The extract of the jellyfish Phyllorhiza punctata promotes neurotoxic effects

Página do item simplificado
dc.contributor.authorVasconcelos Carneiro, Raquel Felipe
dc.contributor.authorFalcao do Nascimento, Nilberto Robson
dc.contributor.authorCorreia Costa, Paula Priscila
dc.contributor.authorGomes, Victor Martins
dc.contributor.authorFelizardo de Souza, Alex Jardelino [UNESP]
dc.contributor.authorBuzzo de Oliveira, Simone Cristina [UNESP]
dc.contributor.authordos Santos Diz Filho, Eduardo Britto [UNESP]
dc.contributor.authorZara, Fernando Jose [UNESP]
dc.contributor.authorFonteles, Manasses Claudino
dc.contributor.authorToyama, Daniela de Oliveira
dc.contributor.authorToyama, Marcos Hikari [UNESP]
dc.contributor.authorSantos, Claudia Ferreira
dc.contributor.institutionUniv Estadual Ceara
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.contributor.institutionUniv Mackenzie
dc.date.accessioned2014-05-20T13:13:15Z
dc.date.available2014-05-20T13:13:15Z
dc.date.issued2011-11-01
dc.description.abstractPhyllorhiza punctata (P. punctata) is a jellyfish native to the southwestern Pacific. Herewith we present the biochemical and pharmacological characterization of an extract of the tentacles of P. punctata. The tentacles were subjected to three freezethaw cycles, homogenized, ultrafiltered, precipitated, centrifuged and lyophilized to obtain a crude extract (PHY-N). Paralytic shellfish poisoning compounds such as saxitoxin, gonyautoxin-4, tetrodotoxin and brevetoxin-2, as well as several secretory phospholipase A2 were identified. PHY-N was tested on autonomic and somatic neuromuscular preparations. In mouse vas deferens, PHY-N induced phasic contractions that reached a peak of 234 +/- 34.7% of control twitch height, which were blocked with either 100 mu m of phentolamine or 1m m of lidocaine. In mouse corpora cavernosa, PHY-N evoked a relaxation response, which was blocked with either L-NG-Nitroarginine methyl ester (0.5 m m) or 1m m of lidocaine. PHY-N (1, 3 and 10 mu g ml(-1)) induced an increase in tonus of the biventercervicis neuromuscular preparation that was blocked with pre-treatment of galamine (10 mu m). Administration of 6 mg kg(-1) PHY-N intramuscularly produced death in broilers by spastic paralysis. In conclusion, PHY-N induces nerve depolarization and nonspecifically increases neurotransmitter release. Copyright (C) 2011 John Wiley & Sons, Ltd.en
dc.description.affiliationUniv Estadual Ceara, Super Inst Biomed, Fortaleza, Ceara, Brazil
dc.description.affiliationUniv Estadual Paulista, São Paulo, Brazil
dc.description.affiliationUniv Estadual Campinas, Dept Bioquim, Inst Biol, Campinas, SP, Brazil
dc.description.affiliationUniv Mackenzie, Ctr Ciencias Biol & Saude, São Paulo, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, São Paulo, Brazil
dc.description.sponsorshipFundação Cearense de Apoio ao Desenvolvimento Científico e Tecnológico (FUNCAP)
dc.format.extent720-729
dc.identifierhttp://dx.doi.org/10.1002/jat.1620
dc.identifier.citationJournal of Applied Toxicology. Malden: Wiley-blackwell, v. 31, n. 8, p. 720-729, 2011.
dc.identifier.doi10.1002/jat.1620
dc.identifier.issn0260-437X
dc.identifier.lattes8573195327542061
dc.identifier.urihttp://hdl.handle.net/11449/1102
dc.identifier.wosWOS:000297843800003
dc.language.isoeng
dc.publisherWiley-Blackwell
dc.relation.ispartofJournal of Applied Toxicology
dc.relation.ispartofjcr2.909
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectNeurotoxicityen
dc.subjectmarine neurotoxic alkaloidsen
dc.subjectneuromuscular blockadeen
dc.subjectsmooth muscle spasmen
dc.subjectspastic paralysisen
dc.subjectnitrergic stimulationen
dc.titleThe extract of the jellyfish Phyllorhiza punctata promotes neurotoxic effectsen
dc.typeArtigo
dcterms.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dcterms.rightsHolderWiley-blackwell
unesp.author.lattes8573195327542061
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, São Vicentept
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Agrárias e Veterinárias, Jaboticabalpt

Arquivos

Licença do Pacote
Agora exibindo 1 - 2 de 2
Nenhuma Miniatura disponível
Nome:
license.txt
Tamanho:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descrição:
Nenhuma Miniatura disponível
Nome:
license.txt
Tamanho:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descrição:

Coleções

Artigos - Biologia - FCAV
Artigos - Ciências Biológicas - São Vicente