Impact of sub-inhibitory concentrations of amoxicillin on helicobacter pylori virulence factors

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dc.contributor.authorDonofrio, Fabian C. [UNESP]
dc.contributor.authorRaddi, Maria Stella Gonçalves [UNESP]
dc.contributor.authorCarlos, Iracilda Zeppone [UNESP]
dc.contributor.authorMiranda, Elaine T. [UNESP]
dc.contributor.authorMaia, Danielle C. G. [UNESP]
dc.contributor.authorFerrezini, Jenifer
dc.contributor.authorFioramonte, Mariana
dc.contributor.authorCarrilho, Emanuel
dc.contributor.authorGozzo, Fabio Cesar
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Federal de São Carlos (UFSCar)
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.date.accessioned2016-01-28T16:56:17Z
dc.date.available2016-01-28T16:56:17Z
dc.date.issued2014
dc.description.abstractHelicobacter pylori are etiological factor of human acute and chronic gastritis. Critical host responses that influence the progression to H. pylori-induced carcinogenesis include gastric inflammation. This study was conducted to evaluate whether subinhibitory concentrations of amoxicillin can affect H. pylori-induced cytokines by RAW 264.7 macrophage-like cells. In addition, a proteomic approach was used to compare H. pylori protein expression profiles under amoxicillin treatment. H. pylori ATCC 43504 was cultured with or without amoxicillin at subinhibitory concentrations (sub-MICs). The cytokines (IL 1-β, IL-6, IL-12, and TNF-α) released by infected RAW 264.7 cells were quantified by a sandwich immunoassay. Differential protein analysis of H. pylori was performed using 2-dimensional electrophoresis (2-DE) followed by mass spectrometry and the sequences were searched against the SWISS-PROT data bank. Pre-treatment H. pylori with sub-MIC of amoxicillin increased IL-1β and IL-12 production by RAW 264.7 cells. Proteomics analyses of proteins revealed that the sub-MIC treatment affected the expression of several classes of proteins. Among the nine most over expressed proteins included heat shock proteins, superoxide dismutase, enolase, ATP synthase alpha subunit, neutrophil activating protein and argininosuccinate synthase. The results shown in the present work indicate that sub-MICs of amoxicillin can induce specific changes on the cell wall proteins and behavior of susceptible H. pylori that can contribute to the pathogenesis of the bacterium.en
dc.description.affiliationUniversidade Estadual Paulista Júlio de Mesquita Filho, Departamento de Análises Clínicas, Faculdade de Ciências Farmacêuticas de Araraquara, Araraquara, Rua Expedicionários do Brasil, 1621, Centro, CEP 14801902, SP, Brasil
dc.description.affiliationGrupo de Bioanalítica, Microfabricação e Separações, Instituto de Química de São Carlos, Universidade de São Paulo, São Carlos, SP, Brazil
dc.description.affiliationInstituto de Química, Universidade Estadual de Campinas, Campinas, SP, Brazil
dc.description.affiliationInstituto Nacional de Ciência e Tecnologia de Bioanalítica, Campinas, SP, Brazil
dc.description.affiliationUnespUniversidade Estadual Paulista Júlio de Mesquita Filho, Departamento de Análises Clínicas, Faculdade de Ciências Farmacêuticas de Araraquara, Araraquara, Rua Expedicionários do Brasil, 1621, Centro, CEP 14801902, SP, Brasil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 2009/1598-7
dc.format.extent30-46
dc.identifierhttp://www.wjpr.net/dashboard/abstract_id/1103
dc.identifier.citationWorld Journal of Pharmaceutical Research, v. 3, n. 5, p. 30-46, 2014.
dc.identifier.issn2277-7105
dc.identifier.lattes1730146818754269
dc.identifier.lattes4064204116589015
dc.identifier.urihttp://hdl.handle.net/11449/133711
dc.language.isoeng
dc.relation.ispartofWorld Journal of Pharmaceutical Research
dc.rights.accessRightsAcesso restrito
dc.sourceCurrículo Lattes
dc.subjectHelicobacter pylorien
dc.subjectAmoxicillinen
dc.subjectSub-inhibitory concentrationen
dc.subjectCytokinesen
dc.subjectMacrophageen
dc.subjectProteomicsen
dc.titleImpact of sub-inhibitory concentrations of amoxicillin on helicobacter pylori virulence factorsen
dc.typeArtigo
unesp.author.lattes1730146818754269
unesp.author.lattes4064204116589015
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Farmacêuticas, Araraquarapt
unesp.departmentAnálises Clínicaspt

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