Artigos - Fonoaudiologia - FFC

URI Permanente para esta coleção

https://hdl.handle.net/11449/10695

Navegar

Agora exibindo 1 - 20 de 791

Reading comprehension performance of elementary and senior high school students
(2023-01-01) de Oliveira, Adriana Marques [UNESP]; Santos, Jair Lício Ferreira; Capellini, Simone Aparecida [UNESP]; Federal University of Santa Maria; Universidade Estadual Paulista (UNESP); Universidade de São Paulo (USP)
Introduction: In Brazil, reading has been widely discussed, mainly due to the published results of national and international performance exams of Brazilian schoolchildren. Learning to read is therefore a continuous process, and the ability to make inferences while reading a text develops with age. The textual complexity involving the syntactic structuring, vocabulary and types of text progressively increases from the initial years of elementary school to high school students, also increasing the cognitive demand of the students; this occurs in parallel to their development and school advancement, which allows improvements in their teaching/learning processes. Based on the above, the following questions were raised: (1) How is the semantic process of reading established among elementary school students in elementary school (cycle II) and high school? Aim: to characterize the performance of elementary and senior high school students on semantic process tests from the Brazilian adaptation of the evaluation of reading processes (PROLEC-SE-R). Methods: A total of 436 students of both sexes, aged between 11 and 18 years, participated. They were evaluated with Assessment of Reading Processes-PROLEC-SE-R. Results and discussion: The results indicated that the semantic process was equally established among high school students, with a higher average performance compared with that of elementary school students. Among elementary school students, there was progression in the average correct answers with advancement in schooling. In the two levels of education, narrative texts allowed a greater number of correct answers, followed by multiple-choice and literal questions. The PROLEC-SE-R semantic process tests proved to be effective for assessing reading comprehension in elementary and high school students and reflected the Brazilian reality with regard to the gaps and weaknesses in the educational system.
Cytogenotoxic evaluations of leaves and stems extracts of Rubus rosifolius in primary metabolically noncompetent cells
(2023-01-01) de Quadros, Ana Paula Oliveira [UNESP]; Baraldi, Isabel Bragança [UNESP]; Petreanu, Marcel; Niero, Rivaldo; Mantovani, Mario Sergio; De Mascarenhas Gaivão, Isabel O’Neill; Maistro, Edson Luis [UNESP]; Universidade Estadual Paulista (UNESP); Vale do Itajaí University (UNIVALI); Universidade Estadual de Londrina (UEL); University of Trás-os-Montes and Alto Douro
Plants with medicinal potential may also produce adverse effects in humans. This seems to be the case for the species Rubus rosifolius, where preliminary studies demonstrated genotoxic effects attributed to extracts obtained from leaves and stems of this plant using on HepG2/C3A human hepatoma cells as a model. Considering the beneficial properties of this plant as an antidiarrheal, analgesic, antimicrobial, and antihypertensive and its effects in the treatment of gastrointestinal diseases, the present study was developed with the aim of determining the cytotoxic and genotoxic potential of extracts of leaves and stems of R. rosifolius in primary without metabolic competence in human peripheral blood mononuclear cells (PBMC). Cell viability analyses at concentrations of between 0.01 and 100 µg/ml of both extracts did not markedly affect cell viability. In contrast, assessment of the genotoxic potential using the comet assay demonstrated significant damage to DNA within PBMC from a concentration of 10 µg/ml in the stem extract, and a clastogenic/aneugenic response without cytokinesis-block proliferation index (CBPI) alterations at concentrations of 10, 20, or 100 µg/ml for both extracts. Under our experimental conditions, the data obtained demonstrated genotoxic and mutagenic effects attributed to extracts from leaves and stems of R. rosifolius in cells in the absence of hepatic metabolism.
Effects of a Competitive Season on Autonomic Heart Rate Modulation in Field Soccer Athletes
(2019-01-01) Miranda, JOÃO MARCELO DE QUEIROZ; Maia, Adriano Fortes; Rica, Roberta Luksevicius; Barbosa, Welmo Alcantara; Souza, ALANA DE SENA; Bergamin, Marco; Valenti, Vitor Engracia [UNESP]; Baker, Julien Steven; Shimojo, Guilherme Lemos; Prestes, Jonato; Stone, Whitley J.; Bocalini, Danilo S.; Wichi, Rogério Brandão; Universidade de São Paulo (USP); Federal University of Espírito Santo (UFES); Estacio de Sá University; University of Padova (UP); Universidade Estadual Paulista (UNESP); University of the West of Scotland; Catholic University of Brasilia; Western Kentucky University; Universidade Federal de Sergipe (UFS)
The physical demands of soccer combined with the rigor of the competitive season may have a substantial impact on autonomic modulation in field soccer athletes. The number of sudden death cases associated with soccer may be related to the physical training required to maintain performance and fitness, minimal time for recovery, and recurrent game participation. It is possible to identify individuals at risk of cardiovascular events by measuring heart rate variability (HRV), which is an indirect method for assessing autonomic activity. Therefore, the objective of this study was to analyze HRV before and after a period of field soccer competition. We evaluated 17 healthy male professional field soccer athletes and 12 untrained controls. The HRV was analyzed during supine rest before and after a period of field soccer competition. The following parameters were evaluated: interval R wave variation (RR), standard deviation of normal–normal intervals (SDNN), Root-mean-square successive difference (RMSSD), low frequency component (LF), high-frequency component (HF) and sympathovagal balance (LF/HF). Results indicated that the RR (p < .05, ES: 2.77), SDNN (p < .05, ES: 1.70), LF (p < .05, ES: 1.86), HF (p <.05, ES: 0.89) and LF/HF (p < .05, ES: 0.89) all decreased after the competition in the professional athletes with no change observed in the control group. In conclusion, the data suggest that a soccer competition negatively influences the autonomic regulation of heart rate.
Translation and cultural adaptation of the HELPS Reading Fluency Program into Brazilian Portuguese: A report of systematic adaptation processes and initial evidence of efficacy
(2023-02-09) Martins, Maíra Anelli [UNESP]; Begeny, John C.; Capellini, Simone Aparecida [UNESP]; Universidade Estadual Paulista (UNESP); North Carolina State University
Introduction: Across multiples languages, research demonstrates the important relationship between reading fluency and comprehension. Put simply, a fluent reader has greater attention and memory resources to use higher-order functions in reading, resulting in better comprehension of text. Some reading fluency interventions have shown positive results in improving students’ text reading fluency and comprehension; however, this research has predominantly been conducted with English-speaking students. For instance, until this report, a comprehensive search revealed only one prior study that evaluated an intervention strategy designed to improve students’ reading fluency in Brazilian Portuguese and no prior studies evaluated an intervention program with that population of students. Methods: The main goals of this two-part project were to (a) systematically translate, culturally adapt, and pilot test the Helping Early Literacy with Practice Strategies (HELPS) reading fluency program for use in Brazilian Portuguese (referred to as, HELPS-PB); and (b) conduct a preliminary quasi-experimental study of the HELPS-PB program with 23 students in grades 3 to 5 who needed a reading fluency intervention. Results and Discussion: This report documents the processes and successful adaptation of existing English- and Spanish-versions of HELPS into a new HELPS-PB program. It also offers preliminary evidence showing that students receiving HELPS-PB significantly improved their text reading fluency comparted to students in a control group. Implications for research, practice, and the adaptation of reading fluency programs into other languages are discussed.
Is Musical Auditory Stimulation Able to Influence the Recovery of Autonomic Modulation After Exercise?
(2023-03-01) Gomes, Rayana Loch [UNESP]; Ribeiro, Felipe [UNESP]; Valenti, Vítor Engrácia [UNESP]; Da Silva, Anne K. França [UNESP]; Gonzaga, Luana Almeida [UNESP]; Pérego, Sany Martins [UNESP]; de Marco, Rafael Luiz [UNESP]; Vanderlei, Luiz C. Marques [UNESP]; Universidade Estadual Paulista (UNESP)
Context • The effects of musical auditory stimulation on the human body have received considerable interest, especially when related to health and wellbeing, so the objective was to verify the influence of classical music exposure on autonomic modulation in recovery after exercise. Methods • 35 healthy young men aged 21.74 ± 2.59 years were evaluated. Volunteers perform 2-day evaluations, 1 day without musical stimulus and 1 with musical stimulation through classical music during exercise and recovery. Physical exercises were performed on a treadmill with an intensity of 6.0 km/hr + 1% of inclination in the first 5 minutes for physical “warm-up”, followed by 25 minutes with an intensity equivalent to 60% of the maximum velocity (Vmax), with a same slope according to the Conconi’s threshold. Finally, the volunteers remain at rest for 60 minutes for recovery. Heart rate variability (HRV) was analyzed in some moments of recovery by linear (RMSSD, SDNN, LF and HF (milliseconds squared and normalized unit) and LF/HF ratio, RRtri, TINN and the Poincaré plot: SD1, SD2 and SD1/SD2) and nonlinear indexes (DFA Total, DFA Alpha 1 and Symbolic Analysis: Shannon’s Entropy, 0V%, 1V%, 2LV% and 2ULV%). Results • No differences were found in the comparison between the values of the HRV indexes between protocols and interaction moments vs protocols. Differences were observed between the moments for most indexes (P < .05). Conclusions • Classical music did not significantly influence the autonomic control of heart rate in the recovery phase after submaximal exercise.
Handwriting fluency, latency, and kinematic in Portuguese writing system: Pilot study with school children from 3rd to 5th grade
(2023-01-12) Germano, Giseli Donadon [UNESP]; Capellini, Simone Aparecida [UNESP]; Universidade Estadual Paulista (UNESP)
Studies have referred to the interaction between orthographic and motor aspects during the production of handwriting. However, studies with Brazilian Portuguese are still lacking. Hence, the aim of this study was to compare orthographic regularity, based on the Portuguese writing system, in high (HF) and low (LF) frequency words, in relation to latency and kinematic variables in students from the 3rd to the 5th grade of elementary school. This is a cross-sectional pilot study, with a convenience sample of 95 children participated in this study, from 3rd to 5th grade level attending a state elementary school. All were submitted to the following procedures of computerized evaluation of handwriting and submitted to the task of writing 15 HF and 15 LF words, selected according to the frequency criteria and classified according writing coding rule. Results indicated that for HF words, there was a decrease in writing and disfluencies production time, for all coding rules, from 3rd to 5th grade. However, for LF words, the more unpredictable orthographic affect production duration time, movement fluency, and students became more dependent on the use of gaze to check spelling aspects. This study revealed that lexical and sub-lexical activation affected motor production. For HF and LF words, lexical and sublexical process favored motor programming. However, for LF words, despite the maturation and school progression for the motor planes, there was an increase in latency time and in the need to search for word information, measured by the gaze variable for words with greater irregularity. This study has provided some evidence that linguistic variables such as orthographic regularity and word familiarity affect handwriting performance in Brazilian Portuguese written language.
Use of secondary cues in prosodic focus marking in speech of children with phonological disorder
(2022-01-01) Soncin, Geovana [UNESP]; Polli, Luiza [UNESP]; Berti, Larissa Cristina [UNESP]; Universidade Estadual Paulista (UNESP)
This paper presents an acoustic description of prosodic focus marking in speech of children with phonological disorder to identify which phonetic cues can be seen as markers of contrastive focus. The data was obtained in speech evaluation sessions through a task of repeating focus marking sentences. Duration, intensity and intonation on focused words were the phonetic cues under analysis. Results show that prosodic focus marking in speech of children with phonological disorder is characterized by increased duration and intensity, but is not characterized by the use of contrastive focus marking nuclear accent. These results are discussed considering, on one hand, the combination of phonetic cues relevant for characterizing the production of contrastive focus and, on the other hand, its clinical implications. We present contributions for both linguistic and clinical studies in language acquisition
Obstructive sleep apnea and orofacial myofunctional aspects in obesity
(2022-01-01) da Silva, Nathani Cristina [UNESP]; da Silva, Giovanna Pietruci Junqueira Thomaz [UNESP]; Onofri, Suely Mayumi Motonaga [UNESP]; Pinato, Luciana [UNESP]; Universidade Estadual Paulista (UNESP)
Purpose: This study aimed to evaluate the quality of sleep, presence of obstructive sleep apnea (OSA), and its relationship with the presence of snoring, cephalometric characteristics, degree of collapse of the upper airways, and orofacial myofunctional profile in obese and overweight individuals. Methods: All participants completed polysomnography, as well as sleep and snoring questionnaires. We further performed orofacial, otorhinolaryngological, and anthropometric evaluations on all participants. Results: A total of 102 adults, comprising 29 obese, 21 overweight, and 52 eutrophic individuals of both sexes participated in this study. We observed a high prevalence of snoring in both obese and overweight (100%), and in 65% of eutrophic individuals. Among the obese subjects 58% had a severe degree of OSA, whereas 4% of eutrophic subjects presented a risk for OSA development. Sleep quality was related to body mass index (BMI) and cervical and abdominal circumference. All obese and overweight individuals presented with orofacial myofunctional alterations such as facial asymmetry, alteration of the maxilla-jaw relationship, inadequate tongue posture, changes in masticatory pattern and swallowing, and inadequate general orofacial myofunctional condition. Airway obstructions at the retropalatal and retrolingual levels > 75% were observed in at least 48% of the individuals. Conclusion: Obese and overweight individuals presented a higher risk for the development of OSA compared with eutrophic patients, and obese individuals presented a greater severity of OSA. The higher the BMI and greater the cervical and abdominal circumferences, the higher the prevalence of OSA, worse the quality of sleep, and more serious orofacial myofunctional characteristics in this population.
Efficacy of products for bleaching and whitening under orthodontic brackets
(2022-01-01) Pinzan-Vercelino, Célia Regina Maio; Lima, Suellen Nogueira Linares; Pereira, Fernando Félix de Jesus Vieira; Gurgel, Júlio de Araújo [UNESP]; da SILVA, Gisele Rodrigues; de FREITAS, Karina Maria Salvatore; Centro Universitário Uningá; Universidade Ceuma; Universidade Estadual Paulista (UNESP); Universidade Federal de Uberlândia (UFU)
Introduction: Many patients wearing orthodontic appliances request alterations in the shade of their teeth during orthodontic treatment. Objective: This study aimed to evaluate the efficacy of different products for bleaching and whitening under orthodontic brackets. Methods: Seventy bovine incisors were randomly divided into five groups (n = 14): C) non-whitening toothpaste (control); WTsi) hydrated silica whitening tooth-paste; WThp) 2% hydrogen peroxide whitening toothpaste; OB) in-office bleaching; and HB) at-home bleaching. Two buccal surface areas were evaluated using the Easyshade spec-trophotometer: under the metal bracket (experimental) and around the bracket (control). The paired t-test, ANOVA, and Tukey tests were applied for statistical analysis. Results: In-tragroup comparisons showed that in groups C, WThp and HB, there were statistically significant differences in the enamel color changes (ΔEab) between under and around the bracket areas (C-under bracket = 7.97 ± 2.35, around bracket = 2.86 ± 0.81, p < 0.01; WThp-under bracket = 4.69 ± 2.98, around bracket = 2.05 ± 1.41, p < 0.01; HB-under bracket = 7.41 ± 2.89, around bracket: 9.86 ± 3.32, p = 0.02). Groups WTsi, OB and HB presented similar perception of tooth whiteness (ΔWID) between the tested areas. Intergroup comparisons demonstrated that under the bracket area, the color change (ΔEab) was similar for all groups, except WThp (C = 7.97 ± 2.35; WTsi = 8.54 ± 3.63; WThp = 4.69 ± 2.98; OB = 9.31 ± 4.32; HB = 7.41 ± 2.89; p < 0.01). Conclusions: The dental color changes were effective for the products tested in groups WTsi, OB and HB in the presence of metallic orthodontic brackets.
Rubus rosifolius (Rosaceae) stem extract induces cell injury and apoptosis in human hepatoma cell line
(2023-02-01) De Quadros, Ana Paula Oliveira [UNESP]; Oshiiwa, Bruna [UNESP]; Petreanu, Marcel; Niero, Rivaldo; Rosa, Paulo Cesar Pires; Sawaya, Alexandra Christine Helena Frankland; Mantovani, Mario Sergio; O'Neill De Mascarenhas Gaivão, Isabel; Maistro, Edson Luis [UNESP]; Universidade Estadual Paulista (UNESP); Vale do Itajaí University (UNIVALI); Universidade Estadual de Campinas (UNICAMP); Universidade Estadual de Londrina (UEL); CECAV and Department of Genetics and Biotechnology
Rubus rosifolius, popularly known as “red mulberry”, is a common medicinal plant in southern Brazil and is used as an antidiarrheal, analgesic, antimicrobial and antihypertensive, and to treat stomach diseases. The aim of this study was to analyze the R. rosifolius stem extract (RrSE) for possible in vitro cytotoxic and genotoxic effects, using the comet assay and the micronucleus test to assess genotoxicity, and flow cytometry to assess the impact on the cell cycle and apoptosis in HepG2/C3A cells, in addition to evaluating the expression of genes linked to the induction of DNA damage, cell cycle, apoptosis and metabolism of xenobiotics. The MTT assay observed no cytotoxic effects at concentrations between 0.01 and 100 μg/mL of the extract. However, genotoxic effects occurred in treatments with the extract from a 1 μg/mL concentration. Flow cytometry analysis revealed a significant increase in cells in the G2/M phase after treatment with 10 μg/mL, a decrease in cells in the G0/G1 phase in the treatment with 100 μg/mL, and a significant increase in total apoptotic cells. In the gene expression analysis, an increase in the CYP1A2 xenobiotics metabolizing gene expression was observed. Despite the promising pharmacological effects of R. rosifolius, the results revealed that the RrSE has genotoxic effect and induces apoptosis in HepG2/C3A cells, indicating danger in using this plant extract by humans.
Speech Production Measures in Brazilian Portuguese Children With and Without Speech Sound Disorder
(2020-01-01) de Oliveira, Aline Mara; Veschi, Gabriely Vitória; Polli, Luiza [UNESP]; Esperandino, Cássio Eduardo [UNESP]; Berti, Larissa Cristina [UNESP]; Universidade Estadual Paulista (UNESP); Fernandópolis Educational Foundation – FEF
Risk of Dysphonia in Theater Actors: Proposal for a Screening Protocol
(2023-01-01) Ferrari, Eloisa Pinheiro; da Trindade Duarte, João Marcos; Simões-Zenari, Marcia; Vilela, Nadia; Master, Suely [UNESP]; Nemr, Katia; Universidade de São Paulo (USP); Escola de Atores Wolf Maya; Universidade Estadual Paulista (UNESP)
Objective: To propose a Dysphonia Risk Screening Protocol for Actors (DRSP-A), test its usability in conjunction with the General Dysphonia Risk Screening Protocol (G-DRSP), determine the cut-off point for a high risk of dysphonia in actors, and compare the risk of dysphonia between actors with and without voice disorders. Method: Observational cross-sectional study with 77 professional actors or students. The questionnaires were applied individually and the total scores were summed to calculate the final score of the Dysphonia Risk Screening (DRS-Final). The validity of the questionnaire was verified from the area under the Receiver Operating Characteristic (ROC) curve, and cut-offs were obtained based on diagnostic criteria for screening procedures. Voice recordings were collected for auditory-perceptual analysis and subsequent division into groups with and without vocal alteration. Results: The sample showed a high risk of dysphonia. Higher scores in the G-DRSP and in the DRS-Final were found in the group that presented vocal alteration. The cut-off points established for the DRSP-A and DRS-Final were 0.623 and 0.789, respectively, with higher degrees of sensitivity than specificity. Thus, above these values, the risk of dysphonia is greater. Conclusion: A cut-off value was calculated for the DRSP-A. This instrument was proven to be viable and applicable. The group with vocal alteration had a higher score in the G-DRSP and DRS-Final, but there was no difference in the DRSP-A.
Access to rehabilitation after stroke in Brazil (AReA study): multicenter study protocol
(2022-10-01) de Oliveira Cacho, Roberta; Cabral Moro, Carla Heloisa; Bazan, Rodrigo [UNESP]; da Guarda, Suzete Nascimento Farias; Pinto, Elen Beatriz; dos Santos Andrade, Suellen Mary Marinho; Valler, Lenise; Almeida, Kelson James; Ribeiro, Tatiana Souza; de Moura Jucá, Renata Viana Brígido; Minelli, Cesar; Pimentel Piemonte, Maria Elisa; Albuquerque Paschoal, Eric Homero; Araújo Pedatella, Marco Túlio; Pontes-Neto, Octávio Marques; Fontana, Ana Paula; de Souza Pagnussat, Aline; Conforto, Adriana Bastos; Faculdade de Ciências da Saúde do Trairi; Hospital Municipal de São José; Universidade Estadual Paulista (UNESP); Universidade Federal da Bahia (UFBA); Fundação para o Desenvolvimento das Ciências; Universidade Federal da Paraíba (UFPB); Universidade Estadual de Campinas (UNICAMP); Centro Universitário UniFacid; Universidade Federal do Rio Grande do Norte; Hospital Universitário Walter Cantídio; Instituto “Você sem AVC”; Universidade de São Paulo (USP); Hospital Ophir Loyola; Hospital Estadual de Urgência de Goiânia Dr Valdemiro Cruz; Universidade Federal do Rio de Janeiro (UFRJ); Universidade Federal de Ciências da Saúde de Porto Alegre; Hospital Israelita Albert Einstein
Background Most of the Brazilian population relies on public healthcare and stroke is a major cause of disability in this country of continental dimensions. There is limited information about access to rehabilitation after stroke in Brazil. Objective To provide comprehensive information about Access to Rehabilitation After discharge from public hospitals in Brazil (AReA study), up to 6 months after stroke. Methods The present study intends to collect information from 17 public health centers in 16 Brazilian cities in the 5 macroregions of the country. Each center will include 36 participants (n ¼ 612). The inclusion criteria are: age ≥ 18 years old; ischemic or hemorrhagic stroke, from 6 months to 1 year prior to the interview; admission to a public hospital in the acute phase after stroke; any neurological impairment poststroke; patient or caregiver able to provide informed consent and answer the survey. Patients can only be recruited in public neurology or internal medicine outpatient clinics. Outcomes will be assessed by a standard questionnaire about rehabilitation referrals, the rehabilitation program (current status, duration in months, number of sessions per week) and instructions received. In addition, patients will be asked about preferences for locations of rehabilitation (hospitals, clinics, or at home). Trial Status The study is ongoing. Recruitment started on January 31st, 2020 and is planned to continue until June 2022. Conclusion The AReA study will fill a gap in knowledge about access to stroke rehabilitation in the public health system in different Brazilian regions.
Satisfaction and Adherence of COPD Patients to a Conventional Training Associated with Functional Exercises and to a Conventional Training Isolated: A Qualiquantitative Study
(2022-01-01) Grigoletto, Isis [UNESP]; de Lima, Fabiano Francisco; Eto, Daniele Akemi [UNESP]; Suzuki, Natália Narumi Voltareli [UNESP]; Uzeloto, Juliana Souza [UNESP]; Ramos, Ercy Mara Cipulo [UNESP]; Universidade Estadual Paulista (UNESP); Universidade de São Paulo (USP)
Purpose: To verify, through quali-quantitative analysis, the satisfaction and adherence of patients with Chronic Obstructive Pulmonary Disease (COPD) to the insertion of functional circuit training into conventional training. Patients and Methods: 23 patients with COPD from a randomized clinical trial were invited to participate in a quali-quantitative analysis after the training finalization, divided into FTG (Functional Training Group) and CTG (Conventional Training Group). A total of 21 patients participated [(FTG: n=10; 65.80±7.31 years; FEV1/FVC: 56.44±12.67%) and CTG (n=11; 70.36±7.02 years; FEV1 /FVC: 55.89±8.20)]. For the qualitative evaluation, focus groups were performed, using a previously developed script. Adherence was verified by the presence in the training sessions that were prescribed, and the quantitative analysis was performed using questionnaires with multiple-choice questions (evaluation of the aspects that can interfere in a training). The participants were asked to define a grade between zero and ten regarding the aspects of the training (satisfaction). Results: In both groups, there was similar adherence (p=0.965) and satisfaction (p=0.341). The qualitative analysis identified seven themes and 17 codes, representing factors related to satisfaction and negative aspects, as follows: factors associated with satisfaction: self-efficacy management, physical and psychosocial improvement, interpersonal relationships, and proposed exercises. Negative aspects: pains, comorbidities, beliefs, and personal demotivation. In the quantitative analysis, was verified that an increase in the symptoms, the distance between home and training center, and personal problems were not factors that interfered in the adherence of the participants (p<0.05). Conclusion: Similar satisfaction and adherence of patients with COPD were observed in the FTG and CTG and patients from FTG reported higher fatigue.
Evaluation of in vitro cytotoxic and genotoxic effects of the 3-(3,4-dihydroxyphenyl)-8-hydroxycoumarin
(2023-01-01) dos Santos Silva, Edvannia; Matos, Maria João; Maistro, Edson Luis [UNESP]; FAMEMA; Universidade de Santiago de Compostela; Universidade Estadual Paulista (UNESP)
A wide variety of natural and synthetic coumarins present therapeutic potential. Therefore, the assessment of their safety for humans is essential. 3-(3,4-Dihydroxyphenyl)-8-hydroxycoumarin is a coumarin derivative with antioxidant properties, among other biological activities. The aim of this study is to evaluate the cytotoxic and genotoxic potential of this molecule on peripheral blood mononuclear cells (PBMC) and human hepatocellular carcinoma cells (HepG2/C3A). The results obtained for the cytotoxicity assays, evaluated by the trypan blue staining assay, using concentrations between 0.1 and 20 μg/mL, showed that there is no decrease in cell viability for both cell lines. The MTT assay showed a significant decrease in the viability of HepG2/C3A cells at the highest concentrations tested, after 48 h, for all the tested concentrations, after 72 h of exposure. Regarding the genotoxic assays, the data obtained by the comet assay and the micronucleus test, up to the tested concentration of 10 μg/mL, do not show significant DNA damage and/or chromosomal mutations, for both cell lines. However, at the highest tested concentration of 20 μg/mL, a small but significant genotoxic effect was observed in PBMC. In view of the observed results, it can be concluded that the 3-(3,4-dihydroxyphenyl)-8-hydroxycoumarin, up to a concentration of 10 μg/mL, does not present genotoxic effects in human cells with and without liver enzymes metabolism. Additional studies with higher concentrations of this molecule need to be performed to address its complete biosafety.
Fundamental frequency related parameters in Brazilians with COVID-19
(2023-01-01) Berti, Larissa Cristina [UNESP]; Spazzapan, Evelyn Alves [UNESP]; Queiroz, Marcelo; Pereira, Pedro Leyton; Fernandes-Svartman, Flaviane Romani; Medeiros, Beatriz Raposo De; Martins, Marcus Vinícius Moreira; Ferreira, Letícia Santiago; Silva, Ingrid Gandolfi Gomes Da; Sabino, Ester Cerdeira; Levin, Anna Sara; Finger, Marcelo; Universidade Estadual Paulista (UNESP); Universidade de São Paulo (USP)
This study compares fundamental frequency (fo) and fundamental frequency standard deviation (foSD) of COVID-19 patients with the same parameters in the speech of subjects without COVID-19, and verifies whether there is an effect of age and sex in the patient group. Both groups, subjects with and without COVID-19, are formed by Brazilian Portuguese speakers. Speech samples were obtained from 100 patients with mild to severe symptoms of COVID-19, and 100 healthy subjects. A single 31-syllable Portuguese sentence was used as the elicitation material for all subjects. The recordings were divided into four age groups. The acoustic measures were semi-Automatically extracted and analyzed by a series of analyses of variance. Patients with COVID-19 present vocal differences in fo-related parameters when compared to healthy subjects, that is, patient voices presented higher fo and foSD with respect to control voices. In addition, for patient voices, there was an age and sex effect on fo SD values. Vocal parameters of women and elderly subjects showed more marked differences in fo-related parameters, indicating that patient voices are higher-pitched and have a higher variation of fo SD. Consequently, fo-related parameters may be tested as vocal biomarkers in the screening of respiratory insufficiency by voice analysis, in patients with severe symptoms of COVID-19.
Tissue Bioengineering with Fibrin Scaffolds and Deproteinized Bone Matrix Associated or Not with the Transoperative Laser Photobiomodulation Protocol
(2023-01-01) Pomini, Karina Torres; Buchaim, Daniela Vieira; Bighetti, Ana Carolina Cestari; Hamzé, Abdul Latif; Reis, Carlos Henrique Bertoni; Duarte, Marco Antonio Húngaro; Alcalde, Murilo Priori; Barraviera, Benedito [UNESP]; Júnior, Rui Seabra Ferreira [UNESP]; de Souza, Alexandre Teixeira; da Silva Santos, Paulo Sérgio; Pilon, João Paulo Galletti [UNESP]; de Marchi, Miguel Ângelo; Nogueira, Dayane Maria Braz; de Souza Bueno, Cleuber Rodrigo; Soares, Wendel Cleber; Buchaim, Rogerio Leone; Universidade de São Paulo (USP); University of Marilia (UNIMAR); University Center of Adamantina (UNIFAI); Universidade Estadual Paulista (UNESP); UNINOVE University
Extending the range of use of the heterologous fibrin biopolymer, this pre-clinical study showed a new proportionality of its components directed to the formation of scaffold with a lower density of the resulting mesh to facilitate the infiltration of bone cells, and combined with therapy by laser photobiomodulation, in order to accelerate the repair process and decrease the morphofunctional recovery time. Thus, a transoperative protocol of laser photobiomodulation (L) was evaluated in critical bone defects filled with deproteinized bovine bone particles (P) associated with heterologous fibrin biopolymer (HF). The groups were: BCL (blood clot + laser); HF; HFL; PHF (P+HF); PHFL (P+HF+L). Microtomographically, bone volume (BV) at 14 days, was higher in the PHF and PHFL groups (10.45 ± 3.31 mm3 and 9.94 ± 1.51 mm3), significantly increasing in the BCL, HFL and PHFL groups. Histologically, in all experimental groups, the defects were not reestablished either in the external cortical bone or in the epidural, occurring only in partial bone repair. At 42 days, the bone area (BA) increased in all groups, being significantly higher in the laser-treated groups. The quantification of bone collagen fibers showed that the percentage of collagen fibers in the bone tissue was similar between the groups for each experimental period, but significantly higher at 42 days (35.71 ± 6.89%) compared to 14 days (18.94 ± 6.86%). It can be concluded that the results of the present study denote potential effects of laser radiation capable of inducing functional bone regeneration, through the synergistic combination of biomaterials and the new ratio of heterologous fibrin biopolymer components (1:1:1) was able to make the resulting fibrin mesh less dense and susceptible to cellular permeability. Thus, the best fibrinogen concentration should be evaluated to find the ideal heterologous fibrin scaffold.
O benefício do mascaramento modulado nos potenciais evocados auditivos corticais com estímulo de fala
(2022-01-01) Rocha, Mônyka Ferreira Borges; Menezes, Denise Costa; Duarte, Danielle Samara Bandeira; Griz, Silvana Maria Sobral; Frizzo, Ana Claudia Figueiredo [UNESP]; Menezes, Pedro de Lemos; Teixeira, Cleide Fernandes; Advíncula, Karina Paes; Universidade Federal de Pernambuco (UFPE); Universidade Estadual Paulista (UNESP); Universidade Estadual de Ciências da Saúde de Alagoas - UNCISAL - Maceió (AL)
PURPOSE: To analyze the effect of masking on the Cortical Auditory Evoked Potential with speech stimulus in young adults. METHODS: Fourteen individuals aged between 19 and 28 years of both sexes with no hearing loss participated in the study. The Cortical Auditory Evoked Potential examination was performed with synthetic speech stimulus /ba/ simultaneous to Speech Shaped Noise presented under three conditions: steady noise with a 30 dB SPLep intensity (weak steady noise), steady noise with a 65 dB SPLep intensity o (strong steady noise) and modulated noise with 30 dB SPLep and 65 dB SPLep intensities at 25Hz and modulation period of 40 ms. RESULTS: Higher latencies were observed in the cortical components, except P2, in the condition of strong steady noise and more meaningful measures of amplitude of the cortical components P1, N1 and P2 in the condition of modulated noise with statistically significant difference in comparison to the strong steady noise condition. There was worse wave morphology in the condition of strong steady noise, when compared to the other records. The average electrophysiological thresholds for the conditions of strong steady noise and modulated noise were 60 dB SPLep and 49 dB SPLep, respectively, showing a 11.7 dB mean difference. CONCLUSION: We could infer that there was a lower masking effect of modulated noise when compared to the strong steady noise condition, in the amplitude measurements of the cortical components and an average difference of 11.7 dB between the electrophysiological thresholds (interpreted as the measure of the Masking Release).
ORTHOGRAPHIC PERFORMANCE PROFILE OF STUDENTS WITH DYSLEXIA AND LEARNING DIFFICULTIES
(Univ Estadual Paulista-unesp, Fac Ciencias Letras Assis, 2022-01-01) Chiaramonte, Thais Contiero [UNESP]; Capelini, Simone Aparecida [UNESP]; Universidade Estadual Paulista (UNESP)
It is described in the literature that students with specific learning difficulties and disorders may present difficulties in the spelling appropriation of the Brazilian Portuguese writing system, however little is discussed about which errors are most committed by these populations. Thus, this study aimed to characterize and compare the orthographic performance of students with Dyslexia, Learning Disabilities and good academic performance. 75 school children participated, from 9 to 11 years old, divided into 3 groups and matched by diagnosis and school year. Spelling was evaluated by dictating words and pseudowords based on the semiology of errors. The results were compared statistically by the Kruskal-Wallis test adjusted by the Bonferroni Correction, where it was possible to observe that the spelling profile of students with dyslexia and learning difficulties are similar, while the profile of students with good academic performance differs from the other groups, also concluding that the Pseudowords Dictation test is the best to differentiate students with Dyslexia of students with learning difficulties and good academic performance.
Speech Production Measures in Brazilian Portuguese Children With and Without Speech Sound Disorder
(Multilingual Matters Ltd, 2020-01-01) Oliveira, Aline Mara de; Veschi, Gabriely Vitoria; Polli, Luiza [UNESP]; Esperandino, Cassio Eduardo [UNESP]; Berti, Larissa Cristina [UNESP]; Babatsouli, E.; Universidade Federal de Santa Catarina (UFSC); Fernandopolis Educ Fdn FEF; Universidade Estadual Paulista (UNESP)

Navegar

Submissões Recentes