Produção de β-glucanases por Trichoderma reesei e Trichoderma harzianum e aplicação na hidrólise de β-glucanas
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Data
2012-02-23
Autores
Carneiro, Andréia Aparecida Jacomassi [UNESP]
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Universidade Estadual Paulista (Unesp)
Resumo
As glucanas fúngicas têm sido muito estudadas por apresentarem respostas biológicas benéficas à saúde. O Agaricus blazei, conhecido popularmente como cogumelo do sol, é um basidiomiceto que apresenta propriedades funcionais devido às β-glucanas. Os fungos Trichoderma harzinaum e Trichoderma reesei foram capazes de se desenvolverem em Agaricus blazei em pó como única fonte de carbono produzindo β-1,3-glucanases, analisadas por superfície de resposta. As enzimas hidrolisaram β-glucanas de A. blazei e produziu glucose e diferentes glucooligossacarídeos. As enzimas brutas do T. harzianum e T. reesei hidrolisaram menos de 15 % de glucana e em torno de 40 % de laminarina (β-1,3 glucana comercial), após 60 minutos, respectivamente. Utilizando a β-1,3-glucanase bruta de T. harzianum foram detectados gentiobiose e laminaritriose nos hidrolisados enzimáticos de β- glucana e laminarina, enquanto a laminaritetraose, celotetraose e celotriose foram identifificados e quantificados apenas nos hidrolisados de β-glucana de A. blazei. A ação da β- 1,3-glucanase bruta de T. reesei sobre a laminarina e glucana de A. blazei resultou em glicose e gentiobiose. O que sugere uma possível diferença na ação catalítica das duas enzimas. As enzimas parcialmente purificadas do T. harzianum e T. reesei hidrolisaram 47 e 85 % de laminarina, respectivamente. A β-1,3-glucanase purificada de T. harzianum não degradou a glucana de A. blazei, e a enzima purificada de T. reesei degradou 2,6 % de glucana, após 60 minutos, no entanto, gentiobiose e laminaritriose foram detectados no hidrolisado de laminarina. Utilizando a enzima parcialmente purificada de T. reesei gentiobiose foi detectado no hidrolisado de glucana e de laminarina, e celotriose e laminaritriose foram detectados apenas no hidrolisado de laminarina. A fim de conhecer melhor...
Fungal glucans have been studied extensively because of their biological responses, which have been shown to possess health benefits. Agaricus blazei, commonly known as mushroom of the sun, is a basidiomycete that has functional properties because of its β-glucans. The fungi Trichoderma harzinaum and Trichoderma reesei were able to develop in a powdered form of A. blazei, which served as the only carbon source. The result was the production of β- 1,3-glucanases, which were analyzed using response surface methodology. The enzymes hydrolyzed the β-glucans of A. blazei, and produced glucose and different glucooligosaccharides. The crude enzymes of T. harzianum and T. reesei hydrolyzed less than 15% of glucan and approximately 40% of laminarin after 60 minutes, respectively. Using crude β-1,3-glucanase from T. harzianum, gentiobiose and laminaritriose were detected in enzymatic hydrolysates of β-glucan and laminarin, while laminaritetraose, cellotriose and celotetraose were identified and quantified only in the hydrolysates of the β-glucan of A. blazei. The action of the crude β-1,3-glucanase of T. reesei on the laminarin and glucan of A. blazei resulted in glucose and gentiobiose. These results suggest a possible difference in the catalytic action of the two enzymes. The partially purified enzymes of T. harzianum and T. reesei hydrolyzed 47% and 85% of laminarin, respectively. The purified β-1,3-glucanase from T. harzianum did not degrade the glucan of A. blazei, and the purified enzyme from T. reesei degraded 2.6% of the glucan after 60 minutes; however, gentiobiose and laminaritriose were detected in the hydrolyzates of laminarin. Using partially purified enzymes from T. reesei, gentiobiose was detected in the hydrolyzates of both glucan and laminarin, and laminaritriose and cellotriose and were detected... (Complete abstract click electronic access below)
Fungal glucans have been studied extensively because of their biological responses, which have been shown to possess health benefits. Agaricus blazei, commonly known as mushroom of the sun, is a basidiomycete that has functional properties because of its β-glucans. The fungi Trichoderma harzinaum and Trichoderma reesei were able to develop in a powdered form of A. blazei, which served as the only carbon source. The result was the production of β- 1,3-glucanases, which were analyzed using response surface methodology. The enzymes hydrolyzed the β-glucans of A. blazei, and produced glucose and different glucooligosaccharides. The crude enzymes of T. harzianum and T. reesei hydrolyzed less than 15% of glucan and approximately 40% of laminarin after 60 minutes, respectively. Using crude β-1,3-glucanase from T. harzianum, gentiobiose and laminaritriose were detected in enzymatic hydrolysates of β-glucan and laminarin, while laminaritetraose, cellotriose and celotetraose were identified and quantified only in the hydrolysates of the β-glucan of A. blazei. The action of the crude β-1,3-glucanase of T. reesei on the laminarin and glucan of A. blazei resulted in glucose and gentiobiose. These results suggest a possible difference in the catalytic action of the two enzymes. The partially purified enzymes of T. harzianum and T. reesei hydrolyzed 47% and 85% of laminarin, respectively. The purified β-1,3-glucanase from T. harzianum did not degrade the glucan of A. blazei, and the purified enzyme from T. reesei degraded 2.6% of the glucan after 60 minutes; however, gentiobiose and laminaritriose were detected in the hydrolyzates of laminarin. Using partially purified enzymes from T. reesei, gentiobiose was detected in the hydrolyzates of both glucan and laminarin, and laminaritriose and cellotriose and were detected... (Complete abstract click electronic access below)
Descrição
Palavras-chave
Enzimas, Glucanas, Polimerização, Agaricus blazei, Enzymes
Como citar
CARNEIRO, Andréia Aparecida Jacomassi. Produção de β-glucanases por Trichoderma reesei e Trichoderma harzianum e aplicação na hidrólise de β-glucanas. 2012. 186 f. Tese - (doutorado) - Universidade Estadual Paulista, Instituto de Biociências de Rio Claro, 2012.