Analysis of total glucosinolates and chromatographically purified benzylglucosinolate in organic and conventional vegetables

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dc.contributor.authorMiranda Rossetto, Maria Rosecler [UNESP]
dc.contributor.authorShiga, Tânia Mizuzo
dc.contributor.authorVianello, Fabio
dc.contributor.authorPereira Lima, Giuseppina Pace [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversity of Padova
dc.date.accessioned2014-05-27T11:27:28Z
dc.date.available2014-05-27T11:27:28Z
dc.date.issued2013-01-01
dc.description.abstractThe limited availability of foods that are free of pesticides has led Brazil to search for alternative production methods to meet the desires of consumers. Currently, organic cultivation represents a production system that complies with general expectations of producers and consumers. Organic cultivation is particularly interesting mainly because of its effect on plant secondary metabolite content, which may help plants to naturally combat pests; in humans, these substances can also contribute to the prevention of chronic diseases. We report on the extraction of glucosinolates (both as total glucosinolates and as benzylglucosinolate) with trifluoroacetic acid addition in a 70:30 MeOH:water (v/v). Total glucosinolates, determined by a thioglucosidase coupled assay, were measured in different Brassicaceae species and were similar to values reported in the literature. For broccoli, analyses were carried out separately on inflorescences, leaves and stalks; analyses were also conducted on thermally processed samples to simulate cooking. Furthermore, when the analysis was conducted on conventional and organic products, the highest concentrations of these substances were most often found in organically cultivated Brassicaceae. The benzylglucosinolate concentrations were evaluated on the same samples using HPLC. The concentration of benzylglucosinolate was significantly higher in organically cultivated vegetables, as well. © 2012 Elsevier Ltd.en
dc.description.affiliationDepartment of Chemistry and Biochemistry Biosciences Institute Universidade Estadual Paulista, UNESP, Rubião Junior CP 510, 18618-000 Botucatu, São Paulo
dc.description.affiliationDepartment of Food Science Faculty Pharmaceutical Science Universidade de São Paulo, USP, São Paulo
dc.description.affiliationDepartment of Comparative Biomedicine and Food Science University of Padova, Padova
dc.description.affiliationUnespDepartment of Chemistry and Biochemistry Biosciences Institute Universidade Estadual Paulista, UNESP, Rubião Junior CP 510, 18618-000 Botucatu, São Paulo
dc.format.extent247-252
dc.identifierhttp://dx.doi.org/10.1016/j.lwt.2012.05.022
dc.identifier.citationLWT - Food Science and Technology, v. 50, n. 1, p. 247-252, 2013.
dc.identifier.doi10.1016/j.lwt.2012.05.022
dc.identifier.file2-s2.0-84866391006.pdf
dc.identifier.issn0023-6438
dc.identifier.lattes8104143593771412
dc.identifier.scopus2-s2.0-84866391006
dc.identifier.urihttp://hdl.handle.net/11449/74181
dc.identifier.wosWOS:000309896600034
dc.language.isoeng
dc.relation.ispartofLWT: Food Science and Technology
dc.relation.ispartofjcr3.129
dc.relation.ispartofsjr1,339
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectBrassicaceae
dc.subjectGlucotropaeolin
dc.subjectHPLC
dc.subjectOrganic cultivation
dc.subjectTotal glucosinolates
dc.subjectChronic disease
dc.subjectGlucosinolates
dc.subjectOrganic products
dc.subjectPlant secondary metabolites
dc.subjectProduction methods
dc.subjectProduction system
dc.subjectTrifluoroacetic acids
dc.subjectChemical contamination
dc.subjectPlants (botany)
dc.subjectVegetables
dc.subjectBrassica oleracea var. italica
dc.titleAnalysis of total glucosinolates and chromatographically purified benzylglucosinolate in organic and conventional vegetablesen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
unesp.author.lattes8104143593771412
unesp.author.orcid0000-0002-4874-7205[3]
unesp.author.orcid0000-0002-1792-2605[4]
unesp.author.orcid0000-0001-9480-4146[2]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt

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