Fibra de carbono: caracterização e avaliação da resposta inflamatória e toxicidade em ratos
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Data
2022-08-01
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Universidade Estadual Paulista (Unesp)
Resumo
Este estudo teve como objetivo avaliar a Fibra de Carbono obtida a partir da fibra PAN têxtil e de fibra de algodão, nas suas diferentes formas de apresentação: Feltro Fibra de Carbono Não Ativado (FFCNA), Feltro Fibra de Carbono Ativado (FFCA), Feltro de Fibra de Carbono Ativado com Prata (FFCAAg) e Tecido Fibra de Carbono Ativado (TFCA), com vistas à obtenção de scaffolds como potencial material com propriedades relativas à enxerto ósseo sintético. Foram realizados testes de caracterização: molhabilidade de superfície, ensaio de tração, ensaio de intumescimento e testes in vivo: avaliação da resposta inflamatória pela implantação dos materiais no tecido subcutâneo de quatorze ratos Wistar, avaliação das fibras colágenas pela coloração Picrosirius Red e avaliação da toxicidade nos seguintes órgãos: coração, baço, fígado e rim. No teste de molhabilidade, FFCNA e TFCA se mostraram hidrofóbicos (ɵ124º e 114º), FFCA e FFCAAg hidrofílicos. Para tensão máxima FFCA se apresentou mais resistente (2.983 ± 1.059). No ensaio de intumescimento os grupos FFCAAg e FFCA se mostraram com maior porcentagem de absorção para a solução PBS e água destilada. Os animais não apresentaram sinais clínicos de intoxicação. Os órgãos não apresentaram sinais de toxicidade sistêmica aguda. As regiões dos implantes apresentaram infiltrado inflamatório de leve a moderado em 7 e 21 dias. Apenas o grupo TFCA não apresentou maturação de fibras colágenas tipo I em 21 dias. Por meio das análises relizadas constatou-se que o TFCA se apresentou hidrofóbico, pouco resistente a tração e baixo potencial para maturação de fibras colágenas. Portanto o TFCA mostra-se com pouco potencial para ser indicado como possível scaffold para engenharia tecidual óssea. Conclui-se que FFCNA, FFCA e FFCAAg, possuem potencial para serem consideradas scaffolds, devido as seguintes características que foram apresentadas: boa taxa de absorção, hidrofilicidade e atóxicas.
This study aimed to evaluate the Carbon Fiber obtained from PAN textile fiber and cotton fiber, in its different forms of presentation: Non-Activated Carbon Fiber Felt (FFCNA), Activated Carbon Fiber Felt (FFCA), Activated Carbon Fiber with Silver (FFCAAg) and Activated Carbon Fiber Tissue (TFCA), with a view to obtaining scaffolds as a potential material with properties related to synthetic bone graft. Characterization tests were performed: surface wettability, traction test, swelling test and in vivo tests: evaluation of the inflammatory response by implanting the materials in the subcutaneous tissue of fourteen Wistar rats, evaluation of collagen fibers by Picrosirius Red staining and evaluation of toxicity in the following organs: heart, spleen, liver and kidney. In the wettability test, FFCNA and TFCA were hydrophobic (ɵ124º and 114º), FFCA and FFCAAg were hydrophilic. For maximum stress FFCA was more resistant (2.983 ± 1.059). In the swelling test, the FFCAag and FFCA groups showed the highest percentage of absorption for the PBS solution and distilled water. The animals did not show clinical signs of intoxication. The organs showed no signs of acute systemic toxicity. The implant regions showed mild to moderate inflammatory infiltrate at 7 and 21 days. Only the TFCA group did not show maturation of type I collagen fibers in 21 days. Through the analyzes carried out, it was found that the TFCA was hydrophobic, with little resistance to traction and low potential for collagen fiber maturation. Therefore, TFCA shows little potential to be indicated as a possible scaffold for bone tissue engineering. It is concluded that FFCNA, FFCA and FFCAAg have the potential to be considered scaffolds, due to the following characteristics that were presented: good absorption rate, hydrophilicity and non-toxic.
This study aimed to evaluate the Carbon Fiber obtained from PAN textile fiber and cotton fiber, in its different forms of presentation: Non-Activated Carbon Fiber Felt (FFCNA), Activated Carbon Fiber Felt (FFCA), Activated Carbon Fiber with Silver (FFCAAg) and Activated Carbon Fiber Tissue (TFCA), with a view to obtaining scaffolds as a potential material with properties related to synthetic bone graft. Characterization tests were performed: surface wettability, traction test, swelling test and in vivo tests: evaluation of the inflammatory response by implanting the materials in the subcutaneous tissue of fourteen Wistar rats, evaluation of collagen fibers by Picrosirius Red staining and evaluation of toxicity in the following organs: heart, spleen, liver and kidney. In the wettability test, FFCNA and TFCA were hydrophobic (ɵ124º and 114º), FFCA and FFCAAg were hydrophilic. For maximum stress FFCA was more resistant (2.983 ± 1.059). In the swelling test, the FFCAag and FFCA groups showed the highest percentage of absorption for the PBS solution and distilled water. The animals did not show clinical signs of intoxication. The organs showed no signs of acute systemic toxicity. The implant regions showed mild to moderate inflammatory infiltrate at 7 and 21 days. Only the TFCA group did not show maturation of type I collagen fibers in 21 days. Through the analyzes carried out, it was found that the TFCA was hydrophobic, with little resistance to traction and low potential for collagen fiber maturation. Therefore, TFCA shows little potential to be indicated as a possible scaffold for bone tissue engineering. It is concluded that FFCNA, FFCA and FFCAAg have the potential to be considered scaffolds, due to the following characteristics that were presented: good absorption rate, hydrophilicity and non-toxic.
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Palavras-chave
Fibra de carbono, Testes mecânicos, Materiais biocompatíveis, Engenharia tecidual, Carbon fiber, Mechanical tests, Biocompatible materials, Tissue engineering.