Purification and characterization of jararassin-I, a thrombin-like enzyme from Bothrops jararaca snake venom

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dc.contributor.authorVieira, D. F.
dc.contributor.authorWatanabe, L.
dc.contributor.authorSant'ana, C. D.
dc.contributor.authorMarcussi, S.
dc.contributor.authorSampaio, S. V.
dc.contributor.authorSoares, A. M.
dc.contributor.authorArni, R. K.
dc.contributor.institutionUniv Ribeirao Preto
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2014-05-20T15:27:14Z
dc.date.available2014-05-20T15:27:14Z
dc.date.issued2004-12-01
dc.description.abstractA thrombin-like serine protease, jararassin-I, was isolated from the venom of Bothrops jararaca. The protein was obtained in high yield and purity by a single chromatographic step using the affinity resin Benzamidine-Sepharose CL-6B. SDS-PAGE and dynamic light scattering analyses indicated that the molecular mass of the enzyme was about 30 kD. The enzyme possessed fibrinogenolytic and coagulant activities. The jararassin-I degraded the Bbeta chain of fibrinogen while the Aalpha chain and gammachain were unchanged. Proteases inhibitors, PMSF and benzamidine inhibited the coagulant activity. These results showed jararassin-I is a serine protease similar to coagulating thrombin-like snake venom proteases, but it specifically cleaves Bbeta chain of bovine fibrinogen. Single crystals of enzyme were obtained (0.2 mmx0.2 mmx0.2 mm) and used for X-ray diffraction experiments.en
dc.description.affiliationUniv Ribeirao Preto, Unidade Biotecnol, Ribeirao Preto, SP, Brazil
dc.description.affiliationUniv Estadual Paulista, Inst Biociencias Letras & Ciências Exactas, Dept Fis, UNESP, BR-15054000 Sao Jose do Rio Preto, SP, Brazil
dc.description.affiliationUniv São Paulo, FCFRP, Dept Anal Clin Toxicol & Bromatol, BR-14049 Ribeirao Preto, SP, Brazil
dc.description.affiliationUniv São Paulo, FMRP, Dept Bioquim & Imunol, BR-14049 Ribeirao Preto, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Inst Biociencias Letras & Ciências Exactas, Dept Fis, UNESP, BR-15054000 Sao Jose do Rio Preto, SP, Brazil
dc.format.extent798-802
dc.identifierhttp://dx.doi.org/10.1093/abbs/36.12.798
dc.identifier.citationActa Biochimica Et Biophysica Sinica. Shanghai: Shanghai Inst Biochemistry, Academia Sinica, v. 36, n. 12, p. 798-802, 2004.
dc.identifier.doi10.1093/abbs/36.12.798
dc.identifier.issn1672-9145
dc.identifier.lattes9162508978945887
dc.identifier.orcid0000-0003-2460-1145
dc.identifier.urihttp://hdl.handle.net/11449/37257
dc.identifier.wosWOS:000225986000003
dc.language.isoeng
dc.publisherShanghai Inst Biochemistry, Academia Sinica
dc.relation.ispartofActa Biochimica et Biophysica Sinica
dc.relation.ispartofjcr2.224
dc.relation.ispartofsjr0,790
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectsnake venompt
dc.subjectBothrops jararacapt
dc.subjectserine protease thrombin-likept
dc.subjectfibrinogenolytic activitypt
dc.subjectcrystallizationpt
dc.titlePurification and characterization of jararassin-I, a thrombin-like enzyme from Bothrops jararaca snake venomen
dc.typeArtigo
dcterms.licensehttp://www.oxfordjournals.org/access_purchase/self-archiving_policyb.html
dcterms.rightsHolderShanghai Inst Biochemistry, Academia Sinica
unesp.author.lattes9162508978945887[7]
unesp.author.orcid0000-0003-2460-1145[7]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências Letras e Ciências Exatas, São José do Rio Pretopt

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