In vitro evaluation of the cytotoxicity and eye irritation potential of preservatives widely used in cosmetics

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dc.contributor.authorCorrêa, Gabriela de Oliveira Prado [UNESP]
dc.contributor.authorMarcato, Danieli Camilo [UNESP]
dc.contributor.authorRamos, Wagner Soares [UNESP]
dc.contributor.authorCorrêa, Marcos Antônio [UNESP]
dc.contributor.authorCicarelli, Regina Maria Barretto [UNESP]
dc.contributor.authorIsaac, Vera Lucia Borges [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.date.accessioned2023-07-29T15:13:02Z
dc.date.available2023-07-29T15:13:02Z
dc.date.issued2022-01-01
dc.description.abstractThe consumption of cosmetics has been increasing every year and is expected to reach $675 billion by 2020 at an estimated growth rate of 6.4% per year. Exposure to skin irritants is the major cause of non-immunological inflammation of the skin. Therefore, the safety evaluation of cosmetic preservatives should be increased. Thus, the present work aimed to evaluate the cytotoxicity as the viability endpoint and the eye irritation potential of preservatives used in cosmetics. Cytotoxicity assays were performed using MTT and NRU in human keratinocytes (HaCaT), human dermal fibroblasts, adult (HDFa), and human hepatoma cells (HepG2). The eye irritation potential was evaluated using the Hen’s Egg Test-chorioallantoic membrane (HET-CAM). The evaluated preservatives were methylparaben (MP), propylparaben (PP), phenoxyethanol (PE), and a mixture of methylchloroisothiazolinone and methylisothiazolinone (CMI/MI). All preservatives showed cytotoxic potential within the permitted concentrations for use in cosmetic products. In the HET-CAM test, PE and CMI/MI, MP, and PP were classified as severe, moderate, and poor irritants, respectively. Our results indicate that proper safety evaluations are required to ensure the beneficial properties of preservatives on cosmetic products without exceeding exposure levels that would result in adverse health effects for consumers.en
dc.description.affiliationSão Paulo State University (UNESP) School of Pharmaceutical Sciences
dc.description.affiliationUnespSão Paulo State University (UNESP) School of Pharmaceutical Sciences
dc.description.sponsorshipUniversidade Estadual Paulista
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipIdCAPES: 001
dc.identifierhttp://dx.doi.org/10.1590/s2175-97902022e20039
dc.identifier.citationBrazilian Journal of Pharmaceutical Sciences, v. 58.
dc.identifier.doi10.1590/s2175-97902022e20039
dc.identifier.issn2175-9790
dc.identifier.issn1984-8250
dc.identifier.scopus2-s2.0-85141128145
dc.identifier.urihttp://hdl.handle.net/11449/249325
dc.language.isoeng
dc.relation.ispartofBrazilian Journal of Pharmaceutical Sciences
dc.sourceScopus
dc.subjectCell culture
dc.subjectCosmetics
dc.subjectCytotoxicity
dc.subjectHET-CAM test
dc.subjectPreservatives
dc.titleIn vitro evaluation of the cytotoxicity and eye irritation potential of preservatives widely used in cosmeticsen
dc.typeArtigo
unesp.author.orcid0000-0001-7402-920X[6]

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Artigos - Fármacos e Medicamentos - FCFAR
Artigos - Ciências Biológicas - FCFAR