Paracoccidioides brasiliensis Uses Endogenous and Exogenous Arachidonic Acid for PGE(x) Production

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dc.contributor.authorBiondo, Guilherme Augusto [UNESP]
dc.contributor.authorDias-Melicio, Luciane Alarcao [UNESP]
dc.contributor.authorBordon-Graciani, Ana Paula [UNESP]
dc.contributor.authorAcorci-Valerio, Michele Janegitz [UNESP]
dc.contributor.authorSoares, Angela Maria Victoriano Campos [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T13:51:01Z
dc.date.available2014-05-20T13:51:01Z
dc.date.issued2010-08-01
dc.description.abstractParacoccidioides brasiliensis is the agent of paracoccidioidomycosis, the most prevalent deep mycosis in Latin America. Production of eicosanoids during fungal infections plays a critical role on fungal biology as well as on host immune response modulation. The purpose of our study was to assess whether P. brasiliensis strains with different degree of virulence (Pb18, Pb265, Bt79, Pb192) produce prostaglandin E-x (PGE(x)). Moreover, we asked if P. brasiliensis could use exogenous sources of arachidonic acid (AA), as well as metabolic pathways dependent on cyclooxygenase (COX) enzyme, as reported for mammalian cells. A possible association between this prostanoid and fungus viability was also assessed. Our results showed that all strains, independently of their virulence, produce high PGE(x) levels on 4 h culture that were reduced after 8 h. However, in both culture times, higher prostanoid levels were detected after supplementation of medium with exogenous AA. Treatment with indomethacin, a COX inhibitor, induced a reduction on PGEx, as well as in fungus viability. The data provide evidence that P. brasiliensis produces prostaglandin-like molecules by metabolizing either endogenous or exogenous AA. Moreover, the results suggest the involvement of these mediators on fungal viability.en
dc.description.affiliationUNESP, Inst Biociencias, Dept Microbiol & Imunol, BR-18618000 Botucatu, SP, Brazil
dc.description.affiliationUnespUNESP, Inst Biociencias, Dept Microbiol & Imunol, BR-18618000 Botucatu, SP, Brazil
dc.format.extent123-130
dc.identifierhttp://dx.doi.org/10.1007/s11046-010-9301-x
dc.identifier.citationMycopathologia. Dordrecht: Springer, v. 170, n. 2, p. 123-130, 2010.
dc.identifier.doi10.1007/s11046-010-9301-x
dc.identifier.issn0301-486X
dc.identifier.urihttp://hdl.handle.net/11449/18213
dc.identifier.wosWOS:000279477700006
dc.language.isoeng
dc.publisherSpringer
dc.relation.ispartofMycopathologia
dc.relation.ispartofjcr1.476
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectParacoccidioides brasiliensisen
dc.subjectProstaglandin E-xen
dc.subjectFungal survivalen
dc.subjectCyclooxygenaseen
dc.titleParacoccidioides brasiliensis Uses Endogenous and Exogenous Arachidonic Acid for PGE(x) Productionen
dc.typeArtigo
dcterms.licensehttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dcterms.rightsHolderSpringer
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt

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Artigos - Microbiologia e Imunologia - IBB