Eficiência reprodutiva de camundongos endogâmicos balb/c em diferentes idades de acasalamento, com ou sem enriquecimento ambiental
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Data
2011-07-27
Autores
Moreira, Virgínia Barreto [UNESP]
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Editor
Universidade Estadual Paulista (Unesp)
Resumo
No Brasil, os biotérios de produção precisam atender elevada demanda de animais, com padrões genéticos e sanitários definidos e, por outro lado, existe a necessidade de considerar a crescente preocupação de setores da sociedade e dos órgãos reguladores, que trazem à discussão o tema do bem-estar dos animais de laboratório, em condição de cativeiro, mesmo que não estejam submetidos à experimentação. O objetivo deste estudo foi avaliar três idades de acasalamento (28, 45 e 60 dias) para camundongos da linhagem BALB/c, alojados em gaiolas enriquecidas ou não e suas influencias no desempenho produtivo. O experimento teve a duração de 310 dias, do primeiro acasalamento até o desmame da última ninhada. Usou-se 60 casais de camundongos da linhagem BALB/c, de padrão sanitário controlado, criados e mantidos em ambiente padronizado. Eles foram distribuídos em um delineamento inteiramente casualizado com arranjo fatorial 3 x 2 (três idades de acasalamento e alojamento com ou sem enriquecimento ambiental). Como forma de enriquecimento da gaiola foram utilizados tubos de papelão (com aproximadamente 10 cm de comprimento e 4 cm de diâmetro), previamente embalados e autoclavados. Foram analisadas características relacionadas à fertilidade, idade ao primeiro parto, intervalo entre partos, número de nascidos e desmamados totais e médio por ninhada, mortalidade pré-desmame e peso da ninhada ao desmame. Não houve efeito da interação enriquecimento x idade de acasalamento sobre as características estudadas. Tanto o enriquecimento, quanto a idade de acasalamento, influenciaram a mortalidade pré-desmame. Nas gaiolas enriquecidas a mortalidade pré-desmame foi mais baixa (P=0,01) que nas não enriquecidas (0,80 e 1,87 respectivamente). O acasalamento aos 28 dias resultou em mortalidade mais baixa (P = 0,01) comparado ao acasalamento aos 60 dias, enquanto o acasalamento...
In Brazil, the centers for production of laboratory animals need to meet a high demand for rodents with defined genetic and health status and, on the other hand, there is the need to consider the growing concern of the society and of the regulatory agencies regarding captive laboratory animals welfare, even though they are not under research protocols in those centers. The objective of this study was to evaluate the effect of three breeding ages (28, 45 and 60 days) on mice from the BALB/c strain, housed in enriched or non-enriched cages and their influence on the productive performance. The experiment lasted 310 days, from the first mating to weaning of the last litter. Sixty mouse couples from the BALB/c strain, with controlled health status, raised and kept under standardized environment were used. They were assigned to a completely randomized design in a 3 x 2 factorial arrangement (three breeding ages and cage with or without enrichment). Card paper tubes, approximately 10 cm long and with a diameter of 4 cm, were previously packed and autoclaved and used as cage enrichments. Traits related to fertility, age at first parturition, parturition interval, number born and weaned (total and litter average), pre-weaning mortality rate and litter weight at weaning were analyzed along the entire reproduction period. The breeding age x cage enrichment interaction did not affect any of traits under study, but each of these effects separately affected pre-weaning mortality. In the enriched cages, pre-weaning mortality was lower (P=0.01) than in non-enriched cages (0.80 vs. 1.87 deaths, respectively). Breeding at 28 days resulted in lower mortality (P= 0.01) compared to breeding at 60 days, whereas at 45 days an intermediary value was found (0.60, 2.25 and 1.16 deaths, respectively). No other effects of the treatments were detected (P>0.05) on the traits under study... (Complete abstract click electronic access below)
In Brazil, the centers for production of laboratory animals need to meet a high demand for rodents with defined genetic and health status and, on the other hand, there is the need to consider the growing concern of the society and of the regulatory agencies regarding captive laboratory animals welfare, even though they are not under research protocols in those centers. The objective of this study was to evaluate the effect of three breeding ages (28, 45 and 60 days) on mice from the BALB/c strain, housed in enriched or non-enriched cages and their influence on the productive performance. The experiment lasted 310 days, from the first mating to weaning of the last litter. Sixty mouse couples from the BALB/c strain, with controlled health status, raised and kept under standardized environment were used. They were assigned to a completely randomized design in a 3 x 2 factorial arrangement (three breeding ages and cage with or without enrichment). Card paper tubes, approximately 10 cm long and with a diameter of 4 cm, were previously packed and autoclaved and used as cage enrichments. Traits related to fertility, age at first parturition, parturition interval, number born and weaned (total and litter average), pre-weaning mortality rate and litter weight at weaning were analyzed along the entire reproduction period. The breeding age x cage enrichment interaction did not affect any of traits under study, but each of these effects separately affected pre-weaning mortality. In the enriched cages, pre-weaning mortality was lower (P=0.01) than in non-enriched cages (0.80 vs. 1.87 deaths, respectively). Breeding at 28 days resulted in lower mortality (P= 0.01) compared to breeding at 60 days, whereas at 45 days an intermediary value was found (0.60, 2.25 and 1.16 deaths, respectively). No other effects of the treatments were detected (P>0.05) on the traits under study... (Complete abstract click electronic access below)
Descrição
Palavras-chave
Camundongo, Produção animal, Bem-estar animal, Welfare, Environmental enrichment, Animal production
Como citar
MOREIRA, Virgínia Barreto. Eficiência reprodutiva de camundongos endogâmicos balb/c em diferentes idades de acasalamento, com ou sem enriquecimento ambiental. 2011. iii, 42 f. : grafs.,tabs. Dissertação (mestrado) - Universidade Estadual Paulista, Faculdade de Medicina Veterinária e Zootecnia de Botucatu, 2011.