Proteinograma sérico de bezerros recém-nascidos da raça Holandesa obtido por eletroforese em gel de poliacrilamida

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dc.contributor.authorFagliari, J.J. [UNESP]
dc.contributor.authorRizolli, F.W.
dc.contributor.authorSilva, S.L. [UNESP]
dc.contributor.authorSilva, D.G. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T13:14:07Z
dc.date.available2014-05-20T13:14:07Z
dc.date.issued2006-06-01
dc.description.abstractThe serum protein concentration of newborn Holstein calves determined by means of sodium dodecyl sulphate-polyacrylamide (SDS-PAGE) was studied. Blood samples from 40 healthy newborn calves were obtained 48 hours after birth. Calves had been given 3 liters of colostrum within 2 hours after birth, following by dose corresponding by 15% of animal weight each 24 hours. The results showed three different proteinograms: 19 calves had 14 proteins with molecular weights (MW) ranging from 28,000 D to 170,000D (proteinogram 1); 11 calves had 14 proteins with MW ranging from 18,000 to 170,000 D (proteinogram 1); and 10 calves had 12 proteins with MW ranging from 28,000 D to 170,000 D (proteinogram 3). The three groups presented similar IgG levels. The highest serum concentration of ceruloplasmin were verified in proteinogram 3, which had the lowest serum level of protein with MW 58,000D. It was verified a1-antitrypsin only in proteinogram 2, which had no proteins with MW of 42,000 D and 37,000D. The highest serum concentrations of IgA and protein with MW 58,000 D, and the lowest serum levels of transferrin, haptoglobin, and acid glycoprotein were verified in proteinogram 3. Measurement of serum protein concentrations by SDS-PAGE may be useful in monitoring the occurrence of hypogammaglobulinemia and the neonatal disease in calves.en
dc.description.affiliationUNESP Faculdade de Ciências Agrárias e Veterinárias
dc.description.affiliationUNESP FCAV
dc.description.affiliationUnespUNESP Faculdade de Ciências Agrárias e Veterinárias
dc.description.affiliationUnespUNESP FCAV
dc.format.extent450-453
dc.identifierhttp://dx.doi.org/10.1590/S0102-09352006000300026
dc.identifier.citationArquivo Brasileiro de Medicina Veterinária e Zootecnia. Universidade Federal de Minas Gerais, Escola de Veterinária, v. 58, n. 3, p. 450-453, 2006.
dc.identifier.doi10.1590/S0102-09352006000300026
dc.identifier.fileS0102-09352006000300026.pdf
dc.identifier.issn0102-0935
dc.identifier.scieloS0102-09352006000300026
dc.identifier.urihttp://hdl.handle.net/11449/1686
dc.language.isopor
dc.publisherUniversidade Federal de Minas Gerais (UFMG), Escola de Veterinária
dc.relation.ispartofArquivo Brasileiro de Medicina Veterinária e Zootecnia
dc.relation.ispartofjcr0.286
dc.relation.ispartofsjr0,248
dc.rights.accessRightsAcesso aberto
dc.sourceSciELO
dc.subjectCalfen
dc.subjectProteinogramen
dc.subjectElectrophoresisen
dc.subjectSDS-PAGEen
dc.subjectBezerropt
dc.subjectProteinogramapt
dc.subjectEletroforesept
dc.subjectSDS-PAGEpt
dc.titleProteinograma sérico de bezerros recém-nascidos da raça Holandesa obtido por eletroforese em gel de poliacrilamidapt
dc.title.alternativeSerum protein concentration in newborn Holstein calves determined by means of sodium dodecyl sulphate-polyacrylamide gel electrophoresisen
dc.typeArtigo
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Agrárias e Veterinárias, Jaboticabalpt

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Artigos - Clínica e Cirurgia Veterinária - FCAV