Role of annexin 1 gene expression in mouse craniofacial bone development

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dc.contributor.authorDamazo, Amilcar Sabino
dc.contributor.authorMoradi-Bidhendi, Niloufar
dc.contributor.authorOliani, Sonia Maria [UNESP]
dc.contributor.authorFlower, Roderick John
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionWilliam Harvey Research Institute
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionQM School of Medicine and Dentistry
dc.date.accessioned2014-05-27T11:22:30Z
dc.date.available2014-05-27T11:22:30Z
dc.date.issued2007-07-01
dc.description.abstractBACKGROUND: Annexin 1 is a 37-kDa protein that has complex intra- and extracellular effects. To discover whether the absence of this protein alters bone development, we monitored this event in the annexin-A1 null mice in comparison with littermate wild-type controls. METHODS: Radiographic and densitometry methods were used for the assessment of bone in annexin-A1 null mice at a gross level. We used whole-skeleton staining, histological analysis, and Western blotting techniques to monitor changes at the tissue and cellular levels. RESULTS: There were no gross differences in the appendicular skeleton between the genotypes, but an anomalous development of the skull was observed in the annexin-A1 null mice. This was characterized in the newborn annexin-A1 null animals by a delayed intramembranous ossification of the skull, incomplete fusion of the interfrontal suture and palatine bone, and the presence of an abnormal suture structure. The annexin-A1 gene was shown to be active in osteocytes during this phase and COX-2 was abundantly expressed in cartilage and bone taken from annexin-A1 null mice. CONCLUSIONS: Expression of the annexin-A1 gene is important for the normal development of the skull in mice, possibly through the regulation of osteoblast differentiation and a secondary effect on the expression of components of the cPLA2-COX-2 system. © 2007 Wiley-Liss, Inc.en
dc.description.affiliationPost-Graduation in Morphology Federal University of São Paulo (UNIFESP) Paulista School of Medicine (EPM), São Paulo
dc.description.affiliationCentre of Biochemical Pharmacology William Harvey Research Institute, Charterhouse Square, London
dc.description.affiliationDepartment of Biology University of São Paolo State, S. José do Rio Preto, SP
dc.description.affiliationBiomaterials in Relation to Dentistry QM School of Medicine and Dentistry, London
dc.description.affiliationBiomaterials in Relation to Dentistry Francis Bancroft Building QM School of Medicine and Dentistry, Mile End Road, London, E1 4NS
dc.format.extent524-532
dc.identifierhttp://dx.doi.org/10.1002/bdra.20368
dc.identifier.citationBirth Defects Research Part A - Clinical and Molecular Teratology, v. 79, n. 7, p. 524-532, 2007.
dc.identifier.doi10.1002/bdra.20368
dc.identifier.issn1542-0752
dc.identifier.issn1542-0760
dc.identifier.lattes5102737730539655
dc.identifier.scopus2-s2.0-34447504023
dc.identifier.urihttp://hdl.handle.net/11449/69728
dc.language.isoeng
dc.relation.ispartofBirth Defects Research Part A - Clinical and Molecular Teratology
dc.relation.ispartofjcr1.603
dc.relation.ispartofsjr0,852
dc.relation.ispartofsjr0,852
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectAnnexin 1
dc.subjectBone
dc.subjectCyclooxygenase-2
dc.subjectCytosolic phospholipase A2
dc.subjectOsteoblast
dc.subjectannexin
dc.subjectannexin 1
dc.subjectcyclooxygenase 2
dc.subjectphospholipase A2
dc.subjectprotein
dc.subjectunclassified drug
dc.subjectadolescent
dc.subjectanimal tissue
dc.subjectannexin a1 gene
dc.subjectbone density
dc.subjectcontrolled study
dc.subjectcraniofacial development
dc.subjectfemale
dc.subjectgene
dc.subjectgene activation
dc.subjecthistology
dc.subjectimmunohistochemistry
dc.subjectmale
dc.subjectmouse
dc.subjectnewborn
dc.subjectnonhuman
dc.subjectossification
dc.subjectosteocyte
dc.subjectpriority journal
dc.subjectprotein expression
dc.subjectskull suture
dc.subjectWestern blotting
dc.subjectAnimals
dc.subjectAnimals, Newborn
dc.subjectAnnexin A1
dc.subjectBone and Bones
dc.subjectBone Density
dc.subjectBone Development
dc.subjectCraniofacial Abnormalities
dc.subjectCyclooxygenase 2
dc.subjectFemale
dc.subjectGene Expression
dc.subjectHomozygote
dc.subjectMale
dc.subjectMice
dc.subjectMice, Inbred C57BL
dc.subjectMice, Knockout
dc.subjectOsteogenesis
dc.subjectPhospholipases A
dc.subjectAnimalia
dc.subjectMus
dc.titleRole of annexin 1 gene expression in mouse craniofacial bone developmenten
dc.typeArtigo
dcterms.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
unesp.author.lattes5102737730539655
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências Letras e Ciências Exatas, São José do Rio Pretopt

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