Exercise training improves relaxation response and SOD-1 expression in aortic and mesenteric rings from high caloric diet-fed rats

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dc.contributor.authorMoraes, Camila de
dc.contributor.authorDavel, Ana Paula Couto
dc.contributor.authorRossoni, Luciana Venturini
dc.contributor.authorAntunes, Edson
dc.contributor.authorZanesco, Angelina
dc.contributor.institutionUniversidade Cruzeiro do Sul
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-27T11:23:36Z
dc.date.available2014-05-27T11:23:36Z
dc.date.issued2008-07-14
dc.description.abstractBackground. Obesity has been associated with a variety of disease such as type II diabetes mellitus, arterial hypertension and atherosclerosis. Evidences have shown that exercise training promotes beneficial effects on these disorders, but the underlying mechanisms are not fully understood. The aim of this study was to investigate whether physical preconditioning prevents the deleterious effect of high caloric diet in vascular reactivity of rat aortic and mesenteric rings. Methods. Male Wistar rats were divided into sedentary (SD); trained (TR); sedentary diet (SDD) and trained diet (TRD) groups. Run training (RT) was performed in sessions of 60 min, 5 days/week for 12 weeks (70-80% VO2max). Triglycerides, glucose, insulin and nitrite/nitrate concentrations (NOx -) were measured. Concentration- response curves to acetylcholine (ACh) and sodium nitroprusside (SNP) were obtained. Expression of Cu/Zn superoxide dismutase (SOD-1) was assessed by Western blotting. Results. High caloric diet increased triglycerides concentration (SDD: 216 ± 25 mg/dl) and exercise training restored to the baseline value (TRD: 89 ± 9 mg/dl). Physical preconditioning significantly reduced insulin levels in both groups (TR: 0.54 ± 0.1 and TRD: 1.24 ± 0.3 ng/ml) as compared to sedentary animals (SD: 0.87 ± 0.1 and SDD: 2.57 ± 0.3 ng/ml). On the other hand, glucose concentration was slightly increased by high caloric diet, and RT did not modify this parameter (SD: 126 ± 6; TR: 140 ± 8; SDD: 156 ± 8 and TRD 153 ± 9 mg/dl). Neither high caloric diet nor RT modified NO x - levels (SD: 27 ± 4; TR: 28 ± 6; SDD: 27 ± 3 and TRD: 30 ± 2 μM). Functional assays showed that high caloric diet impaired the relaxing response to ACh in mesenteric (about 13%), but not in aortic rings. RT improved the relaxing responses to ACh either in aortic (28%, for TR and 16%, to TRD groups) or mesenteric rings (10%, for TR and 17%, to TRD groups) that was accompanied by up-regulation of SOD-1 expression and reduction in triglycerides levels. Conclusion. The improvement in endothelial function by physical preconditioning in mesenteric and aortic arteries from high caloric fed-rats was directly related to an increase in NO bioavailability to the smooth muscle mostly due to SOD-1 up regulation. © 2008 de Moraes et al; licensee BioMed Central Ltd.en
dc.description.affiliationBiological Science and Health Faculty of Physical Education Cruzeiro Do Sul University, São Paulo (SP)
dc.description.affiliationDepartment of Physiology and Biophysics Institute of Biomedical Sciences (ICB-I) University of São Paulo, São Paulo (SP)
dc.description.affiliationDepartment of Pharmacology Faculty of Medical Sciences University of Campinas, Campinas (SP)
dc.description.affiliationDepartment of Physical Education Institute of Bioscience University of Sao Paulo State, Rio Claro (SP)
dc.identifierhttp://dx.doi.org/10.1186/1472-6793-8-12
dc.identifier.citationBMC Physiology, v. 8, n. 1, 2008.
dc.identifier.doi10.1186/1472-6793-8-12
dc.identifier.file2-s2.0-46749100655.pdf
dc.identifier.issn1472-6793
dc.identifier.lattes4472007237545596
dc.identifier.scopus2-s2.0-46749100655
dc.identifier.urihttp://hdl.handle.net/11449/70482
dc.language.isoeng
dc.relation.ispartofBMC Physiology
dc.relation.ispartofsjr0,936
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectacetylcholine
dc.subjectcopper zinc superoxide dismutase
dc.subjectglucose
dc.subjectinsulin
dc.subjectnitrate
dc.subjectnitrite
dc.subjectnitroprusside sodium
dc.subjecttriacylglycerol
dc.subjectsuperoxide dismutase
dc.subjectanimal experiment
dc.subjectanimal food
dc.subjectaorta
dc.subjectcaloric intake
dc.subjectcontrol group
dc.subjectcontrolled study
dc.subjectexercise
dc.subjectfood intake
dc.subjectmale
dc.subjectmesentery
dc.subjectnonhuman
dc.subjectrat
dc.subjectrelaxation training
dc.subjectWestern blotting
dc.subjectanimal
dc.subjectfat intake
dc.subjectmesenteric artery
dc.subjectmetabolism
dc.subjectmethodology
dc.subjectphysiology
dc.subjectvascular endothelium
dc.subjectvasodilatation
dc.subjectWistar rat
dc.subjectAnimals
dc.subjectAorta
dc.subjectDietary Fats
dc.subjectEndothelium, Vascular
dc.subjectEnergy Intake
dc.subjectMale
dc.subjectMesenteric Arteries
dc.subjectPhysical Conditioning, Animal
dc.subjectRats
dc.subjectRats, Wistar
dc.subjectSuperoxide Dismutase
dc.subjectVasodilation
dc.titleExercise training improves relaxation response and SOD-1 expression in aortic and mesenteric rings from high caloric diet-fed ratsen
dc.typeArtigo
dcterms.licensehttp://www.biomedcentral.com/about/license
unesp.author.lattes4472007237545596
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Rio Claropt

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