Protective effect of beta-glucan extracted from Saccharomyces cerevisiae, against DNA damage and cytotoxicity in wild-type (k1) and repair-deficient (xrs5) CHO cells

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dc.contributor.authorOliveira, Rodrigo Juliano
dc.contributor.authorMatuo, Renata
dc.contributor.authorda Silva, Ariane Fernanda
dc.contributor.authorMatiazi, Hevenilton Jose
dc.contributor.authorMantovani, Mario Sergio
dc.contributor.authorRibeiro, Lucia Regina
dc.contributor.institutionUniversidade Estadual de Londrina (UEL)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T13:54:22Z
dc.date.available2014-05-20T13:54:22Z
dc.date.issued2007-02-01
dc.description.abstractA large number of functional foods, including those that contain P-glucan, have been shown to prevent the development of cancer and other chronic diseases. The aim of the present study was to elucidate its mechanism of action, as well as to understand its effects as an antigenotoxic, anticlastogenic agent, and to determine its capacity to preserve cell viability. The investigation was carried out in the CHO-k1 and CHO-xrs5 cell lines. The cytokinesis-blocked micronucleus assay indicated that the different doses of beta-glucan examined (5, 10, 20 and 40 mu g/ml) did not show clastogenic effects. In the CHO-k1 cell line, a chemopreventive effect could be observed in all the protocols tested: pre-treatment (% reduction of 35.0-57.3), simultaneous treatment (simple - 5 reduction of 19.7-55.6 and with pre-incubation - of 42.7-56.4) and post-treatment (% reduction of 17.9-37.6). This finding indicates mechanisms of action involving desmutagenesis and bio-antimutagenesis, albeit the latter having a lesser role. However, in the repair-deficient CHO-xrs5 cells, beta-glucan did not show a protective effect with post-treatment (% reduction of 2.96), thus supporting the involvement of bioantimutagenesis. The comet assay in CHO-k1 cells demonstrated that beta-glucan has neither a genotoxic nor an antigenotoxic effect. Cell viability tests indicated that beta-glucan preserves cell viability in both cell lines, preventing apoptotic events. These findings suggest that beta-glucan, when present in foods, could provide them with nutraceutical characteristics and act as a dietary supplement, or that P-glucan could be used in new drug development. (c) 2006 Elsevier Ltd. All rights reserved.en
dc.description.affiliationUniv Estadual Londrina, Dept Biol Geral, Londrina, PR, Brazil
dc.description.affiliationUniv Estadual Londrina, Dept Tecnol Alimentos & Medicamentos, Londrina, PR, Brazil
dc.description.affiliationUniv Estadual Paulista, Dept Biol Celular, Rio Claro, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Dept Biol Celular, Rio Claro, SP, Brazil
dc.format.extent41-52
dc.identifierhttp://dx.doi.org/10.1016/j.tiv.2006.07.018
dc.identifier.citationToxicology In Vitro. Oxford: Pergamon-Elsevier B.V., v. 21, n. 1, p. 41-52, 2007.
dc.identifier.doi10.1016/j.tiv.2006.07.018
dc.identifier.issn0887-2333
dc.identifier.urihttp://hdl.handle.net/11449/19433
dc.identifier.wosWOS:000244169500006
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofToxicology in Vitro
dc.relation.ispartofjcr3.105
dc.relation.ispartofsjr0,931
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectbeta-glucanpt
dc.subjectCHO-xrs5 cellspt
dc.subjectCHO-k1pt
dc.subjectcomet assaypt
dc.subjectcytokinesis-blocked micronucleus testpt
dc.titleProtective effect of beta-glucan extracted from Saccharomyces cerevisiae, against DNA damage and cytotoxicity in wild-type (k1) and repair-deficient (xrs5) CHO cellsen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Rio Claropt

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