Molecular characterization and clonal diversity of methicillin-susceptible Staphylococcus aureus in milk of cows with mastitis in Brazil

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dc.contributor.authorSilva, N. C C
dc.contributor.authorGuimarães, F. F.
dc.contributor.authorManzi, M. P.
dc.contributor.authorBudri, P. E.
dc.contributor.authorGómez-Sanz, E.
dc.contributor.authorBenito, D.
dc.contributor.authorLangoni, Hélio [UNESP]
dc.contributor.authorRall, V. L M
dc.contributor.authorTorres, C.
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-27T11:30:42Z
dc.date.available2014-05-27T11:30:42Z
dc.date.issued2013-09-19
dc.description.abstractMastitis is an important disease for the dairy industry worldwide, causing economic losses and reducing milk quality and production. Staphylococcus aureus is a worldwide agent of this intramammary infection, which also causes foodborne diseases. The objective of this study was to determine the frequency of methicillin-susceptible Staphylococcus aureus (MSSA) isolates in milk of mastitis cows in Brazil and to analyze the genetic lineages and the content of antimicrobial resistance genes and virulence factors among these isolates. Fifty-six MSSA isolates were recovered from 1,484 milk samples (positive for the California mastitis test) of 518 cows from 11 different farms in Brazil (representing 51% of total Staph. aureus obtained), and they were further characterized. Methicillin-susceptible Staphylococcus aureus were isolated from 3.7% of California mastitis test-positive tested milk samples and from 6.2% of tested mastitic cows. Methicillin-susceptible Staphylococcus aureus isolates were characterized by spa typing, agr typing, and multilocus sequence typing, and resistance and virulence traits were investigated by PCR. Seven spa types were identified among MSSA (% of isolates): t127 (44.6), t605 (37.5), t002, t1784, t2066 (1.8), and 2 new ones: t10856 (10.7) and t10852 (1.8). Five distinct sequence types (ST) were detected (% of isolates): ST1 (46.4), ST126 (37.5), ST133 (10.7), ST5 (3.6), and a novel ST registered as ST2493 (1.8). Resistances were detected for streptomycin, chloramphenicol, and tetracycline. One strain contained the chloramphenicol resistance gene (fexA; included within transposon Tn558) and 3 strains contained the tetracycline resistance gene [tet(K)]. Methicillin-susceptible Staphylococcus aureus strains were susceptible to most of the antibiotics studied and lacked the virulence genes of Panton-Valentine leukocidin (lukF/S-PV), toxic shock syndrome toxin 1 (tst), exfoliative toxin A (eta), and exfoliative toxin B (etb), as well as the genes of the immune evasion cluster. Methicillin-susceptible Staphylococcus aureus isolates were detected in a relatively low proportion of cows with mastitis (6.2%) and recovered isolates presented high diversity of genetic lineages, with CC1 and CC126 the predominant clonal complexes, and CC133 also being detected. Larger epidemiological studies with molecular characterization of isolates are required to deepen the knowledge on the circulating genetic lineages among the cow population with mastitis. © 2013 American Dairy Science Association.en
dc.identifierhttp://dx.doi.org/10.3168/jds.2013-6719
dc.identifier.citationJournal of Dairy Science.
dc.identifier.doi10.3168/jds.2013-6719
dc.identifier.issn0022-0302
dc.identifier.issn1525-3198
dc.identifier.lattes1843683720990222
dc.identifier.scopus2-s2.0-84883861945
dc.identifier.urihttp://hdl.handle.net/11449/76597
dc.identifier.wosWOS:000325664800008
dc.language.isoeng
dc.relation.ispartofJournal of Dairy Science
dc.relation.ispartofjcr2.749
dc.relation.ispartofsjr1,350
dc.relation.ispartofsjr1,350
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectmastitis
dc.subjectmethicillin susceptibility
dc.subjectspa typing
dc.subjectStaphylococcus aureus
dc.titleMolecular characterization and clonal diversity of methicillin-susceptible Staphylococcus aureus in milk of cows with mastitis in Brazilen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
unesp.author.lattes1843683720990222
unesp.author.orcid0000-0003-3349-7431[2]
unesp.author.orcid0000-0001-5127-0762[7]
unesp.author.orcid0000-0003-4280-5619[8]
unesp.author.orcid0000-0002-2839-1416[1]
unesp.author.orcid0000-0003-3709-1690[9]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina Veterinária e Zootecnia, Botucatupt

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Artigos - Higiene Veterinária e Saúde Pública - FMVZ
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