Propriedades físico-químicas e antibiofilme de materiais endodônticos biocerâmicos obturadores e reparadores e associações com cetramida
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2022-03-29
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Universidade Estadual Paulista (Unesp)
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Objetivo: Materiais biocerâmicos devem apresentar propriedades físico-químicas e antibiofilme adequadas. Foram avaliados (Publicação 1): cimentos biocerâmicos reparadores: NeoPutty (Nusmile, EUA) e MTA Repair HP (Angelus, Brasil) (Publicação 2): e os cimentos endodônticos biocerâmicos Neo-MTA 2 (Nusmile, EUA) e BioRoot RCS (Septodont, França) associados ou não a cetramida (CTR) 1%. Metodologia: Tempo de presa (TP) foi avaliado segundo norma ISO-6876 empregando agulha Gilmore. Tubos de polietileno preenchidos com os cimentos foram utilizados para avaliação do pH nos períodos de 1, 3, 7, 14, 21 e 28 dias da imersão em água deionizada. Tubos de dentina bovina foram imersos em água destilada por 24 h a 37C, preenchidos com os cimentos, e depois foi realizado escaneamento em Microtomografia computadorizada (Micro-CT. SkyScan 1176. Bruker, Bélgica) a 9 µm. Após imersão dos cimentos reparadores em PBS por 7 dias e dos cimentos obturadoes por 7 e 28 dias, novo escaneamento foi realizado para avaliação da alteração volumétrica e porosidade. A reconstrução (NRecon - V1.6.4,7; SkyScan, Bélgica), sobreposição (Data Viewer - V1.5.2.4; SkyScan, Bélgica) e análise (CTAn - V1.15.4.0; SkyScan, Bélgica) das imagens obtidas com os escaneamentos foi realizada. Modelos 3D das cavidades foram obtidos por meio do programa CTVol (V2.3.1.0). A atividade antibiofilme, foi avaliada pelo teste de contato direto modificado com biofilme (TCDM). Os dados obtidos foram submetidos aos testes estatísticos adequados para cada experimento com nível de significância de 5%. Publicação 1 – Resultados: NeoPutty apresentou maior TP que MTA Repair HP (p<0,05). MTA Repair HP apresentou maiores valores de pH (p<0,05). Em todos os períodos, os dois cimentos apresentaram pH alcalino. NeoPutty demonstrou maior solubilidade (p<0.05) que MTA-HP. Porém, ambos materiais apresentaram menos que 3% de solubilidade. A alteração volumétrica e porosidade apresentaram valores menores que 1%, sem diferença significativa entre materiais (p>0.05). Ambos os cimentos apresentaram ação antibiofilme em comparação ao grupo controle, sem diferença estatística entre si (p>0.05). Conclusões: NeoPutty e MTA Repair HP apresentam pH alcalino, baixa solubilidade, baixa porosidade e estabilidade dimensional, além de ação antibiofilme, sugerindo a aplicação clínica. Publicação 2 – Resultados: BR e BR+CTR apresentaram menor TP que NMTA2 e NMTA2+CTR (p < 0,05). Em todos os períodos, os cimentos avaliados e associações apresentaram pH alcalino. Não houve diferença significativa na alteração volumétrica entre a extremidade e a parte interna dos espécimes (p>0.05). Os grupos que apresentaram diferença estatística entre os períodos de 7 e 28 dias (p<0.05) são NEO MTA2 na análise da extremidade e NEO+CTR na análise da parte interna do espécime, sendo que após 28 dias os materiais apresentam diminuição do volume. As associações com CTR demonstraram maior atividade antibiofilme (p<0,5) quando comparados com os cimentos sem associação. Conclusões: Os materiais apresentaram adequados tempo de presa e pH, atividade antibiofilme e baixa alteração volumétrica. A associação com CTR não prejudicou as propriedades físico-químicas, além de aumentar a atividade antibiofilme de Neo-MTA 2 e BioRoot RCS.
Objective: Bioceramic materials must have physicochemical and antibiofilm properties. We evaluated (Publication 1): bioceramic repair materials: NeoPutty (Nusmile, USA) and MTA Repair HP (Angelus, Brazil) and the bioceramic endodontic cements (Publication 2): Neo-MTA 2 (Nusmile, USA) and BioRoot RCS (Septodont, France) associated or not with 1% cetrimide (CTR). Methodology: Setting time (ST) was evaluated according to ISO-6876 using Gilmore needle. Polyethylene tubes filled with cement were used to evaluate the pH in the periods of 1, 3, 7, 14, 21 and 28 days of immersion in deionized water. Bovine dentin tubes were immersed in distilled water for 24 h at 37°C, filled with cements, and then scanned in Computerized Microtomography (Micro-CT. SkyScan 1176. Bruker, Belgium) at 9 µm. After immersion of the repair cements in PBS for 7 days and the endodontics cements for 7 and 28 days, a new scan was performed to evaluate the volumetric change and porosity. The reconstruction (NRecon - V1.6.4.7; SkyScan, Belgium), overlay (Data Viewer - V1.5.2.4; SkyScan, Belgium) and analysis (CTAn - V1.15.4.0; SkyScan, Belgium) of the images obtained with scans were performed. 3D models of the cavities were obtained using the CTVol program (V2.3.1.0). Antibiofilm activity was evaluated by the modified direct contact test with biofilm. The data obtained were submitted to the appropriate statistical tests for each experiment with a significance level of 5%. Publication 1 – Results: NeoPutty presented higher ST than MTA Repair HP (p<0.05). MTA Repair HP showed higher pH values (p<0.05). In all periods, the two cements presented alkaline pH. NeoPutty demonstrated greater solubility (p<0.05) than MTA-HP. However, both materials showed less than 3% solubility. The volumetric change and porosity presented values lower than 1%, with no significant difference between materials (p>0.05). Both cements showed antibiofilm action compared to the control group, with no statistical difference between them (p>0.05). Conclusions: NeoPutty and MTA Repair HP have alkaline pH, low solubility, low porosity and dimensional stability, in addition to antibiofilm action, suggesting clinical application. Publication 2 – Results: BR and BR+CTR showed lower ST than NMTA2 and NMTA2+CTR (p < 0.05). In all periods, the evaluated cements and associations presented alkaline pH. There was no significant difference in volumetric change between the tip and the inside of the specimens (p>0.05). The groups that presented statistical difference between the periods of 7 and 28 days (p<0.05) are NEO MTA2 in the analysis of the extremity and NEO+CTR in the analysis of the internal part of the specimen, and after 28 days the materials show a decrease in volume. Associations with CTR showed greater antibiofilm activity (p<0.5) when compared with cements without association. Conclusions: The materials showed adequate setting time and pH, antibiofilm activity and low volumetric change. The association with CTR did not impair the physicochemical properties, in addition to increasing the antibiofilm activity of Neo-MTA 2 and BioRoot RCS.
Objective: Bioceramic materials must have physicochemical and antibiofilm properties. We evaluated (Publication 1): bioceramic repair materials: NeoPutty (Nusmile, USA) and MTA Repair HP (Angelus, Brazil) and the bioceramic endodontic cements (Publication 2): Neo-MTA 2 (Nusmile, USA) and BioRoot RCS (Septodont, France) associated or not with 1% cetrimide (CTR). Methodology: Setting time (ST) was evaluated according to ISO-6876 using Gilmore needle. Polyethylene tubes filled with cement were used to evaluate the pH in the periods of 1, 3, 7, 14, 21 and 28 days of immersion in deionized water. Bovine dentin tubes were immersed in distilled water for 24 h at 37°C, filled with cements, and then scanned in Computerized Microtomography (Micro-CT. SkyScan 1176. Bruker, Belgium) at 9 µm. After immersion of the repair cements in PBS for 7 days and the endodontics cements for 7 and 28 days, a new scan was performed to evaluate the volumetric change and porosity. The reconstruction (NRecon - V1.6.4.7; SkyScan, Belgium), overlay (Data Viewer - V1.5.2.4; SkyScan, Belgium) and analysis (CTAn - V1.15.4.0; SkyScan, Belgium) of the images obtained with scans were performed. 3D models of the cavities were obtained using the CTVol program (V2.3.1.0). Antibiofilm activity was evaluated by the modified direct contact test with biofilm. The data obtained were submitted to the appropriate statistical tests for each experiment with a significance level of 5%. Publication 1 – Results: NeoPutty presented higher ST than MTA Repair HP (p<0.05). MTA Repair HP showed higher pH values (p<0.05). In all periods, the two cements presented alkaline pH. NeoPutty demonstrated greater solubility (p<0.05) than MTA-HP. However, both materials showed less than 3% solubility. The volumetric change and porosity presented values lower than 1%, with no significant difference between materials (p>0.05). Both cements showed antibiofilm action compared to the control group, with no statistical difference between them (p>0.05). Conclusions: NeoPutty and MTA Repair HP have alkaline pH, low solubility, low porosity and dimensional stability, in addition to antibiofilm action, suggesting clinical application. Publication 2 – Results: BR and BR+CTR showed lower ST than NMTA2 and NMTA2+CTR (p < 0.05). In all periods, the evaluated cements and associations presented alkaline pH. There was no significant difference in volumetric change between the tip and the inside of the specimens (p>0.05). The groups that presented statistical difference between the periods of 7 and 28 days (p<0.05) are NEO MTA2 in the analysis of the extremity and NEO+CTR in the analysis of the internal part of the specimen, and after 28 days the materials show a decrease in volume. Associations with CTR showed greater antibiofilm activity (p<0.5) when compared with cements without association. Conclusions: The materials showed adequate setting time and pH, antibiofilm activity and low volumetric change. The association with CTR did not impair the physicochemical properties, in addition to increasing the antibiofilm activity of Neo-MTA 2 and BioRoot RCS.
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Materiais dentários, Propriedades físicas e químicas, Biofilmes, Microtomografia por raio-x, Endodontia, Dental materials, Physical and chemical properties, Biofilms, X-ray microtomography, Endodontics