Trabalhos de Conclusão de Curso - IBB

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Simplificação dos resultados no teste respiratório com aminopirina marcada com isótopo estável do 13C para avaliação da cirrose hepática
(Universidade Estadual Paulista (Unesp), 2016) Camargo, Kayque Roberto Fracarolli de [UNESP]; Costa, Vladimir Eliodoro [UNESP]; Universidade Estadual Paulista (Unesp)
For evaluation of respiratory tests it was used the delta over baseline-value (DOB), which is expressed by subtracting the sample isotopic enrichment (δ¹³C ‰ sample) at baseline isotopic enrichment (δ¹³C‰ basal). Another evaluation method was the percentage of recovered dose (PDR), which is to correct the test for each patient, taking into consideration the weight, the height, the amount of drug administered, DOB, and the production of CO2. It was observed that the methods of DOB and PDR are resemble, so the DOB can be used to evaluate the aminopyrine breath test marked on carbon 13 (¹³C-ABT). The aim of this study is to demonstrate that in breath tests with carbon 13, in which the dose of the drug is not unique, you can use the isotopic ratio DOB without the need to apply the PDR. It was used for this study ABT for liver evaluation of patients with cirrhosis due to HCV. The drug used for the test was purchased powder and administered orally by the patient in the amount of 2 mg / kg diluted in 200ml of water. We studied 16 patients, from Viral Hepatitis Clinic of Botucatu, divided into 2 groups, control and cirrhotic child A. For the test was performed to collect baseline breath, and after drug intake collection blow was performed every 2,5 minutes to half hour, every 5 minutes to 1 hour, and every 10 minutes up to 2 hours, a total of 2 hours. For the analysis of blowing was used the ABCA-IRMS (Automated Breath Carbon Analyzer- Isotope-ratio mass spectrometry). It was used those graphs ¹³C turnover curve PDR and DOB to compare the results. There was no significant difference in the two different methods of assessment may conclude as soon as the DOB is reliable for assessment of liver cirrhosis test
Sistema de integração lavoura - pecuária - floresta (ILPF): panorama nacional
(Universidade Estadual Paulista (Unesp), 2016) Sartori, André [UNESP]; Nagy, Silvio Carlos Santos [UNESP]; Universidade Estadual Paulista (Unesp)
Among several forms of environment's impact, it has been arising the degradation from introduction of pastures, the impoverishment of the soil, and suppression of vegetation deriving from crops. In this context, it becomes necessary the searching of new agricultural systems that lead to production and they need to be economic and sustainable according to the environmental conservation policies. Based on the environmental problems and the need for improvement in production efficiency, this work compiles information and data already published about the issue Interaction Crop, Livestock and Forest. Thus, we provide information for analysis and implementation of this new Integrated Production System
Influência do jejum seguido de realimentação no anabolismo e catabolismo no músculo vermelho do pacu (piaractus mesopotamicus)
(Universidade Estadual Paulista (Unesp), 2016) Zanella, Bruna Tereza Thomazini [UNESP]; Dal-Pai, Maeli [UNESP]; Paula, Tassiana Gutierrez de [UNESP]; Universidade Estadual Paulista (Unesp)
Not available
O papel da chuva de sementes na regeneração natural de corredores ecológicos
(Universidade Estadual Paulista (Unesp), 2016) Silva, Camila Rocha Pergentino da [UNESP]; Engel, Vera Lex [UNESP]; Universidade Estadual Paulista (Unesp)
Not available
Análise fisiológica de sementes de Handroanthus impetiginosus de quatro biomas brasileiro
(Universidade Estadual Paulista (Unesp), 2016) Santos, Camila Amaro dos [UNESP]; Silva, Edvaldo Aparecido Amaral da [UNESP]; Bravo, Juliana Pereira [UNESP]; Universidade Estadual Paulista (Unesp)
Brazil is a country with great biodiversity due to the wide range of soil, climate and vegetation, highlighting the Amazon Forest, Atlantic Forest, Caatinga and Cerrado. In view of this biodiversity, few studies have been done with native species. Among the native species present in the four biomes highlighted the Handroanthus impetiginosus, popularly known as ipe-purple, has great economic and ecological importance of highlighting the quality of the wood is used in folk medicine and currently being used in recovery programs of degraded areas the species under study has attracted the attention of researchers because of their ability to adapt to the different biomes. This study aimed to characterize seeds collected in four biomes on the sizing of seeds, protein quantitation, germination rate, water content and soaking speed. The analysis of scaling seeds, there was a great variation of the matrices in the observed results. The germination rate was assessed by counting the root protrusion correlating the results of water content. It was observed that lower water contents of the seeds are combined with higher values radicle protrusion. Analyzes with soaking speed were carried out and a similar pattern was observed between arrays from the same biome. The quantification of the protein showed that the seeds with higher protein contents are the same with greater speed of imbibition Keywords: handroanthus impetiginosus, seeds, soluble protein
Comparação da Avifauna em Dois Sistemas de Produção Agrícola, em Botucatu-SP
(Universidade Estadual Paulista (Unesp), 2016) Blanco, Beatriz Tenore [UNESP]; Fonseca, Renata Cristina Batista [UNESP]; Universidade Estadual Paulista (Unesp)
Not available
Influência do jejum seguido de realimentação na expressão de genes do anabolismo e catabolismo no músculo branco do pacu (Piaractus mesopotamicus)
(Universidade Estadual Paulista (Unesp), 2016) Oliveira, Caroline Bredariol de [UNESP]; Pai, Maeli Dal [UNESP]; Paula, Tassiana Gutierrez de [UNESP]; Universidade Estadual Paulista (Unesp)
Not available
Papel das metaloproteínases e das células satélites no músculo plantar durante o envelhecimento
(Universidade Estadual Paulista (Unesp), 2016) Mosele, Francielle Caroline [UNESP]; Pai, Maeli Dal [UNESP]; Universidade Estadual Paulista (Unesp)
Not available
Rotas de contaminação de formigas cortadeiras por contato direto e indireto com corante traçador lipossolúvel
(Universidade Estadual Paulista (Unesp), 2016) Puccini, Carolina de Lima [UNESP]; Forti, Luiz Carlos [UNESP]; Camargo, Roberto Da Silva [UNESP]; Universidade Estadual Paulista (Unesp)
Leaf-cutting ants are known to cut plants for cultivating the symbiotic fungus used as a food. For this reason, plantations are harmed by these insects. As a control, toxics baits are used. From this study we aim to demonstrate that direct contact behaviors (behaviors related to the processing of plant material) and indirect (self-grooming, allogrooming or touches) with toxic baits promote the contamination of workers. For the direct contact, observations were made and counted the frequencies of of worker behavior in the processing of pellets based on citrus pulp. We found that the behaviors of holding and licking the baits are the most recurrent. For indirect contact, topical application of tracer (dye, Sudan III) was performed on the worker, and social interactions were then studied between the worker with tracer and without tracer. Additionally, the workers' heads were dissected to visualize the presence of dye in the post-pharyngeal gland. The results were that from 50 to 70% of the workers had their pharyngeal gland stained, depending on the size of the group. The behavioral analysis showed an increasing interaction according to the increase of individuals, with the touches among the workers being the most frequent behavior. Probably the route of the tracer dye occurred by the excessive touching between the workers, with self-grooming and allogrooming. The direct and indirect contact behaviors analyzed are responsible for rapidly dispersing a substance among the members of the colony, as we have seen in our experiment. Thus, we corroborate the hypothesis that the direct and indirect contact promotes the contamination...
Potencial alelopático de rizomas de curcuma zedoaria (christm.) roscoe cultivados em condições de deficiência hídrica
(Universidade Estadual Paulista (Unesp), 2016) Vasconcellos, Gabriela Marin [UNESP]; Almeida, Luiz Fernando Rolim De [UNESP]; Universidade Estadual Paulista (Unesp)
Allelopathy is a phenomenon that promotes or inhibits the organisms growth and development as result of emission of allelochemicals (chemical compounds originating from the secondary metabolism) by allelopathic agents. Allelochemicals derivate from different organs in plants. Among the environment and agronomic conditions, water deficit affects secondary metabolites synthesis. Usually, there are an increase in the secondary metabolites concentration of plants under water deficit. The rhizome of Curcuma zedoaria (Christm.) Roscoe synthetizes an essential oil mainly constituted of terpene compounds. Therefore, the aim of this work is to evaluate the effects of water deficit upon the rhizome allelopathic potential of Curcuma zedoaria. Accordingly, seeds of sorghum (Sorghum bicolor L.) and cucumber (Cucumis sativus L.cv. Aodai) were exposed to aqueous extract of C. zedoaria rhizome. The C. zedoaria plants were cultivated in greenhouse into two groups apart: plants under controlled water supply (WS) or water deficit (WD). The aqueous extracts of rhizomes were extracted after sectioning and weighing into following concentrations: 5, 10, 50 and 100 g L-1. The biological assays were realized in the germination chamber (BOD) under controlled light and temperature conditions. We evaluated the final percentage of germination (G), germination speed index (GSI) and measurement of root and shoot growth. The rhizome aqueous extract of C. zedoaria inhibits the growth and development of seeds and seedling in mostly evaluated parameters in both treatments. Conversely, the cucumber seeds germination is not affected by the rhizome aqueous extract by neither of treatments, although, the seedling growth decreases in the higher concentration (100 g L-1) of WD group. Indeed, there is a modification in the secondary metabolites concentration/composition in the rhizome of C. zedoaria under water-deficit, mainly in the higher concentr
Efeitos da exposição subcrônica à sibutramina sobre parâmetros reprodutivos e fertilidade de ratos machos wistar adultos
(Universidade Estadual Paulista (Unesp), 2016) Missassi, Gabriela [UNESP]; Kempinas, Wilma De Grava [UNESP]; Universidade Estadual Paulista (Unesp)
Sibutramine is a non-selective serotonin-norepinephrine reuptake inhibitor largely used for weight loss. In a previous work, we showed the pharmacological mechanisms involved in the reduction on sperm quality and fertility of rats exposed to this anorexigen in the light phase of light-dark (l/d) cycle. Considering that rodents are nightlife animals, with higher metabolic activity during the dark phase, the present work aimed to further investigate whether the alterations found in rat sperm quality would be maintained after treatment in this period. For this, adult male Wistar rats were treated with sibutramine (10 mg/kg/day) or vehicle for 15 days in the dark phase of the light-dark cycle. Sibutramine decreased final body and reproductive organ weights, as well as the testosterone levels and number of sperm with progressive motility. These animals also had an acceleration in the sperm transit time in the epididymis and a decrease in sperm concentrations throughout this organ (histopathological analysis). In conclusion, the impairment of reproductive parameters were enhanced when the exposure occurred during the dark phase of the l/d cycle, which corresponds to more active period of these animals.
Avaliação da atividade anti-inflamatória intestinal do extrato hidroalcoólico de Solanum paniculatum L. (Solanaceae)
(Universidade Estadual Paulista (Unesp), 2016) Mori, Cristiane [UNESP]; Stasi, Luiz Claudio Di [UNESP]; Universidade Estadual Paulista (Unesp)
Inflammatory bowel disease is a term related to two distinct diseases, ulcerative colitis (UC) and Crohn's disease (CD), both diseases are characterized by chronic inflammation that compromise structurally and functionally the gastrointestinal tract. Its etiology is unknown, current treatments do not provide ultimate healing and brings numerous side effects, demonstrating the need of new therapeutic strategies, and the use of natural products is one of the most promising. Solanum paniculatum L., family Solanaceae, popularly known as jurubeba, is a plant with different medicinal uses and wide distribution in Brazil, popularly used in controlling arthritis (chronic inflammation), liver and gastrointestinal disorders. Hydroalcoholic extracts of S. paniculatum leaves were administered orally in four doses: 62.5 mg/kg, 125 mg/kg, 250 mg/kg and 500 mg/kg for 5 consecutive days. After this treatment, the induction of the inflammatory process was made by rectal administration of 0.25 ml solution of 10 mg TNBS acid in 50% ethanol (v/v). After 48 hours since induction of the inflammatory process, animals were killed and the colons were removed for macroscopic (lesion length and lesion score, colonic weight -length ratio, adherence to adjacent organs and diarrhea) and biochemical (total content of glutathione, myeloperoxidase and alkaline phosphatase enzyme activities) analyses. Results show that at the doses used the hydroalcoholic extract of Solanum paniculatum L. does not produced protective effect on the TNBS-induced colonic inflammation, suggesting no anti-inflammatory effect at the evaluated doses
Efeitos da eutrofização e da composição de espécies de macrófitas sob a emissão de gases de efeito estufa em lagos rasos
(Universidade Estadual Paulista (Unesp), 2016-07-04) Carlos, Anderson Ricardo [UNESP]; Kosten, Sarian [UNESP]; Universidade Estadual Paulista (Unesp)
Recent research indicates that eutrophication can concomitantly lead to higher greenhouse gas (GHG) emissions, in turn promoting climate change. Eutrophication tends to increase carbon dioxide (CO2) fixation in fresh water systems which might seem positive for global warming perspective. Nevertheless, eutrophication also promotes to increased absolute production and respiration, greater release of more nitrous oxide (N2O) and methane (CH4). Furthermore, the understanding about GHG flux pathways and the influence of macrophytes in the process still remain unclear. Therefore, this study intends to clarify the effects of eutrophication and macrophyte species on GHG specially from shallow lakes - historically ignored from GHG budgets estimates. This research also craves to expand the understanding of the CO2, CH4 and N2O emissions (diffusion and ebullition), evaluating the GHG production in sediments and the consume of methane by oxidation, and their relation to the oxygen profile in the water. In order to evaluade these factors, 9 artifical ponds were studied over eight months of measurements where 3 levels of eutrophication were created through P and N addition (high, intermediate and low nutrient loadings) and each pond was divided in quarters, which contained one of the different species in each one: floating plant (Azolla filiculoides) or three submerged plants (Ceratophyllum demersum, Elodea canadensis and Myriophyllum spicatum)...
Ação do ácido peracético e do hipoclorito de sódio no biofilme formado por Pseudomonas aeruginosa, isoladas da água de hemodiálise de um hospital público, na cidade de Botucatu
(Universidade Estadual Paulista (Unesp), 2016) Mozena, Lígia Berti [UNESP]; Rall, Vera Lúcia Mores [UNESP]; Universidade Estadual Paulista (Unesp)
Not available
Caracterização da avifauna presente em um Sistema Agroflorestal na Fazenda Experimental Lageado - Botucatu, SP
(Universidade Estadual Paulista (Unesp), 2016) Vieira, Jonas da Costa [UNESP]; Fonseca, Renata Cristina Batista [UNESP]; Universidade Estadual Paulista (Unesp)
Not available
Papel das metaloproteínases e das células satélites no músculo Sóleo durante o envelhecimento
(Universidade Estadual Paulista (Unesp), 2016) Valente, Jéssica Silvino [UNESP]; Pai, Maeli Dal [UNESP]; Universidade Estadual Paulista (Unesp)
Not available
Aspectos fisiológicos e morfoanatômicos de Tabebuia aurea submetida ao alagamento
(Universidade Estadual Paulista (Unesp), 2016) Coffacci, Lediane Roberta [UNESP]; Almeida, Luiz Fernando Rolim De [UNESP]; Universidade Estadual Paulista (Unesp)
Not available
Percepção química de patógenos na escolha de parceiros em tilápia-do-Nilo
(Universidade Estadual Paulista (Unesp), 2016) Mello, Isabela Maria de [UNESP]; Giaquinto, Percília Cardoso [UNESP]; Rossi, Vanessa Stramantinoli [UNESP]; Universidade Estadual Paulista (Unesp)
In sexual selection, males and females choose their partners based on characteristics associated with increased individual fitness and such sexual characteristics presented by males influence females' choice. The choice in fish can be performed by various sensory canals, including the chemical. Chemical signals emitted by males may contain indicators of health or genetic quality of potential partners. Thus, we tested if the female Nile tilapia realizes through chemical signals the presence of pathogens in males. To this end, females were individually tested in choice tests regarding two males, with one infected male and one healthy male. Most females (81.25%) visited faster and firstly (p=0.007) the compartment with chemical signs of healthy males. However, there was no difference regarding total time between the compartments (p=0.085). We conclude that there is evidence of females' perception of chemical signal of males, but it did not remain consistent over the choice
Distribuição e abundância dos ovos de resistência de cladocera em uma lagoa subtropical rasa marginal ao rio Paranapanema-SP
(Universidade Estadual Paulista (Unesp), 2016) Daré, Luana [UNESP]; Henry, Raoul [UNESP]; Castilho, Maria Carolina de Almeida [UNESP]; Portinho, Jorge Laço [UNESP]; Universidade Estadual Paulista (Unesp)
Not available
Dispersão de sementes pelo mico-leão-preto, Leontopithecus chrysopygus (Primates, Callitrichidae) em um fragmento de Mata Atlântica
(Universidade Estadual Paulista (Unesp), 2016-08) Alcolea, Mirela [UNESP]; Culot, Laurence M. V. [UNESP]; Rezende, Gabriela C. [UNESP]; Universidade Estadual Paulista (Unesp)
Primates can be efficient seed dispersers, having an important role on the regeneration of degraded areas and forest fragments. The aim of the study was to determine the role of the black lion tamarin (Leontopithecus chrysopygus) as a seed disperser through: 1) the characterization of the fruits consumed by the black-lion-tamarin and the identification of those that are dispersed; 2) the analysis of the spatial distribution of seeds dispersed and their dispersal distances; 3) the determination of the effect of gut passage on seed germination. We followed one group of black lion tamarins for 22 days in a fragment of Tropical Semideciduous Forest from March to September of 2015. We collected group behavior data, mapped the fruit trees they fed on and the locations of the feces, and collected the fruits consumed and the fecal samples. To determine the effect of gut passage on germination success, we compared the percentage of germination of defecated seeds, seeds with pulp, and without pulp. The black lion tamarins spent 25.6% of the time feeding, 85% of these feeding events being on fruits. They dispersed ten from the 11 species they consumed to a mean distance of 343.8 ± 225.8 m, within a home range of 82.9 ha. The spatial distribution of seeds dispersed was associated to the location of the feeding trees as well to the resting and sleeping trees. The passage of seeds through the black lion tamarins' gut significantly increased the germination success of 3 out of 9 tested species and had a neutral effect on the others. Although the study occurred during the dry season, a period of low fruit availability, fruits constituted a large part of the black lion tamarin's diet. In addition, the black lion tamarins dispersed the seeds of almost all of the species consumed within a large area and at long distances, which indicates that the species can be considered as an effective seed disperser...

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