paulo State University UNESP, Araraquara, SP
dc.description.affiliationDepartment of Oral Biology and Maxillofacial Pathology School of Dentistry Medical College of Georgia, Augusta, GA
dc.description.affiliationBandeirante University of São Paulo (UNIBAN), São Paulo
dc.description.affiliationSchulich Dentistry University of Western Ontario, London, ON
dc.description.affiliationUnespDepartment of Orthodontics and Pediatric Dentistry Araraquara School of Dentistry São Paulo State University UNESP, Araraquara, SP
dc.format.extent1055-1062
dc.identifierhttp://dx.doi.org/10.1016/j.dental.2013.07.014
dc.identifier.citationDental Materials, v. 29, n. 10, p. 1055-1062, 2013.
dc.identifier.doi10.1016/j.dental.2013.07.014
dc.identifier.issn0109-5641
dc.identifier.scopus2-s2.0-84884137687
dc.identifier.urihttp://hdl.handle.net/11449/76702
dc.identifier.wosWOS:000324237700010
dc.language.isoeng
dc.relation.ispartofDental Materials
dc.relation.ispartofjcr4.039
dc.relation.ispartofsjr2,106
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectDentin bonding
dc.subjectDMSO
dc.subjectDurability
dc.subjectHuman
dc.subjectLong-term
dc.subjectMatrix metalloproteinase
dc.subjectNanoleakage
dc.subjectMatrix metalloproteinases
dc.subjectNanoleakages
dc.subjectAdhesives
dc.subjectBond (masonry)
dc.subjectBond strength (materials)
dc.subjectCollagen
dc.subjectDimethyl sulfoxide
dc.subjectDissociation
dc.subjectEnzyme inhibition
dc.subjectResins
dc.subjectWater treatment
dc.subjectOrganic solvents
dc.titleThe effect of dimethyl sulfoxide (DMSO) on dentin bonding and nanoleakage of etch-and-rinse adhesivesen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
unesp.author.orcid0000-0002-3887-4091[3]
unesp.author.orcid0000-0001-9770-8598[5]
unesp.author.orcid0000-0002-5018-478X[1]
unesp.author.orcid0000-0001-7621-226X[6]
unesp.author.orcid0000-0003-4345-8487[4]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Odontologia, Araraquarapt

Arquivos

Coleções

Artigos - Clínica Infantil - FOAR