Artigos - Engenharia Ambiental - São José dos Campos
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ItemArtigo Effect of cold atmospheric plasma jet associated to polyene antifungals on Candida albicans biofilms(MDPI, 2021-09-25) Koga-Ito, Cristiane Yumi [UNESP]; Leite, Lady Daiane Pereira; Oliveira, Maria Alcionéia Carvalho de; Vegian, Mariana Raquel da Cruz [UNESP]; Sampaio, Aline da Graça [UNESP]; Nishime, Thalita Mayumi Castaldelli [UNESP]; Kostov, Konstantin Georgiev; Universidade Estadual Paulista (Unesp): The increasing incidence of antifungal resistance represents a great challenge in the medical area and, for this reason, new therapeutic alternatives for the treatment of fungal infections are urgently required. Cold atmospheric plasma (CAP) has been proposed as a promising alternative technique for the treatment of superficial candidiasis, with inhibitory effect both in vitro and in vivo. However, little is known on the association of CAP with conventional antifungals. The aim of this study was to evaluate the effects of the association between CAP and conventional polyene antifungals on Candida albicans biofilms. C. albicans SC 5314 and a clinical isolate were used to grow 24 or 48 h biofilms, under standardized conditions. After that, the biofilms were exposed to nystatin, amphotericin B and CAP, separately or in combination. Different concentrations of the antifungals and sequences of treatment were evaluated to establish the most effective protocol. Biofilms viability after the treatments was compared to negative control. Data were compared by One-way ANOVA and post hoc Tukey (5%). The results demonstrate that 5 min exposure to CAP showed more effective antifungal effect on biofilms when compared to nystatin and amphotericin B. Additionally, it was detected that CAP showed similar (but smaller in magnitude) effects when applied in association with nystatin and amphotericin B at 40 µg/mL and 60 µg/mL. Therefore, it can be concluded that the application of CAP alone was more effective against C. albicans biofilms than in combination with conventional polyene antifungal agents.ItemArtigo Cold atmospheric plasma jet as a possible adjuvant therapy for periodontal disease(MDPI, 2021-09-15) Koga-ito, Cristiane Yumi [UNESP]; Lima, Gabriela de Morais Gouvêa [UNESP]; Borges, Aline Chiodi [UNESP]; Nishime, Thalita Mayumi Castaldelli; Santana-Melo, Gabriela de Fatima [UNESP]; Kostov, Konstantin Georgiev; Mayer, Marcia Pinto Alves; Universidade Estadual Paulista (Unesp): Due to the limitations of traditional periodontal therapies, and reported cold atmospheric plasma anti-inflammatory/antimicrobial activities, plasma could be an adjuvant therapy to periodontitis. Porphyromonas gingivalis was grown in blood agar. Standardized suspensions were plated on blood agar and plasma-treated for planktonic growth. For biofilm, dual-species Streptococcus gordonii + P. gingivalis biofilm grew for 48 h and then was plasma-treated. XTT assay and CFU counting were performed. Cytotoxicity was accessed immediately or after 24 h. Plasma was applied for 1, 3, 5 or 7 min. In vivo: Thirty C57BI/6 mice were subject to experimental periodontitis for 11 days. Immediately after ligature removal, animals were plasma-treated for 5 min once—Group P1 (n = 10); twice (Day 11 and 13)—Group P2 (n = 10); or not treated—Group S (n = 10). Mice were euthanized on day 15. Histological and microtomography analyses were performed. Significance level was 5%. Halo diameter increased proportionally to time of exposure contrary to CFU/mL counting. Mean/SD of fibroblasts viability did not vary among the groups. Plasma was able to inhibit P. gingivalis in planktonic culture and biofilm in a cell-safe manner. Moreover, plasma treatment in vivo, for 5 min, tends to improve periodontal tissue recovery, proportionally to the number of plasma applications.ItemArtigo Perspectives for an Elko phenomenology using monojets at the 14 TeV LHC(World Scientific Publ Co Pte Ltd, 2014-12-01) Alves, Alexandre; Dias, Marco André Ferreira Dias; Campos, Fernando Luiz de [UNESP]; Universidade Federal de São Paulo (UNIFESP); Universidade Estadual Paulista (Unesp)The aim of this work is to explore the possibility to discover a fermionic field with mass dimension one, the Elko field, in the 14 TeV Large Hadron Collider (LHC), in processes with missing energy and one jet. We explore the possibility of a triple coupling with the Higgs field, generating also a contribution to the Elko mass term, and suggest some possibilities for future studies in this field.