Involvement of pituitary gonadotropins, gonadal steroids and breeding season in sex change of protogynous dusky grouper, Epinephelus marginatus (Teleostei: Serranidae), induced by a non-steroidal aromatase inhibitor

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dc.contributor.authorGarcia, Carlos Eduardo de O.
dc.contributor.authorAraújo, Bruno C.
dc.contributor.authorMello, Paulo H.
dc.contributor.authorNarcizo, Amanda de M.
dc.contributor.authorRodrigues-Filho, Jandyr A. [UNESP]
dc.contributor.authorMedrado, Andreone T.
dc.contributor.authorZampieri, Ricardo A.
dc.contributor.authorFloeter-Winter, Lucile M.
dc.contributor.authorMoreira, Renata Guimarães
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-27T11:30:48Z
dc.date.available2014-05-27T11:30:48Z
dc.date.issued2013-10-01
dc.description.abstractTwo experiments were performed using the aromatase inhibitor (AI) letrozole (100mg/kg) to promote sex change, from female-to-male, in protogynous dusky grouper. One experiment was performed during the breeding season (spring) and the other at the end of the breeding season (summer). During the spring, AI promoted sex change after 9weeks and the sperm produced was able to fertilize grouper oocytes. During the summer, the sex change was incomplete; intersex individuals were present and sperm was not released by any of the animals. Sex changed gonads had a lamellar architecture; cysts of spermatocytes and spermatozoa in the lumen of the germinal compartment. In the spring, after 4weeks, 11ketotestosterone (11KT) levels were higher in the AI than in control fish, and after 9weeks, coincident with semen release, testosterone levels increased in the AI group, while 11KT returned to the initial levels. Estradiol (E2) levels remained unchanged during the experimental period. Instead of decreasing throughout the period, as in control group, 17 α-OH progesterone levels did not change in the AI-treated fish, resulting in higher values after 9weeks when compared with control fish. fshβ and lhβ gene expression in the AI animals were lower compared with control fish after 9weeks. The use of AI was effective to obtain functional males during the breeding season. The increase in androgens, modulated by gonadotropins, triggered the sex change, enabling the development of male germ cells, whereas a decrease in E2 levels was not required to change sex in dusky grouper. © 2013 Elsevier Inc.en
dc.description.affiliationInstituto de Biociências Universidade de São Paulo, Rua do Matão, Trav. 14, 321, São Paulo 05508-090, SP
dc.description.affiliationUniversidade Estadual Paulista Júlio de Mesquita Filho Centro de Aquicultura (CAUNESP), Via de Acesso Prof. Paulo Donato Castelane, s/n., Jaboticabal 14884-900, SP
dc.description.affiliationCentro de Biologia Marinha da Universidade de São Paulo (CEBIMar/USP) Rodovia Manoel Hypólito do Rego, Km. 131, 5, São Sebastião CEP 11600-000. SP
dc.description.affiliationUnespUniversidade Estadual Paulista Júlio de Mesquita Filho Centro de Aquicultura (CAUNESP), Via de Acesso Prof. Paulo Donato Castelane, s/n., Jaboticabal 14884-900, SP
dc.format.extent170-180
dc.identifierhttp://dx.doi.org/10.1016/j.ygcen.2013.06.012
dc.identifier.citationGeneral and Comparative Endocrinology, v. 192, p. 170-180.
dc.identifier.doi10.1016/j.ygcen.2013.06.012
dc.identifier.file2-s2.0-84884416142.pdf
dc.identifier.issn0016-6480
dc.identifier.issn1095-6840
dc.identifier.scopus2-s2.0-84884416142
dc.identifier.urihttp://hdl.handle.net/11449/76738
dc.identifier.wosWOS:000325736400020
dc.language.isoeng
dc.relation.ispartofGeneral and Comparative Endocrinology
dc.relation.ispartofjcr2.564
dc.relation.ispartofsjr1,101
dc.relation.ispartofsjr1,101
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectAromatase inhibitoren
dc.subjectArtificial sex changeen
dc.subjectFollicle-stimulating hormoneen
dc.subjectGonadal steroidsen
dc.subjectLetrozoleen
dc.subjectLuteinizing hormoneen
dc.titleInvolvement of pituitary gonadotropins, gonadal steroids and breeding season in sex change of protogynous dusky grouper, Epinephelus marginatus (Teleostei: Serranidae), induced by a non-steroidal aromatase inhibitoren
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy

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