Cryogenic preservation of embryos of Prochilodus lineatus (Valenciennes, 1836) (Characiforme; Prochilodontidae)

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dc.contributor.authorNinhaus-Silveira, Alexandre
dc.contributor.authorForesti, Fausto [UNESP]
dc.contributor.authorde Azevedo, Alexandre
dc.contributor.authorAgostinho, Claudio Angelo [UNESP]
dc.contributor.authorVerissimo-Silveira, Rosicleire
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Federal do Rio de Janeiro (UFRJ)
dc.date.accessioned2014-05-20T13:52:31Z
dc.date.available2014-05-20T13:52:31Z
dc.date.issued2009-02-01
dc.description.abstractWhile the freezing techniques of mammal embryos have been providing promising results, the cryopreservation of teleostean eggs and embryos have remained unsuccessful up to now. Therefore, this work aimed to develop a procedure of cryogenic preservation of embryos of Prochilodus lineatus and to observe, at both structural and ultrastructural levels, the morphological alterations that took place after the application of freezing/thawing techniques. The embryos at the morula stage could not tolerate exposure to the cryoprotectants ethylene glycol monomethyl ether, propylene glycol monomethyl ether, methanol, dimethyl sulphoxide and propylene glycol, presenting 100% of mortality. Embryos at the 4- to 6-somites stage tolerated exposure to propylene glycol and dimethyl sulphoxide, and the results revealed no significant differences (alpha = 0.05) regarding survival from both treatments. None of the freezing, thawing and hydration protocols was effective on preserving embryo viability. The ultrastructural analyses of frozen and thawed embryos showed that cells from ectoderm, somites, notochord and endoderm were structurally intact, with well preserved nuclei and mitochondria. The yolk globules were able to tolerate the freezing process, but the yolk syncytial layer was unorganized, displaying an electron-dense and compacted appearance, collapsed reticules, nuclei with modified chromatin and ruptures on the plasmatic membrane at the contact zone with endoderm. It might be concluded that the procedures tested for freezing were unable to avoid the formation of intracellular ice crystals, leading to drastic morphological modifications and making P. lineatus embryos unviable.en
dc.description.affiliationUniv Estadual Paulista, Dept Morfol, Dept Biol & Zootecnia, Botucatu, SP, Brazil
dc.description.affiliationUniv Estadual Paulista, Dept Prod & Exploracao Anim, São Paulo, Brazil
dc.description.affiliationUniv Fed Rio de Janeiro, Nucl Pesquisas Ecol & Desenvolvimento Socio Ambie, Macae, RJ, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Dept Morfol, Dept Biol & Zootecnia, Botucatu, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Dept Prod & Exploracao Anim, São Paulo, Brazil
dc.format.extent45-55
dc.identifierhttp://dx.doi.org/10.1017/S0967199408004991
dc.identifier.citationZygote. New York: Cambridge Univ Press, v. 17, n. 1, p. 45-55, 2009.
dc.identifier.doi10.1017/S0967199408004991
dc.identifier.fileWOS000263836800005.pdf
dc.identifier.issn0967-1994
dc.identifier.lattes0804793944846367
dc.identifier.lattes2772751144347110
dc.identifier.orcid0000-0003-0355-0553
dc.identifier.urihttp://hdl.handle.net/11449/18753
dc.identifier.wosWOS:000263836800005
dc.language.isoeng
dc.publisherCambridge University Press
dc.relation.ispartofZygote
dc.relation.ispartofjcr1.114
dc.relation.ispartofsjr0,387
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectCryopreservationen
dc.subjectEmbryoen
dc.subjectProchilodus lineatusen
dc.subjectToxicityen
dc.titleCryogenic preservation of embryos of Prochilodus lineatus (Valenciennes, 1836) (Characiforme; Prochilodontidae)en
dc.typeArtigo
dcterms.licensehttp://journals.cambridge.org/action/displaySpecialPage?pageId=4676#
dcterms.rightsHolderCambridge Univ Press
unesp.author.lattes0804793944846367
unesp.author.lattes9568814835325380[1]
unesp.author.lattes2772751144347110[4]
unesp.author.orcid0000-0003-0738-0862[1]
unesp.author.orcid0000-0003-0355-0553[4]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina Veterinária e Zootecnia, Botucatupt

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Artigos - Morfologia - IBB
Artigos - Produção Animal - FMVZ