Control of microorganisms of oral health interest with Arctium lappa L. (burdock) extract non-cytotoxic to cell culture of macrophages (RAW 264.7)

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dc.contributor.authorOliveira, Jonatas Rafael de [UNESP]
dc.contributor.authorAguiar Almeida, Rosilene Batista de [UNESP]
dc.contributor.authorFigueiredo Vilela, Polyana das Gracas [UNESP]
dc.contributor.authorOliveira, Felipe Eduardo de [UNESP]
dc.contributor.authorRocha, Rosilene Fernandes da [UNESP]
dc.contributor.authorCardoso Jorge, Antonio Olavo [UNESP]
dc.contributor.authorOliveira, Luciane Dias de [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-12-03T13:10:55Z
dc.date.available2014-12-03T13:10:55Z
dc.date.issued2014-08-01
dc.description.abstractObjectives: To evaluate the antimicrobial activity of Arctium lappa L. extract on Staphylococcus aureus, S. epidermidis, Streptococcus mutans, Candida albicans, C. tropicalis and C. glabrata. In addition, the cytotoxicity of this extract was analyzed on macrophages (RAW 264.7).Design: By broth microdilution method, different concentrations of the extract (250-0.4 mg/mL) were used in order to determine the minimum microbicidal concentration (MMC) in planktonic cultures and the most effective concentration was used on biofilms on discs made of acrylic resin. The cytotoxicity A. lappa L. extract MMC was evaluated on RAW 264.7 by MTT assay and the quantification of IL-1 beta and TNF-alpha by ELISA.Results: The most effective concentration was 250 mg/mL and also promoted significant reduction (log(10)) in the biofilms of S. aureus (0.438 +/- 0.269), S. epiderrnidis (0.377 +/- 0.298), S. mutans (0.244 +/- 0.161) and C. albicans (0.746 +/- 0.209). Cell viability was similar to 100%. The production of IL-beta was similar to the control group (p > 0.05) and there was inhibition of TNF-alpha (p < 0.01).Conclusions: A. lappa L. extract was microbicidal for all the evaluated strains in planktonic cultures, microbiostatic for biofilms and not cytotoxic to the macrophages. (C) 2014 Elsevier Ltd. All rights reserved.en
dc.description.affiliationUniv Estadual Paulista, UNESP, Dept Biosci & Oral Diag, Lab Microbiol & Immunol,Inst Sci & Technol, BR-12245000 Sao Jose Dos Campos, SP, Brazil
dc.description.affiliationUniv Estadual Paulista, UNESP, Dept Biosci & Oral Diag, Lab Biochem & Pharmacol,Inst Sci & Technol, BR-12245000 Sao Jose Dos Campos, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, UNESP, Dept Biosci & Oral Diag, Lab Microbiol & Immunol,Inst Sci & Technol, BR-12245000 Sao Jose Dos Campos, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, UNESP, Dept Biosci & Oral Diag, Lab Biochem & Pharmacol,Inst Sci & Technol, BR-12245000 Sao Jose Dos Campos, SP, Brazil
dc.format.extent808-814
dc.identifierhttp://dx.doi.org/10.1016/j.archoralbio.2014.05.013
dc.identifier.citationArchives Of Oral Biology. Oxford: Pergamon-elsevier Science Ltd, v. 59, n. 8, p. 808-814, 2014.
dc.identifier.doi10.1016/j.archoralbio.2014.05.013
dc.identifier.issn0003-9969
dc.identifier.lattes0190365234516027
dc.identifier.lattes0053567153623569
dc.identifier.lattes4716676611330311
dc.identifier.urihttp://hdl.handle.net/11449/112648
dc.identifier.wosWOS:000338822300006
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofArchives of Oral Biology
dc.relation.ispartofjcr2.050
dc.relation.ispartofsjr0,752
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectArctium lappaen
dc.subjectBiofilmsen
dc.subjectStaphylococcusen
dc.subjectStreptococcus mutansen
dc.subjectCandidaen
dc.subjectRAW 264.7en
dc.titleControl of microorganisms of oral health interest with Arctium lappa L. (burdock) extract non-cytotoxic to cell culture of macrophages (RAW 264.7)en
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.
unesp.author.lattes0190365234516027
unesp.author.lattes0053567153623569[6]
unesp.author.lattes4716676611330311
unesp.author.orcid0000-0002-1747-6158[6]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Ciência e Tecnologia, São José dos Campospt
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt

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