High fat-induced obesity associated with insulin-resistance increases FGF-2 content and causes stromal hyperplasia in rat ventral prostate

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dc.contributor.authorRibeiro, Daniele Lisboa
dc.contributor.authorPinto, Maria Etelvina [UNESP]
dc.contributor.authorMaeda, Samantha Yuri [UNESP]
dc.contributor.authorTaboga, Sebastiao Roberto [UNESP]
dc.contributor.authorGoes, Rejane Maira [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Federal de Uberlândia (UFU)
dc.date.accessioned2014-05-20T14:01:06Z
dc.date.available2014-05-20T14:01:06Z
dc.date.issued2012-08-01
dc.description.abstractObesity affects sex hormone secretion, which can negatively influence prostatic structure, homeostasis, and disease. This investigation aimed to evaluate the repercussions of obesity induced by a high-fat diet on the rat prostate, with or without treatment with the aromatase inhibitor, Letrozole. Adult Wistar rats were fed a high-fat diet (20% saturated fat, O) for 15 weeks to induce obesity or received a balanced diet (4% fat, C). Then, a group of C and O rats were daily treated with Letrozole (1 mg/kg b.w. per day) for 2 weeks (CL and OL, respectively). Subsequently, ventral prostate was processed for analysis by transmission electron microscopy, immunohistochemistry, and Western blotting. Obesity decreased 70% of the testosterone plasma level. The prostate showed epithelial atrophy and dilated acini in the intermediate portion and epithelial wrinkling in the distal tips. The relative frequency of smooth muscle alpha-actin in the O group increased by 67%. Ultrastructurally, epithelial cells in obese animals presented altered secretory organelles, lipid droplets, and thicker subjacent fibromuscular layer. Letrozole treatment caused a partial restoration of the prostatic changes caused by obesity. Obesity increased the prostatic content of fibroblast growth factor-2 (FGF-2) by 150%, and Letrozole treatment increased this protein even more in the control and obese groups. This investigation shows that obesity provokes structural and ultrastructural changes in the epithelium of rat prostate; these changes might affect gland homeostasis and physiology. The epithelial and smooth muscle cell hyperplasia and increased FGF-2 expression observed in this experimental model of obesity/insulin-resistance might explain the high frequency of benign prostatic hyperplasia in insulin-resistant men.en
dc.description.affiliationIBILCE Unesp, Dept Biol, BR-15054000 São Paulo, Brazil
dc.description.affiliationFed Univ Uberlandia UFU, Inst Biomed Sci, Dept Histol, Uberlandia, MG, Brazil
dc.description.affiliationUniv Estadual Paulista UNESP, Dept Biol, Inst Biosci Letters & Exact Sci, São Paulo, Brazil
dc.description.affiliationUnespIBILCE Unesp, Dept Biol, BR-15054000 São Paulo, Brazil
dc.description.affiliationUnespUniv Estadual Paulista UNESP, Dept Biol, Inst Biosci Letters & Exact Sci, São Paulo, Brazil
dc.format.extent577-588
dc.identifierhttp://dx.doi.org/10.1007/s00441-012-1420-x
dc.identifier.citationCell and Tissue Research. New York: Springer, v. 349, n. 2, p. 577-588, 2012.
dc.identifier.doi10.1007/s00441-012-1420-x
dc.identifier.issn0302-766X
dc.identifier.lattes1445259468526188
dc.identifier.lattes0947193347312157
dc.identifier.orcid0000-0002-0970-4288
dc.identifier.orcid0000-0002-3622-460X
dc.identifier.urihttp://hdl.handle.net/11449/21591
dc.identifier.wosWOS:000307401200015
dc.language.isoeng
dc.publisherSpringer
dc.relation.ispartofCell and Tissue Research
dc.relation.ispartofjcr3.043
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectProstateen
dc.subjectObesityen
dc.subjectSmooth muscle cellsen
dc.subjectBenign prostatic hyperplasiaen
dc.subjectFibroblast growth factoren
dc.subjectRat (Wistar)en
dc.titleHigh fat-induced obesity associated with insulin-resistance increases FGF-2 content and causes stromal hyperplasia in rat ventral prostateen
dc.typeArtigo
dcterms.licensehttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dcterms.rightsHolderSpringer
unesp.author.lattes1445259468526188
unesp.author.lattes0947193347312157
unesp.author.lattes0947193347312157[5]
unesp.author.orcid0000-0002-2205-0864[2]
unesp.author.orcid0000-0002-3622-460X[5]
unesp.author.orcid0000-0002-0970-4288[4]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências Letras e Ciências Exatas, São José do Rio Pretopt

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