Estudo de populações de Rhodnius neglectus e R. prolixus (Hemiptera, Reduviidae, Triatominae) mantidas em laboratório por meio de marcadores mitocondriais, morfológicos e morfométricos
Carregando...
Data
2016-07-07
Autores
Falcone, Rossana [UNESP]
Título da Revista
ISSN da Revista
Título de Volume
Editor
Universidade Estadual Paulista (Unesp)
Resumo
Estima-se que 6 a 7 milhões de pessoas estejam infectadas com Trypanosoma cruzi no mundo, a maioria na América Latina, sendo a transmissão vetorial responsável ainda por, aproximadamente, 80% dos casos. Atualmente são descritas 151 espécies de triatomíneos agrupadas em cincos tribos formadas por 18 gêneros. O gênero Rhodnius conta com 19 espécies de potenciais vetores, podendo veicular T. cruzi e Trypanosoma rangeli. É considerado um dos gêneros taxonomicamente mais complexos da subfamília Triatominae, devido as semelhanças morfológicas e a distribuição geográfica que podem se sobrepor entre algumas de suas espécies dificultando seu estudo, apesar das diversas técnicas empregadas para sua distinção. Com o desenvolvimento atual das técnicas moleculares tornou-se possível a caracterização de espécies de triatomíneos por meio de marcadores mitocondriais e nucleares, tendo essa se tornado uma variável a mais para a taxonomia dos triatomíneos, assim como a já utilizada morfologia e a recentemente popularizada morfometria geométrica. A adição de novas variáveis possibilita que dados já existentes possam ser comprovados e/ou corrigidos. Neste trabalho foram obtidos subsídios que ampliam o conhecimento e que agregados aos já existentes podem proporcionar uma diferenciação mais minuciosa e precisa de espécies englobadas no gênero Rhodnius, de modo a contribuir para estabelecer uma classificação mais apropriada. A obtenção dos dados foi conduzida, por meio de estudo molecular, utilizando-se os marcadores mitocondriais – citocromo b e citocromo oxidase I, além de estudos morfológicos, utilizando-se como caracteres o processo mediano do pigóforo, o ângulo ânterolateral e a presença de colarinho nos ovos, a morfometria geométrica da cabeça também foi empregada, a fim de se distinguir populações de R. neglectus e R. prolixus. Os resultados obtidos a partir dessa análise multiparamétrica sugeriram que, as colônias identificadas como Rhodnius prolixus pertencem à espécie, que algumas colônias de R. neglectus analisadas apresentaram padrões condizentes com R. prolixus, já as demais apresentaram padrões condizentes com a espécie.
Worlwide, it is estimated that 6 to 7 million people are infected with Trypanosoma cruzi, mostly in Latin America and the vector transmission is still responsible for approximately 80% of the cases. There are currently 151 described species of triatomine, grouped into five tribes, formed by 18 genres. The Rhodnius genre includes 19 species, which can transmit T. cruzi and Trypanosoma rangeli. It is considered one of the most complex taxonomically genera of Triatominae, due to its morphological similarities and geographical distribution that may overlap among some species hindering their study, despite the various techniques used for their distinction. With the current development of molecular techniques it has become possible to characterize triatomine species through mitochondrial and nuclear markers, this became one more toll for the taxonomy of triatomines, just as the already used morphology and recently popularized geometric morphometry. The addition of new variables enables the verification and or correction of existing data. This work has generated valueable data that amplify the knowledge which in addition to the existing may provide a more accurate differentiation of species included in the genre Rhodnius, in order to help establish a more appropriate classification. Data collection was performed by DNA analysis, using mitochondrial markers - cytochrome b and cytochrome oxidase I, in addition to morphological parameters, as, median process of pygophore, the anterolateral angle and the presence of collar in eggs, geometric morphometry of the head was also used in order to help distinguish populations of R. neglectus and R. prolixus. The results obtained from this multiparameter analysis suggested that colonies identified as R. prolixus belong to the species, a few colonies of R. neglectus analyzed showed consistent patterns with R. prolixus, the others colonies of R. neglectus showed consistent patterns to the species originally identified.
Worlwide, it is estimated that 6 to 7 million people are infected with Trypanosoma cruzi, mostly in Latin America and the vector transmission is still responsible for approximately 80% of the cases. There are currently 151 described species of triatomine, grouped into five tribes, formed by 18 genres. The Rhodnius genre includes 19 species, which can transmit T. cruzi and Trypanosoma rangeli. It is considered one of the most complex taxonomically genera of Triatominae, due to its morphological similarities and geographical distribution that may overlap among some species hindering their study, despite the various techniques used for their distinction. With the current development of molecular techniques it has become possible to characterize triatomine species through mitochondrial and nuclear markers, this became one more toll for the taxonomy of triatomines, just as the already used morphology and recently popularized geometric morphometry. The addition of new variables enables the verification and or correction of existing data. This work has generated valueable data that amplify the knowledge which in addition to the existing may provide a more accurate differentiation of species included in the genre Rhodnius, in order to help establish a more appropriate classification. Data collection was performed by DNA analysis, using mitochondrial markers - cytochrome b and cytochrome oxidase I, in addition to morphological parameters, as, median process of pygophore, the anterolateral angle and the presence of collar in eggs, geometric morphometry of the head was also used in order to help distinguish populations of R. neglectus and R. prolixus. The results obtained from this multiparameter analysis suggested that colonies identified as R. prolixus belong to the species, a few colonies of R. neglectus analyzed showed consistent patterns with R. prolixus, the others colonies of R. neglectus showed consistent patterns to the species originally identified.
Descrição
Palavras-chave
Rhodnius, Taxonomia, Citocromo b, Morfologia, Morfometria geométrica, Cytochrome b, Morphology, Geometric morphometry